|
|
 Previous Article | Table of Contents | Next Article 
P-selectin mediates spontaneous leukocyte rolling in vivo
M Dore, RJ Korthuis, DN Granger, ML Entman and CW Smith
Speros P. Martel Laboratory of Leukocyte Biology, Department of Pediatrics,
Baylor College of Medicine, Houston, TX.
Rolling represents the initial step leading to leukocyte extravasation from
blood vessels during an inflammatory reaction. In vitro studies indicate
that P-selectin could be one of the ligands on endothelium involved in the
rolling phenomenon, although the molecular determinants responsible for
this transient attachment in vivo are still undefined. Our objectives were
to develop a blocking monoclonal antibody against canine P-selectin and to
use it to investigate the role of P-selectin in leukocyte rolling in vivo
using the technique of intravital microscopy. P-selectin was immunoaffinity
purified from canine platelets and used for the production of monoclonal
antibodies. One of the hybridomas generated, MD6, was shown by
enzyme-linked immunosorbent assay and by flow cytometry to bind
preferentially to stimulated platelets and to completely prevent binding of
stimulated platelets to neutrophils. Visualization of canine mesenteric
venules by intravital microscopy showed that administration of MD6 resulted
in a marked inhibition in the number of rolling leukocytes (18.96 +/- 9.92
v 156.40 +/- 19.50 leukocytes/min, P < .05; 88.3% +/- 6.0% inhibition).
Control antibody MD3 (which recognizes a nonfunctional epitope of canine P-
selectin) had no effect on the number of rolling leukocytes or on their
rolling velocity. These results show for the first time that P-selectin
plays an essential role in leukocyte rolling in vivo, and therefore may be
a key participant of the inflammatory response.
Volume 82,
Issue 4,
pp. 1308-1316,
08/15/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Zarbock, H. Muller, Y. Kuwano, and K. Ley
PSGL-1-dependent myeloid leukocyte activation
J. Leukoc. Biol.,
November 1, 2009;
86(5):
1119 - 1124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Miyahara, L. Almulki, K. Noda, T. Nakazawa, T. Hisatomi, S. Nakao, K. L. Thomas, A. Schering, S. Zandi, S. Frimmel, et al.
In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis
FASEB J,
June 1, 2008;
22(6):
1973 - 1980.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Patrick, J. Rullo, S. Beaudin, P. Liaw, and A. E. Fox-Robichaud
Hepatic leukocyte recruitment in response to time-limited expression of TNF-{alpha} and IL-1beta
Am J Physiol Gastrointest Liver Physiol,
October 1, 2007;
293(4):
G663 - G672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Li, R. E. Rumbaut, A. R. Burns, and C. W. Smith
Platelet Response to Corneal Abrasion Is Necessary for Acute Inflammation and Efficient Re-epithelialization
Invest. Ophthalmol. Vis. Sci.,
November 1, 2006;
47(11):
4794 - 4802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Egami, T. Murohara, M. Aoki, and T. Matsuishi
Ischemia-induced angiogenesis: role of inflammatory response mediated by P-selectin
J. Leukoc. Biol.,
May 1, 2006;
79(5):
971 - 976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. R. Hicks, K. B. Abbitt, P. Dodd, V. C. Ridger, P. G. Hellewell, and K. E. Norman
The anti-inflammatory effects of a selectin ligand mimetic, TBC-1269, are not a result of competitive inhibition of leukocyte rolling in vivo
J. Leukoc. Biol.,
January 1, 2005;
77(1):
59 - 66.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nishijima, J. Kiryu, A. Tsujikawa, K. Miyamoto, M. Honjo, H. Tanihara, A. Nonaka, K. Yamashiro, H. Katsuta, S. Miyahara, et al.
