|
|
 Previous Article | Table of Contents | Next Article 
Binding of membrane-anchored macrophage colony-stimulating factor (M- CSF)
to its receptor mediates specific adhesion between stromal cells and M-CSF
receptor-bearing hematopoietic cells
N Uemura, K Ozawa, K Takahashi, A Tojo, K Tani, K Harigaya, S Suzu, K Motoyoshi, H Matsuda and H Yagita
Department of Hematology-Oncology, University of Tokyo, Japan.
To explore the biologic significance of the membrane-anchored form of
macrophage colony-stimulating factor (M-CSF), we examined whether
interaction between membrane-bound M-CSF and its receptor mediates
intercellular adhesion as well as cell proliferation and differentiation.
Human M-CSF receptors were expressed on a murine interleukin-2
(IL-3)-dependent cell line, FDC-P2, by DNA transfection with the c-fms gene
(FDC-P2-MCSFR). A human bone marrow-derived stromal cell line, KM102, was
used in the cell adhesion assay. The expression of membrane-bound M-CSF on
KM102 cells was demonstrated by flow cytometry and immunoblot analysis.
After the incubation of parent and transformed FDC-P2 cells on confluent
KM102 cells, nonadherent cells were removed and the cells attached to KM102
cells were examined microscopically. Almost all parent FDC-P2 cells were
nonadherent, whereas a significant number of FDC-P2-MCSFR cells bound to
KM102 cells. The addition of anti-M-CSF or anti-M-CSF receptor neutralizing
antibodies dose-dependently inhibited the binding of [3H]-thymidine-
labeled FDC-P2-MCSFR cells to KM102 cells. An excess amount of M-CSF also
inhibited the binding. On the other hand, the addition of antibodies
against some representative adhesion molecules (vitronectin receptor,
Pgp-1/CD44, and VLA-4) did not inhibit the adhesion between FDC-P2-MCSFR
cells and KM102 cells. These results support the idea that
membrane-anchored M-CSF and its receptor may function as mediators of
cell-cell adhesion.
Volume 82,
Issue 9,
pp. 2634-2640,
11/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Wang, G.-G. Zheng, C.-H. Ma, Y.-M. Lin, H.-Y. Zhang, Y.-Y. Ma, J.-H. Chong, and K.-F. Wu
A Special Linker between Macrophage and Hematopoietic Malignant Cells: Membrane Form of Macrophage Colony-Stimulating Factor
Cancer Res.,
July 15, 2008;
68(14):
5639 - 5647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. M. Mitrasinovic, A. Grattan, C. C. Robinson, N. B. Lapustea, C. Poon, H. Ryan, C. Phong, and G. M. Murphy Jr
Microglia Overexpressing the Macrophage Colony-Stimulating Factor Receptor Are Neuroprotective in a Microglial-Hippocampal Organotypic Coculture System
J. Neurosci.,
April 27, 2005;
25(17):
4442 - 4451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-M. Dai, X.-H. Zong, V. Sylvestre, and E. R. Stanley
Incomplete restoration of colony-stimulating factor 1 (CSF-1) function in CSF-1-deficient Csf1op/Csf1op mice by transgenic expression of cell surface CSF-1
Blood,
February 1, 2004;
103(3):
1114 - 1123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ide, D. B. Seligson, S. Memarzadeh, L. Xin, S. Horvath, P. Dubey, M. B. Flick, B. M. Kacinski, A. Palotie, and O. N. Witte
Expression of colony-stimulating factor 1 receptor during prostate development and prostate cancer progression
PNAS,
October 29, 2002;
99(22):
14404 - 14409.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Fan, D. Fan, H. Gewant, C. L. Royce, M. S. Nanes, and J. Rubin
Increasing membrane-bound MCSF does not enhance OPGL-driven osteoclastogenesis from marrow cells
Am J Physiol Endocrinol Metab,
January 1, 2001;
280(1):
E103 - E111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Rubin, D. M. Biskobing, L. Jadhav, D. Fan, M. S. Nanes, S. Perkins, and X. Fan
Dexamethasone Promotes Expression of Membrane-Bound Macrophage Colony-Stimulating Factor in Murine Osteoblast-Like Cells
Endocrinology,
March 1, 1998;
139(3):
1006 - 1012.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Richard, J. P. Liuzzo, and D. Moscatelli
Fibroblast Growth Factor-2 Can Mediate Cell Attachment by Linking Receptors and Heparan Sulfate Proteoglycans on Neighboring Cells
J. Biol. Chem.,
October 13, 1995;
270(41):
24188 - 24196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
