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A novel diagnostic method of adult T-cell leukemia: monoclonal integration
of human T-cell lymphotropic virus type I provirus DNA detected by inverse
polymerase chain reaction
S Takemoto, M Matsuoka, K Yamaguchi and K Takatsuki
Second Department of Internal Medicine, Kumamoto University School of
Medicine, Japan.
Adult T-cell leukemia (ATL) is neoplasm of the mature helper T lymphocytes
and human T-cell lymphotropic virus type-I (HTLV-I) has been shown to be
causative virus of ATL. Because HTLV-I integrates its provirus randomly
into host chromosomal DNA, monoclonal integration of HTLV-I provirus
indicates the clonal proliferation of HTLV-I-infected cells. Therefore,
demonstration of clonality of HTLV-I proviral DNA is essential to diagnosis
of ATL. Southern blot analysis was used for this purpose. We developed the
novel method using inverse polymerase chain reaction (IPCR) to detect the
clonality of HTLV-I proviral DNA. This method identified the clonality in
all ATL cases. Diagnosis could be made within 3 days using this method. It
enabled us to detect specifically the presence of minimal numbers of ATL
cells with high sensitivity. It also identified the monoclonal or
oligoclonal proliferations of HTLV-I-infected cells in HTLV-I carriers and
the intermediate state, in which no clonality could be shown by
conventional Southern blot analyses. This finding indicated that even
HTLV-I carriers had monoclonal proliferation of HTLV-I-infected cells
without any symptoms. This novel method is shown to be useful for the
diagnosis of ATL and provides information on the natural course of HTLV- I
infection.
Volume 84,
Issue 9,
pp. 3080-3085,
11/01/1994
Copyright © 1994 by The American Society of Hematology
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