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Human fetal liver-derived CD7+CD2lowCD3-CD56- clones that express CD3
gamma, delta, and epsilon and proliferate in response to interleukin-2
(IL-2), IL-3, IL-4, or IL-7: implications for the relationship between T
and natural killer cells
T Hori, JH Phillips, B Duncan, LL Lanier and H Spits
DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA
94304-1104.
Cells from fetal liver or fetal and adult bone marrow that are membrane
(m)CD3 negative and have not rearranged TCR genes but express CD3 proteins
in their cytoplasm are considered to be committed T-cell progenitors.
Recent findings question whether CD3 is T-cell specific because fetal
natural killer (NK) cells have been shown to express cCD3 delta and epsilon
proteins. To further examine the relationship between T and NK cells, we
generated mCD3-cCD3+ clones from fetal liver. Two stable clones, FL412 and
FL508, isolated from different donors, were selected on the basis of
absence of the NK cell marker CD56. These clones shared a common phenotype
of CD7+CD2lowCD3-CD4-CD8-CD5-CD6- CD11b+CD16-CD56 -. Like fetal NK clones,
these clones expressed cytoplasmic CD3 delta and epsilon transcripts and
proteins. However, the clones exhibited no or very low levels of cytotoxic
activity against K562, JY, or Daudi, which were lysed efficiently by fetal
NK clones. TCR beta, gamma, and delta genes in these clones were in
germline configuration. Furthermore, both FL412 and FL508 responded not
only to interleukin-2 (IL-2), IL-4, or IL-7, but also to IL-3 with
proliferation. These results suggest that FL412 and FL508 retained some
characteristics of a putative T or NK precursor in the fetal liver and may
be useful for analyses of this poorly defined cell type.
Volume 80,
Issue 5,
pp. 1270-1278,
09/01/1992
Copyright © 1992 by The American Society of Hematology
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