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Type II interleukin-1 receptor is not expressed in cultured endothelial
cells and is not involved in endothelial cell activation
F Colotta, M Sironi, A Borre, T Pollicino, S Bernasconi, D Boraschi and A Mantovani
Centro Daniela e Catullo Borgomainerio, Istituto di Ricerche Farmacologiche
Mario Negri, Milano, Italy.
Interleukin-1 (IL-1) profoundly affects a number of functions of
endothelial cells (EC). It was previously shown that EC express the type I
80-Kd IL-1 receptor (IL-1 RI). In this study we define the expression and
functional significance of the type II IL-1R (IL-1 RII) in EC. Human
umbilical vein EC did not express appreciable levels of IL- 1 RII mRNA as
assessed by Northern analysis or reverse transcription and polymerase chain
reaction. Exposure to various cytokines (including IL-4, which augments
IL-1 RII in neutrophils) failed to induce IL-1 RII mRNA. The binding of
radiolabeled IL-1 beta to EC was blocked by antitype I (M4) but not by
antitype II (M22) monoclonal antibodies (MoAbs). MoAbs directed against the
IL-1 RI (M1 and M4) inhibited the induction of IL-6 and adhesion molecules
in EC by IL-1, whereas an anti- IL-1 RII (M22) was inactive. The human IL-1
receptor antagonist (IL- 1ra) preferentially interacts with IL-1 RI versus
IL-1 RII in the mouse. IL-1 ra inhibited the response of mouse endothelial
cells to IL- 1. We conclude that EC selectively express the IL-1 RI and
that this is involved in the response of this cell type to IL-1.
Volume 81,
Issue 5,
pp. 1347-1351,
03/01/1993
Copyright © 1993 by The American Society of Hematology
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