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The immunophenotype of splenic lymphoma with villous lymphocytes and its
relevance to the differential diagnosis with other B-cell disorders
E Matutes, R Morilla, K Owusu-Ankomah, A Houlihan and D Catovsky
Academic Department of Haematology and Cytogenetics, Royal Marsden
Hospital, London, UK.
Splenic lymphoma with villous lymphocytes (SLVL) is a low-grade disorder
that regularly presents with peripheral blood involvement. We describe the
immunophenotype of the circulating cells from 100 SLVL patients whose
disease has been characterized on clinical, morphologic, and histologic
grounds. Cells from all cases expressed B-cell antigens (CD19 and CD37)
and/or HLA-Dr and showed light chain restriction (kappa/lambda: 1.5/1) with
moderate to strong intensity of membrane Ig staining. Cells from most cases
(> 80%) were CD24+, FMC7+, and expressed strongly membrane CD22. The
monoclonal antibodies CD10, CD23, and CD38 were positive in one-third of
the cases; CD11c in 47%; and CD25 in 25% of cases. A minority of cases
(< 20%) were positive with HC2, B-ly-7, and CD5. However, none of the 19
CD5+ cases had the phenotype characteristic of chronic lymphocytic leukemia
(CD5+, CD23+, FMC7-, weak surface Ig and membrane CD22). None of the 17
CD25+ cases had the immunophenotype typical of hairy cell leukemia (CD25+,
CD11c+, HC2+, B-ly-7+). HC2 and B-ly-7 were the most useful reagents to
distinguish SLVL from hairy cell leukemia. Our findings demonstrate that
SLVL has a distinct immunologic profile and that monoclonal antibodies are
important for the differential diagnosis between this disease and other
B-lymphoproliferative disorders with which SLVL can be confused.
Volume 83,
Issue 6,
pp. 1558-1562,
03/15/1994
Copyright © 1994 by The American Society of Hematology
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