|
|
 Previous Article | Table of Contents | Next Article 
Dysregulated expression of GATA-1 following retrovirus-mediated gene
transfer into murine hematopoietic stem cells increases erythropoiesis
SF Farina, LJ Girard, EF Vanin, AW Nienhuis and DM Bodine
Hematopoiesis Section, National Center for Human Genome Research/National
Institutes of Health, Bethesda, MD 20892-4470, USA.
Retrovirus-mediated gene transfer was used to study the effects of
dysregulated expression of the zinc-finger transcription factor, GATA- 1,
which has been shown to be required for erythropoiesis. A retroviral vector
(PGK-GATA-1) was constructed with the murine GATA-1 gene linked to the
human phosphoglycerate kinase (PGK) promoter. Expression of GATA- 1 was
demonstrated by super-shift analysis with a monoclonal antibody against
murine GATA-1 using extracts of nonerythroid cytotoxic T- lymphocyte line
(CTLL) cells transduced with the PGK-GATA-1 virus. Mouse bone marrow cells
were transduced in vitro and transplanted into recipient animals.
Polymerase chain reaction (PCR) analysis performed on DNA extracted from
peripheral blood 12 to 40 weeks posttransplantation demonstrated the
presence of the PGK-GATA-1 provirus. Proviral integrity and copy number
were demonstrated by Southern blot analysis of DNA from spleen, thymus, and
bone marrow tissues from the long-term animals. At 16 weeks posttransplant,
animals that received cells transduced by the GATA-1 virus maintained a
lower white blood cell (WBC) count and absolute neutrophil count (ANC) and
a higher red blood cell (RBC) count than control animals that received
cells transduced with a virus containing a neor gene. Erythropoiesis was
stimulated in GATA-1 and control animals by phlebotomy. GATA-1 animals
required more extensive phlebotomy to reach a hematocrit less than 25 and
their hematocrit returned to normal levels sooner than control animals. The
effect of twice-daily injections of 10 U recombinant erythropoietin (epo)
was also examined. The hematocrit of GATA-1 animals showed a more rapid and
elevated response to epo than the hematocrit of control animals. These data
suggest that dysregulated expression of GATA-1 in primitive hematopoietic
cells enlarges the pool of epo-responsive erythroid progenitor cells.
Volume 86,
Issue 11,
pp. 4124-4133,
12/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Kobayashi and M. Yamamoto
Regulation of GATA1 Gene Expression
J. Biochem.,
July 1, 2007;
142(1):
1 - 10.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Ghinassi, M. Sanchez, F. Martelli, G. Amabile, A. M. Vannucchi, G. Migliaccio, S. H. Orkin, and A. R. Migliaccio
The hypomorphic Gata1low mutation alters the proliferation/differentiation potential of the common megakaryocytic-erythroid progenitor
Blood,
February 15, 2007;
109(4):
1460 - 1471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Migliaccio, R. A. Rana, M. Sanchez, R. Lorenzini, L. Centurione, L. Bianchi, A. M. Vannucchi, G. Migliaccio, and S. H. Orkin
GATA-1 as a Regulator of Mast Cell Differentiation Revealed by the Phenotype of the GATA-1low Mouse Mutant
J. Exp. Med.,
February 3, 2003;
197(3):
281 - 296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Vannucchi, L. Bianchi, C. Cellai, F. Paoletti, R. A. Rana, R. Lorenzini, G. Migliaccio, and A. R. Migliaccio
Development of myelofibrosis in mice genetically impaired for GATA-1 expression (GATA-1low mice)
Blood,
July 30, 2002;
100(4):
1123 - 1132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. S. Strom, X. Li, J. M. Cunningham, and A. W. Nienhuis
Correction of the murine Wiskott-Aldrich syndrome phenotype by hematopoietic stem cell transplantation
Blood,
May 29, 2002;
99(12):
4626 - 4628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Vannucchi, L. Bianchi, C. Cellai, F. Paoletti, V. Carrai, A. Calzolari, L. Centurione, R. Lorenzini, C. Carta, E. Alfani, et al.
Accentuated response to phenylhydrazine and erythropoietin in mice genetically impaired for their GATA-1 expression (GATA-1low mice)
Blood,
May 15, 2001;
97(10):
3040 - 3050.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Gush, K.-L. Fu, M. Grompe, and C. E. Walsh
Phenotypic correction of Fanconi anemia group C knockout mice
Blood,
January 15, 2000;
95(2):
700 - 704.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. K. Jin, A. Takada, Y. Kon, O. Haller, and T. Watanabe
Identification of the Murine Mx2 Gene: Interferon-Induced Expression of the Mx2 Protein from the Feral Mouse Gene Confers Resistance to Vesicular Stomatitis Virus
J. Virol.,
June 1, 1999;
73(6):
4925 - 4930.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Nishiyama, K. Kiwaki, Y. Miyazaki, and T. Hasuda
Zinc and IGF-I Concentrations in Pregnant Women with Anemia before and after Supplementation with Iron and/or Zinc
J. Am. Coll. Nutr.,
June 1, 1999;
18(3):
261 - 267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Persons, J. A. Allay, E. R. Allay, R. A. Ashmun, D. Orlic, S. M. Jane, J. M. Cunningham, and A. W. Nienhuis
Enforced Expression of the GATA-2 Transcription Factor Blocks Normal Hematopoiesis
Blood,
January 15, 1999;
93(2):
488 - 499.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nishiyama, K. Irisa, T. Matsubasa, A. Higashi, and I. Matsuda
Zinc Status Relates to Hematological Deficits in Middle-Aged Women
J. Am. Coll. Nutr.,
June 1, 1998;
17(3):
291 - 295.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
