|
|
 Previous Article | Table of Contents | Next Article 
The ABL-BCR fusion gene is expressed in chronic myeloid leukemia
JV Melo, DE Gordon, NC Cross and JM Goldman
Department of Haematology, Royal Postgraduate Medical School, London, UK.
Although the BCR-ABL hybrid gene on chromosome 22q-plays a pivotal role in
the pathogenesis of chronic myeloid leukemia (CML), little is known of the
reciprocal chimeric gene, ABL-BCR, formed on chromosome 9q+. By reverse
transcription/polymerase chain reaction amplification (RT/PCR) we have
detected ABL-BCR mRNA in cells from 31 of 44 BCR-ABL positive CML patients
and 3 of 5 CML cell lines. Of the 34 positive samples, 31 had classical
t(9;22) (q34;q11) translocations; in 3 samples there was no Philadelphia
(Ph) and/or 9q+ chromosomes. ABL-BCR expression consisted of ABL(Ib)-BCR
mRNA in 26 patients and of both ABL(Ib)-BCR and ABL(Ia)-BCR mRNA species in
6 patients. The ABL-BCR transcripts encoded one or, more rarely, both of
the two potential junctions, designated ABL-b3 and ABL-b4, which differed
in size by 75 bp. In 2 patients, the BCR exon b3 was not present in either
the BCR-ABL or the corresponding ABL-BCR transcript, whereas in 5 patients
exon b3 was present in both transcripts. Direct sequencing of PCR fragments
representing the full-length coding sequence of ABL-BCR cDNAs type Ib- b3,
Ia-b3, Ib-b4, and Ia-b4 showed an open reading frame predicted to encode
fusion proteins of 370 to 414 amino-acids. If an ABL-BCR gene product is
produced in CML cells, it may be relevant as a mechanism for deregulating
the GTPase activating protein (GAP) function of BCR.
Volume 81,
Issue 1,
pp. 158-165,
01/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Kreil, M. Pfirrmann, C. Haferlach, K. Waghorn, A. Chase, R. Hehlmann, A. Reiter, A. Hochhaus, N. C. P. Cross, and for the German Chronic Myelogenous Leukemia (CML)
Heterogeneous prognostic impact of derivative chromosome 9 deletions in chronic myelogenous leukemia
Blood,
August 15, 2007;
110(4):
1283 - 1290.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Chasseriau, J. Rivet, F. Bilan, J.-C. Chomel, F. Guilhot, N. Bourmeyster, and A. Kitzis
Characterization of the Different BCR-ABL Transcripts with a Single Multiplex RT-PCR
J. Mol. Diagn.,
November 1, 2004;
6(4):
343 - 347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Goldman and J. V. Melo
Chronic Myeloid Leukemia -- Advances in Biology and New Approaches to Treatment
N. Engl. J. Med.,
October 9, 2003;
349(15):
1451 - 1464.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. N. Deininger and B. J. Druker
Specific Targeted Therapy of Chronic Myelogenous Leukemia with Imatinib
Pharmacol. Rev.,
September 1, 2003;
55(3):
401 - 423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Saglio, C. T. Storlazzi, E. Giugliano, C. Surace, L. Anelli, G. Rege-Cambrin, A. Zagaria, A. J. Velasco, A. Heiniger, P. Scaravaglio, et al.
A 76-kb duplicon maps close to the BCR gene on chromosome 22 and the ABL gene on chromosome 9: Possible involvement in the genesis of the Philadelphia chromosome translocation
PNAS,
July 23, 2002;
99(15):
9882 - 9887.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. J. P. Huntly, A. J. Bench, E. Delabesse, A. G. Reid, J. Li, M. A. Scott, L. Campbell, J. Byrne, E. Pinto, A. Brizard, et al.
Derivative chromosome 9 deletions in chronic myeloid leukemia: poor prognosis is not associated with loss of ABL-BCR expression, elevated BCR-ABL levels, or karyotypic instability
Blood,
May 29, 2002;
99(12):
4547 - 4553.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Tipping, F. X. Mahon, V. Lagarde, J. M. Goldman, and J. V. Melo
Restoration of sensitivity to STI571 in STI571-resistant chronic myeloid leukemia cells
Blood,
December 15, 2001;
98(13):
3864 - 3867.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. de la Fuente, K. Merx, E. J. Steer, M. Muller, R. M. Szydlo, O. Maywald, U. Berger, R. Hehlmann, J. M. Goldman, N. C. P. Cross, et al.
ABL-BCR expression does not correlate with deletions on the derivative chromosome 9 or survival in chronic myeloid leukemia
Blood,
November 1, 2001;
98(9):
2879 - 2880.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Laurent, M. Talpaz, H. Kantarjian, and R. Kurzrock
The BCR Gene and Philadelphia Chromosome-positive Leukemogenesis
Cancer Res.,
March 1, 2001;
61(6):
2343 - 2355.
[Full Text]
|
|
|
|
|
|
|
B. J. Druker, C. L. Sawyers, R. Capdeville, J. M. Ford, M. Baccarani, and J. M. Goldman
Chronic Myelogenous Leukemia
Hematology,
January 1, 2001;
2001(1):
87 - 112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kantarjian, J. V. Melo, S. Tura, S. Giralt, and M. Talpaz
Chronic Myelogenous Leukemia: Disease Biology and Current and Future Therapeutic Strategies
Hematology,
January 1, 2000;
2000(1):
90 - 109.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Hussey, M. Nicola, S. Moore, G. B. Peters, and A. Dobrovic
The (4;11)(q21;p15) Translocation Fuses the NUP98 and RAP1GDS1 Genes and Is Recurrent in T-Cell Acute Lymphocytic Leukemia
Blood,
September 15, 1999;
94(6):
2072 - 2079.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J.A. ten Bosch, J. H. Kessler, A. M. Joosten, A. A. Bres-Vloemans, A. Geluk, B. C. Godthelp, J. van Bergen, C. J.M. Melief, and O. C. Leeksma
A BCR-ABL Oncoprotein p210b2a2 Fusion Region Sequence Is Recognized by HLA-DR2a Restricted Cytotoxic T Lymphocytes and Presented by HLA-DR Matched Cells Transfected With an Iib2a2 Construct
Blood,
August 1, 1999;
94(3):
1038 - 1045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Bose, M. Deininger, J. Gora-Tybor, J. M. Goldman, and J. V. Melo
The Presence of Typical and Atypical BCR-ABL Fusion Genes in Leukocytes of Normal Individuals: Biologic Significance and Implications for the Assessment of Minimal Residual Disease
Blood,
November 1, 1998;
92(9):
3362 - 3367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Beylot-Barry, A. Groppi, B. Vergier, K. Pulford, J.P. Merlio, and t. French Study Group of Cutaneous Lymphoma
Characterization of t(2;5) Reciprocal Transcripts and Genomic Breakpoints in CD30+ Cutaneous Lymphoproliferations
Blood,
June 15, 1998;
91(12):
4668 - 4676.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