Platelets Adhering to the Vascular Wall Mediate Postischemic Leukocyte-Endothelial Cell Interactions in Retinal Microcirculation
Invest. Ophthalmol. Vis. Sci.,
March 1, 2004;
45(3):
977 - 984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Blann, S. K. Nadar, and G. Y.H. Lip
The adhesion molecule P-selectin and cardiovascular disease
Eur. Heart J.,
December 2, 2003;
24(24):
2166 - 2179.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nishiwaki, T. Ueda, S. Ugawa, S. Shimada, and Y. Ogura
Upregulation of P-selectin and Intercellular Adhesion Molecule-1 after Retinal Ischemia-Reperfusion Injury
Invest. Ophthalmol. Vis. Sci.,
November 1, 2003;
44(11):
4931 - 4935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K Yamashiro, J Kiryu, A Tsujikawa, A Nonaka, K Nishijima, H Kamizuru, K Miyamoto, Y Honda, T Jomori, and Y Ogura
Suppressive effects of selectin inhibitor SKK-60060 on the leucocyte infiltration during endotoxin induced uveitis
Br J Ophthalmol,
April 1, 2003;
87(4):
476 - 480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nishijima, J. Kiryu, A. Tsujikawa, M. Honjo, A. Nonaka, K. Yamashiro, H. Kamizuru, Y. Ieki, H. Tanihara, Y. Honda, et al.
Inhibitory Effects of Antithrombin III on Interactions between Blood Cells and Endothelial Cells during Retinal Ischemia-Reperfusion Injury
Invest. Ophthalmol. Vis. Sci.,
January 1, 2003;
44(1):
332 - 341.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. E. Burch, V. R. S. Patil, R. T. Camphausen, M. F. Kiani, and D. J. Goetz
The N-terminal peptide of PSGL-1 can mediate adhesion to trauma-activated endothelium via P-selectin in vivo
Blood,
June 28, 2002;
100(2):
531 - 538.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamashiro, J. Kiryu, A. Tsujikawa, M. Honjo, A. Nonaka, K. Miyamoto, Y. Honda, H. Tanihara, and Y. Ogura
Inhibitory Effects of Antithrombin III against Leukocyte Rolling and Infiltration during Endotoxin-Induced Uveitis in Rats
Invest. Ophthalmol. Vis. Sci.,
June 1, 2001;
42(7):
1553 - 1560.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Tremblay, M. Paradis, and M. Dore
Expression of E- and P-selectin in Tumor Necrosis Factor-induced Dermatitis in Dogs
Vet. Pathol.,
May 1, 2001;
38(3):
261 - 268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. M. Amin, S. Ahmad, J. M. Walenga, D. A. Hoppensteadt, H. Leitz, and J. Fareed
Soluble P-Selectin in Human Plasma: Effect of Anticoagulant Matrix and its Levels in Patients With Cardiovascular Disorders
Clinical and Applied Thrombosis/Hemostasis,
April 1, 2000;
6(2):
71 - 76.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Arai, Y. Masui, P. Goldschmidt-Clermont, A. DiPaula, C. Siu, T. Kondo, and L. C. Becker
P-selectin inhibition prevents early neutrophil activation but provides only modest protection against myocardial injury in dogs with ischemia and forty-eight hours reperfusion
J. Am. Coll. Cardiol.,
July 1, 1999;
34(1):
280 - 288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Jung and K. Ley
Mice Lacking Two or All Three Selectins Demonstrate Overlapping and Distinct Functions for Each Selectin
J. Immunol.,
June 1, 1999;
162(11):
6755 - 6762.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Sakurai, F. C. Schmalstieg, L. D. Traber, H. K. Hawkins, and D. L. Traber
Role of L-selectin in physiological manifestations after burn and smoke inhalation injury in sheep
J Appl Physiol,
April 1, 1999;
86(4):
1151 - 1159.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Eguchi, H. Ikeda, T. Murohara, H. Yasukawa, N. Haramaki, S. Sakisaka, and T. Imaizumi
Endothelial Injuries of Coronary Arteries Distal to Thrombotic Sites : Role of Adhesive Interaction Between Endothelial P-Selectin and Leukocyte Sialyl LewisX
Circ. Res.,
March 19, 1999;
84(5):
525 - 535.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hafezi-Moghadam and K. Ley
Relevance of L-selectin Shedding for Leukocyte Rolling In Vivo
J. Exp. Med.,
March 15, 1999;
189(6):
939 - 948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.I. Rojas and A.R. Ahmed
