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A reverse transcriptase-polymerase chain reaction detects heterogeneous
chimeric mRNAs in leukemias with 11q23 abnormalities
K Yamamoto, M Seto, S Iida, H Komatsu, N Kamada, S Kojima, Y Kodera, S Nakazawa, H Saito and T Takahashi
Laboratories of Chemotherapy and Immunology, Aichi Cancer Center Research
Institute, Nagoya, Japan.
The MLL gene involved in 11q23 translocations found in the majority of
infantile leukemias and some secondary leukemias makes fusion transcripts
with genes such as LTG4 (chromosome 4), LTG9 (chromosome 9), and LTG19
(chromosome 19) as a result of reciprocal translocation. We have examined
25 cases of leukemias with 11q23 abnormalities by Southern blot analysis
and the reverse transcriptase-polymerase chain reaction (RT-PCR). Using
various primer pairs, chimeric mRNAs could be amplified in 6 of 7 leukemias
with t(4;11), 6 of 8 leukemias with t(9;11) including secondary leukemia, 8
of 9 leukemias with t(11;19), and 1 with a deletion at 11q23. The chimeric
mRNAs were heterogeneous and differential usage of the MLL exons was found,
irrespective of the partner chromosomes. Sensitivity studies showed that a
single clone with chimeric mRNA in 10(4) to 10(5) cells could be detected.
These findings show that the present RT-PCR settings provide a rapid,
accurate, and sensitive tool for diagnosing leukemias with 11q23
translocations and for monitoring response to therapy in these patients.
Volume 83,
Issue 10,
pp. 2912-2921,
05/15/1994
Copyright © 1994 by The American Society of Hematology
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