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Murine thrombopoietin mRNA levels are modulated by platelet count
JM McCarty, KH Sprugel, NE Fox, DE Sabath and K Kaushansky
Department of Medicine, University of Washington, Seattle, 98195-7710, USA.
The activity of the c-Mpl ligand hematopoietic progenitors meets criteria
expected for thrombopoietin (TPO). Bio-assays have shown that blood TPO
levels are inversely related to platelet mass. We sought to identify the
molecular basis for this regulation. To determine if TPO mRNA levels
respond to platelet demand, RNA from selected organs of mice with high,
normal or low platelet counts was subjected to semiquantitative reverse
transcriptase-polymerase chain reaction. Although no differences in TPO
mRNA levels between control and treated mice could be detected in liver or
kidney, TPO-specific bands were more intense after 25 to 30 polymerase
chain reaction cycles in marrow- derived mRNA from thrombocytopenic mice.
The TPO-specific bands were less intense in thrombocytotic mouse marrow and
spleen than control mouse marrow and spleen after 30 cycles. These data
support the hypothesis that TPO levels are regulated, at least in part, by
modulating mRNA levels in response to platelet demand.
Volume 86,
Issue 10,
pp. 3668-3675,
11/15/1995
Copyright © 1995 by The American Society of Hematology
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