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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chelstrom, L. Articles by Uckun, F. Search for Related Content PubMed PubMed Citation Articles by Chelstrom, L. Articles by Uckun, F. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Childhood acute myeloid leukemia in mice with severe combined immunodeficiency LM Chelstrom, R Gunther, J Simon, SC Raimondi, R Krance, WM Crist and FM Uckun Department of Therapeutic Radiology-Radiation Oncology, University of Minnesota, Minneapolis. Primary bone marrow blasts from 4 children with t(8;21) acute myeloid leukemia (AML), 6 children with inv(16) AML, and 2 children with t(9;11) AML were injected intravenously or transplanted under the kidney capsule of sublethally irradiated mice with severe combined immunodeficiency (SCID). Leukemic cells from all AML patients infiltrated the SCID mouse thymus, suggesting that the thymic microenvironment supports the survival and growth of human AML blasts. Blasts from 1 of 4 t(8;21) AML patients and 4 of 6 inv(16) AML patients caused histopathologically detectable disseminated leukemia. Blasts from the remaining patients produced disseminated occult leukemia that was only detected by polymerase chain reaction. Occurrence of histopathologically detectable disseminated leukemia was dependent on intravenous injection of leukemic cells; none of the mice challenged with an inoculum transplanted under the kidney capsule developed overt leukemia. No obvious association was noted between occurrence of leukemia in SCID mice and clinical or laboratory features presented by patients, including age, sex, or leukocyte count at diagnosis. To our knowledge, this study is the first to show that leukemic blasts from children with newly diagnosed AML, especially inv(16) AML, can cause disseminated human leukemia in SCID mice without exogenous cytokine support. The SCID mouse model system may prove particularly useful for designing more effective treatment strategies against childhood AML. Volume 84, Issue 1, pp. 20-26, 07/01/1994 Copyright © 1994 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. P.C. Steele, R. D. Clutterbuck, R. L. Powles, P. L.R. Mitchell, C. Horton, R. Morilla, D. Catovsky, and J. L. Millar Growth of Human T-Cell Lineage Acute Leukemia in Severe Combined Immunodeficiency (SCID) Mice and Non-obese Diabetic SCID Mice Blood, September 1, 1997; 90(5): 2015 - 2019. [Abstract] [Full Text] [PDF]

	Copyright © 1994 by American Society of Hematology         Online ISSN: 1528-0020