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Determination of adhesion force between single cell pairs generated by
activated GpIIb-IIIa receptors
KL Sung, MM Frojmovic, TE O'Toole, C Zhu, MH Ginsberg and S Chien
Department of AMES-Bioengineering, University of California, San Diego, La
Jolla 92093-0412.
A biophysical approach was used to directly determine the avidity of the
junction between two Chinese hamster ovary (CHO) cells bearing recombinant
GpIIb-IIIa in the presence and absence of fibrinogen. Micromanipulation was
used to induce conjugation of the cell pairs with or without activating the
GpIIb-IIIa molecules with monoclonal antibody (MoAb) 62. Activation of
GpIIb-IIIa caused an increase in the force required to separate the
conjugates. The molecular bonding force between cells bearing activated
GpIIb-IIIa and fibrinogen molecules was found to be 2.1 x 10(-7) dyne,
which is 3.7 times higher than that between nonactivated GpIIb-IIIa and
fibrinogen (5.7 x 10(-8) dyne). The results provide a quantitative
assessment of the molecular bonding force between fibrinogen and the
GpIIb-IIIa expressed on cell surface. The findings indicate that the
activation of GpIIb-IIIa leads to an increase in the adhesive force in CHO
cell aggregation by increasing the strength of the GpIIb-IIIa-fibrinogen
bonds rather than the number of these bonds.
Volume 81,
Issue 2,
pp. 419-423,
01/15/1993
Copyright © 1993 by The American Society of Hematology
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