Adhesion Receptors in Health and Disease
Critical Reviews in Oral Biology & Medicine,
January 1, 1999;
10(3):
337 - 358.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Palazzo, S. P. Jones, D. C. Anderson, D. N. Granger, and D. J. Lefer
Coronary endothelial P-selectin in pathogenesis of myocardial ischemia-reperfusion injury
Am J Physiol Heart Circ Physiol,
November 1, 1998;
275(5):
H1865 - H1872.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Papi, B. Didona, O. De Pita, A. Frezzolini, S. Di Giulio, W. De Matteis, D. Del Principe, and R. Cavalieri
Livedo Vasculopathy vs Small Vessel Cutaneous Vasculitis: Cytokine and Platelet P-Selectin Studies
Arch Dermatol,
April 1, 1998;
134(4):
447 - 452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Martinsson, T. Oda, E. Fernvik, K. Roempke, C.-J. Dalsgaard, and E. Svensjö
Ropivacaine Inhibits Leukocyte Rolling, Adhesion and CD11b/CD18 Expression
J. Pharmacol. Exp. Ther.,
October 1, 1997;
283(1):
59 - 65.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. Provost, J. Tremblay, and Y. Merhi
The Antiadhesive and Antithrombotic Effects of the Nitric Oxide Donor SIN-1 Are Combined With a Decreased Vasoconstriction in a Porcine Model of Balloon Angioplasty
Arterioscler Thromb Vasc Biol,
September 1, 1997;
17(9):
1806 - 1812.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. Borges, R. Eytner, T. Moll, M. Steegmaier, M. A. Campbell, K. Ley, H. Mossmann, and D. Vestweber
The P-Selectin Glycoprotein Ligand-1 Is Important for Recruitment of Neutrophils Into Inflamed Mouse Peritoneum
Blood,
September 1, 1997;
90(5):
1934 - 1942.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. H. Reinhardt, J. F. Elliott, and P. Kubes
Neutrophils Can Adhere Via alpha 4beta 1 -Integrin Under Flow Conditions
Blood,
May 15, 1997;
89(10):
3837 - 3846.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Ramos, E. J. Kunkel, M. B. Lawrence, U. Jung, D. Vestweber, R. Bosse, K. W. McIntyre, K. M. Gillooly, C. R. Norton, B. A. Wolitzky, et al.
Differential Effect of E-Selectin Antibodies on Neutrophil Rolling and Recruitment to Inflammatory Sites
Blood,
April 15, 1997;
89(8):
3009 - 3018.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kanwar, D. C. Bullard, M. J. Hickey, C. W. Smith, A. L. Beaudet, B. A. Wolitzky, and P. Kubes
The Association between {alpha}4-Integrin, P-Selectin, and E-Selectin in an Allergic Model of Inflammation
J. Exp. Med.,
March 17, 1997;
185(6):
1077 - 1088.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Liao, R. M. Starzyk, and D. N. Granger
Molecular Determinants of Oxidized Low-Density Lipoprotein–Induced Leukocyte Adhesion and Microvascular Dysfunction
Arterioscler Thromb Vasc Biol,
March 1, 1997;
17(3):
437 - 444.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E.R. Damiano, J. Westheider, A. Tozeren, and K. Ley
Variation in the Velocity, Deformation, and Adhesion Energy Density of Leukocytes Rolling Within Venules
Circ. Res.,
December 1, 1996;
79(6):
1122 - 1130.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. J. Kunkel and K. Ley
Distinct Phenotype of E-Selectin–Deficient Mice: E-Selectin Is Required for Slow Leukocyte Rolling In Vivo
Circ. Res.,
December 1, 1996;
79(6):
1196 - 1204.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. L. Entman and C. M. Ballantyne
Association of Neutrophils With Platelet Aggregates in Unstable Angina: Should We Alter Therapy?
Circulation,
September 15, 1996;
94(6):
1206 - 1208.
[Full Text]
|
|
|
|
|
|
|
J. Panes, I. Kurose, M. D. Rodriguez-Vaca, D. C. Anderson, M. Miyasaka, P. Tso, and D. N. Granger
Diabetes Exacerbates Inflammatory Responses to Ischemia-Reperfusion
Circulation,
January 1, 1996;
93(1):
161 - 167.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. C. Ridings, G. L. Bloomfield, S. Holloway, A. C. J. Windsor, M. A. Jutila, A. A. Fowler III, and H. J. Sugerman
Sepsis-Induced Acute Lung Injury Is Attenuated by Selectin Blockade Following the Onset of Sepsis
Arch Surg,
November 1, 1995;
130(11):
1199 - 1208.
[Abstract]
[PDF]
|
|
|
|
|
