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Differential attachment of human neoplastic B cells to purified
extracellular matrix molecules
D Segat, C Pucillo, G Marotta, R Perris and A Colombatti
Divisione di Oncologia Sperimentale 2, CRO, Aviano, Italy.
Recirculation of normal and neoplastic lymphocytes occurs via binding to
the endothelial luminar surface, followed by extravasation and the
subsequent interaction of the cells with components of the underlying
basement membrane and stromal extracellular matrix (ECM). To identify
matrix constituents that could be involved in the tissue dissemination of
neoplastic B cells, we have examined the ability of three lymphoma B- cell
lines and one Philadelphia chromosome (Ph1)-positive cell line established
from the lymphoid transformation of a chronic myeloid leukemia (CML) to
adhere to a range of purified ECM molecules. Immunophenotyping with a panel
of markers suggested that the lines derived from cells that had undergone
transformation at distinct stages of B-cell maturation. The four cell lines
displayed a differential ability to adhere to the ECM molecules tested.
BV-173, Ci-1, and Sc-1 cells attached to various degrees to fibronectin
(FN). Ri-1, Ci-1, and Sc-1 cells attached to human laminin (LN) variants,
whereas only Ci-1 and Sc-1 cells showed some affinity for collagen (Col)
type VI. All four cell lines interacted with fibrillar Col I, but only
BV-173 and Ri- 1 cells attached to fibrillar Col III. The subendothelial
Col VIII only was active as a substratum for BV-173 cells. In all cases,
cells bound to fibrillar collagens when they were assembled into polymeric
fibrils, and were incapable of adhering to monomeric and denatured
collagen. In contrast to cell adhesion to FN and LN, which showed a plateau
at high substrate concentrations, adhesion to fibrillar Col I reached a
peak at intermediary concentrations and decreased thereafter, suggesting
that cells respond to a definite macromolecular arrangement of collagenous
fibrils. Adhesion to individual ECM molecules was not directly correlated
with the apparent maturation state of the cells, nor with the relative
density of known ECM receptors. Taken together, these results suggest that
interaction of neoplastic B cells with selected matrix components may
influence their dispersion throughout tissues. We further suggest that the
use of quantitative cell adhesion assays in vitro may provide means of
defining the behavioral traits of neoplastic B cells in vivo.
Volume 83,
Issue 6,
pp. 1586-1594,
03/15/1994
Copyright © 1994 by The American Society of Hematology
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