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Both megakaryocytopoiesis and erythropoiesis are induced in mice infected
with a retrovirus expressing an oncogenic erythropoietin receptor [see
comments]
GD Longmore, P Pharr, D Neumann and HF Lodish
Department of Medicine, Washington University School of Medicine, St Louis,
MO 63110.
Increasing direct and indirect evidence suggests that erythropoietin (Epo)
promotes both erythropoiesis and megakaryocytopoiesis. Here we report that,
in mice infected with a recombinant spleen focus-forming retrovirus (SFFV)
expressing an oncogenic erythropoietin receptor (EpoR), there was an
increase in platelet count preceding the ensuing erythrocytosis.
Concurrently, there was a substantial increase in splenic megakaryocytes.
Culture of the bone marrow and spleen cells from infected mice showed
enhanced numbers of multipotent megakaryocytic progenitors. DNA polymerase
chain reaction analysis of individual megakaryocyte-containing colonies
showed recombinant SFFV (SFFVcEpoR) proviral integration.
Immunofluorescence of spleen sections showed overexpression of EpoR protein
in the megakaryocytes. Mice infected with a strain of SFFV also developed
splenic megakaryocytosis without activating overexpression of the EpoR in
megakaryocytes. This in vivo system shows that a relationship between
erythropoiesis and thrombopoiesis can exist at the level of the Epo-EpoR
signaling pathway. Also, SFFV-based vectors may be excellent vehicles for
the introduction of genes into multipotent, hematopoietic progenitors, in
vitro.
Volume 82,
Issue 8,
pp. 2386-2395,
10/15/1993
Copyright © 1993 by The American Society of Hematology
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