|
|
 Previous Article | Table of Contents | Next Article 
A subset of human CD34+ hematopoietic progenitors express low levels of
CD4, the high-affinity receptor for human immunodeficiency virus-type 1
G Zauli, G Furlini, M Vitale, MC Re, D Gibellini, L Zamai, G Visani, P Borgatti, S Capitani and M La Placa
Institute of Human Anatomy, University of Ferrara, Italy.
We investigated the expression of CD4 antigen in normal bone marrow (BM)
samples, enriched in CD34+ hematopoietic progenitor cells. At flow
cytometry, a significant fraction (ranging from 25% to 65%) of CD34+ cells
also showed low levels of CD4 antigen on their surface. The CD4 receptor
densities on the surface of hematopoietic progenitors was approximately 50%
that of peripheral blood monocytes and 5% of peripheral blood T
lymphocytes. In immunoprecipitation experiments, the CD4 antigen expressed
by BM hematopoietic progenitors appeared to be the same form expressed by
mature peripheral blood CD4+ cells and appeared to be a potentially
functional receptor for human immunodeficiency virus-type 1, because it
specifically bound recombinant envelope gp120. Moreover, BM samples, highly
enriched in CD34+ cells, showed the presence of CD4 mRNA at reverse
transcription- polymerase chain reaction examination. In experiments of
complement- mediated cytotoxicity with Leu3-a+Leu3b anti-CD4 monoclonal
antibody, a significant reduction in the number of both classes of
megakaryocyte (burst-forming unit-meg [BFU-meg] and colony-forming unit-meg
[CFU- meg]) and granulocyte/macrophage (CFU-GM) progenitors was observed,
whereas erythroid (BFU-E) progenitors were only slightly affected.
Moreover, purified CD4+ BM cells obtained by immunomagnetic selection,
using high concentrations of Leu3a+Leu3b, showed a colony-forming ability
of megakaryocyte and granulocyte/macrophage progenitors comparable with
that of CD4- BM cells. In conclusion, the present data show that immature
hematopoietic progenitor cells express low levels of CD4, the high-affinity
receptor of human immunodeficiency virus-type 1.
Volume 84,
Issue 6,
pp. 1896-1905,
09/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. Biswas, B. Mantelli, A. Sica, M. Malnati, C. Panzeri, A. Saccani, H. Hasson, A. Vecchi, A. Saniabadi, P. Lusso, et al.
Expression of CD4 on human peripheral blood neutrophils
Blood,
June 1, 2003;
101(11):
4452 - 4456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Marodon, E. Mouly, E. J. Blair, C. Frisen, F. M. Lemoine, and D. Klatzmann
Specific transgene expression in human and mouse CD4+ cells using lentiviral vectors with regulatory sequences from the CD4 gene
Blood,
May 1, 2003;
101(9):
3416 - 3423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Bertagnolo, M. Marchisio, S. Pierpaoli, M. L. Colamussi, F. Brugnoli, G. Visani, G. Zauli, and S. Capitani
Selective up-regulation of phospholipase C-{beta}2 during granulocytic differentiation of normal and leukemic hematopoietic progenitors
J. Leukoc. Biol.,
June 1, 2002;
71(6):
957 - 965.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, L. Li, R. Wadley, S. W. Reddel, J. C. Qi, C. Archis, A. Collins, E. Clark, M. Cooley, S. Kouts, et al.
