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In vitro autoradiographic and in vivo scintigraphic localization of
somatostatin receptors in human lymphatic tissue
JC Reubi, B Waser, U Horisberger, E Krenning, SW Lamberts, JO Gebbers, P Gersbach and JA Laissue
Division of Cell Biology and Experimental Cancer Research, University of
Berne, Switzerland.
Receptors for the neuropeptide somatostatin (SS) were evaluated in vitro
and in vivo in various human lymphatic tissues, ie, thymus, spleen, and
lymph nodes; thymic carcinoids and thymomas were also tested. The receptors
were measured in vitro using receptor autoradiography on tissue sections
incubated with the SS analog 125I- [Tyr3]-octreotide or
125I-[Leu8,D-Trp22,Tyr25]-SS-28. All tissues were SS-receptor positive for
either radioligand, except the thymomas. In thymic tissue, the receptors
were diffusely located in the medulla, presumably on epithelial cells. In
the spleen, the red pulp was strongly labeled. In the lymph nodes, the
germinal centers were preferentially labeled. In all tissues, the receptors
were of high affinity (kd thymus, 0.84 nmol/L; kd spleen, 1.6 nmol/L; kd
lymph node, 0.62 nmol/L) and specific for SS. Displacement by nanomolar
concentrations of SS-14, SS-28, and octreotide was observed, as was
guanosine triphosphate dependency. The in vivo visualization of
somatostatin receptors was performed after injection of 111In-DTPA-
octreotide and gamma-camera scintigraphy. The spleen, but not thymus or
lymph nodes, were visualized. These data suggest an important role for SS
in regulating immune functions through SS receptors in thymus, spleen, and
lymph nodes. Furthermore, SS may regulate neuroendocrine functions in the
thymus.
Volume 82,
Issue 7,
pp. 2143-2151,
10/01/1993
Copyright © 1993 by The American Society of Hematology
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