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Human fetal bone marrow early progenitors for T, B, and myeloid cells are
found exclusively in the population expressing high levels of CD34
D DiGiusto, S Chen, J Combs, S Webb, R Namikawa, A Tsukamoto, BP Chen and AH Galy
Cell Biology, Division Group, Systemix Inc, Palo Alto, CA 94304.
Experimentation on human stem cells is hampered by the relative paucity of
this population and by the lack of assays identifying multilineage
differentiation, particularly along the lymphoid lineages. In our current
study, phenotypic analysis of low-density fetal bone marrow cells showed
two distinct populations of CD34+ cells: those expressing a high density of
CD34 antigen on their surface (CD34hi) and those expressing an intermediate
level of CD34 antigen (CD34lo). Multiple tissues were used to characterize
the in vitro and in vivo potential of these subsets and showed that only
CD34hi cells support long-term B lymphopoiesis and myelopoiesis in vitro
and mediate T, B, and myeloid repopulation of human tissues implanted into
SCID mice. CD34lo cells repeatedly failed to provide long-term
hematopoietic activity in vivo or in vitro. These results indicate that a
simple fractionation based on well-defined CD34 antigen levels can be used
to reproducibly isolate cells highly enriched for in vivo long-term
repopulating activity and for multipotent progenitors, including T- and
B-cell precursors. Additionally, given the limited variability in the
results and the high correlation between in vitro and in vivo hematopoietic
potential, we propose that the CD34hi population contains virtually all of
the stem cell activity in fetal bone marrow and therefore is the population
of choice for future studies in hematopoietic stem cell development and
gene therapy.
Volume 84,
Issue 2,
pp. 421-432,
07/15/1994
Copyright © 1994 by The American Society of Hematology
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