Mast cells/basophils in the peripheral blood of allergic individuals who are HIV-1 susceptible due to their surface expression of CD4 and the chemokine receptors CCR3, CCR5, and CXCR4
Blood,
June 1, 2001;
97(11):
3484 - 3490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. T. Scadden, H. Shen, and T. Cheng
Hematopoietic Stem Cells in HIV Disease
J Natl Cancer Inst Monographs,
December 1, 2000;
2000(28):
24 - 29.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Mirandola, P. Secchiero, S. Pierpaoli, G. Visani, L. Zamai, M. Vitale, S. Capitani, and G. Zauli
Infection of CD34+ hematopoietic progenitor cells by human herpesvirus 7 (HHV-7)
Blood,
July 1, 2000;
96(1):
126 - 131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Zamai, P. Secchiero, S. Pierpaoli, A. Bassini, S. Papa, E. S. Alnemri, L. Guidotti, M. Vitale, and G. Zauli
TNF-related apoptosis-inducing ligand (TRAIL) as a negative regulator of normal human erythropoiesis
Blood,
June 15, 2000;
95(12):
3716 - 3724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ishii, M. Nishihara, F. Ma, Y. Ebihara, K. Tsuji, S. Asano, T. Nakahata, and T. Maekawa
Expression of Stromal Cell-Derived Factor-1/Pre-B Cell Growth-Stimulating Factor Receptor, CXC Chemokine Receptor 4, on CD34+ Human Bone Marrow Cells Is a Phenotypic Alteration for Committed Lymphoid Progenitors
J. Immunol.,
October 1, 1999;
163(7):
3612 - 3620.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aiuti, L. Turchetto, M. Cota, A. Cipponi, A. Brambilla, C. Arcelloni, R. Paroni, E. Vicenzi, C. Bordignon, and G. Poli
Human CD34+ Cells Express CXCR4 and Its Ligand Stromal Cell-Derived Factor-1. Implications for Infection by T-Cell Tropic Human Immunodeficiency Virus
Blood,
July 1, 1999;
94(1):
62 - 73.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Nicholls, G. Lucas, G. J. Graham, N. H. Russell, R. Mottram, A. D. Whetton, and A.-M. Buckle
Macrophage-Inflammatory Protein-1{alpha} Receptor Expression on Normal and Chronic Myeloid Leukemia CD34+ Cells
J. Immunol.,
May 15, 1999;
162(10):
6191 - 6199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Lee, J. Ratajczak, R. W. Doms, A. M. Gewirtz, and M. Z. Ratajczak
Coreceptor/Chemokine Receptor Expression on Human Hematopoietic Cells: Biological Implications for Human Immunodeficiency Virus-Type 1 Infection
Blood,
February 15, 1999;
93(4):
1145 - 1156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shen, T. Cheng, F. I. Preffer, D. Dombkowski, M. H. Tomasson, D. E. Golan, O. Yang, W. Hofmann, J. G. Sodroski, A. D. Luster, et al.
Intrinsic Human Immunodeficiency Virus Type 1 Resistance of Hematopoietic Stem Cells Despite Coreceptor Expression
J. Virol.,
January 1, 1999;
73(1):
728 - 737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Ruiz, C. Cicala, J. Arthos, A. Kinter, A. T. Catanzaro, J. Adelsberger, K. L. Holmes, O. J. Cohen, and A. S. Fauci
Peripheral Blood-Derived CD34+ Progenitor Cells: CXC Chemokine Receptor 4 and CC Chemokine Receptor 5 Expression and Infection by HIV
J. Immunol.,
October 15, 1998;
161(8):
4169 - 4176.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. D. Berkowitz, K. P. Beckerman, T. J. Schall, and J. M. McCune
CXCR4 and CCR5 Expression Delineates Targets for HIV-1 Disruption of T Cell Differentiation
J. Immunol.,
October 1, 1998;
161(7):
3702 - 3710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Mohle, F. Bautz, S. Rafii, M. A.S. Moore, W. Brugger, and L. Kanz
The Chemokine Receptor CXCR-4 Is Expressed on CD34+ Hematopoietic Progenitors and Leukemic Cells and Mediates Transendothelial Migration Induced by Stromal Cell-Derived Factor-1
Blood,
June 15, 1998;
91(12):
4523 - 4530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Bahner, K. Kearns, S. Coutinho, E. H. Leonard, and D. B. Kohn
Infection of Human Marrow Stroma by Human Immunodeficiency Virus-1 (HIV-1) Is Both Required and Sufficient for HIV-1-Induced Hematopoietic Suppression In Vitro: Demonstration by Gene Modification of Primary Human Stroma
Blood,
September 1, 1997;
90(5):
1787 - 1798.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Deichmann, R. Kronenwett, and R. Haas
Expression of the Human Immunodeficiency Virus Type-1 Coreceptors CXCR-4 (fusin, LESTR) and CKR-5 in CD34+ Hematopoietic Progenitor Cells
Blood,
May 15, 1997;
89(10):
3522 - 3528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. O. Muench, M. G. Roncarolo, and R. Namikawa
Phenotypic and Functional Evidence for the Expression of CD4 by Hematopoietic Stem Cells Isolated From Human Fetal Liver
Blood,
February 15, 1997;
89(4):
1364 - 1375.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Venditti, G. Del Poeta, F. Buccisano, A. Tamburini, M. C. Cox, R. Stasi, A. Bruno, G. Aronica, L. Maffei, G. Suppo, et al.
Minimally Differentiated Acute Myeloid Leukemia (AML-M0): Comparison of 25 Cases With Other French-American-British Subtypes
Blood,
January 15, 1997;
89(2):
621 - 629.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
