|
|
 Previous Article | Table of Contents | Next Article 
Critical involvement of transmembrane tumor necrosis factor-alpha in
endothelial programmed cell death mediated by ionizing radiation and
bacterial endotoxin
G Eissner, F Kohlhuber, M Grell, M Ueffing, P Scheurich, A Hieke, G Multhoff, GW Bornkamm and E Holler
GSF-Institut fur Klinische Molekularbiologie und Klinische Hamatologie,
Munchen, Germany.
In this report, we show that ionizing radiation (IR) at a clinically
relevant dose (4 Gy) causes apoptosis in macrovascular and microvascular
human endothelial cells. Treatment of irradiated cells with a low dose of
bacterial endotoxin (LPS), similar to the levels observed in serum during
endotoxemia, enhanced the rate of apoptosis, although LPS alone was unable
to induce programmed cell death. The cytokine and endotoxin antagonist
interleukin-10 (IL-10) reduced the rate of LPS + IR-induced apoptosis to
levels obtained with irradiation alone. Using neutralizing antibodies
against tumor necrosis factor- alpha (TNF), we could show crucial
involvement of TNF in the LPS- mediated enhancement of IR-induced
apoptosis, but not in the IR-induced apoptosis per se. However, further
analysis strongly suggested the transmembrane form of TNF (mTNF), but not
soluble TNF, to be accountable for the LPS-mediated cytotoxic effects.
Studies with anatagonistic receptor specific antibodies clearly showed that
TNF receptor type I (TR60) is essential and sufficient to elicit this
effect. These findings are of potential clinical importance because they
may disclose a relevant mechanism that leads to endothelial damage after
radiotherapy or total body irradiation used for conditioning in bone marrow
transplantation and that may thus contribute to transplant related
complications, especially in association with endotoxemia or related
inflammatory states.
Volume 86,
Issue 11,
pp. 4184-4193,
12/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
H. Zhang, D. Yan, X. Shi, H. Liang, Y. Pang, N. Qin, H. Chen, J. Wang, B. Yin, X. Jiang, et al.
Transmembrane TNF-{alpha} mediates "forward" and "reverse" signaling, inducing cell death or survival via the NF-{kappa}B pathway in Raji Burkitt lymphoma cells
J. Leukoc. Biol.,
September 1, 2008;
84(3):
789 - 797.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Rajashekhar, M. Grow, A. Willuweit, C. E. Patterson, and M. Clauss
Divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation
Physiol Genomics,
September 11, 2007;
31(1):
104 - 113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, G. G. Muramoto, A. B. Salter, S. K. Meadows, D. W. Rickman, B. Chen, H. A. Himburg, and N. J. Chao
Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice
Blood,
March 15, 2007;
109(6):
2365 - 2372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Williams, A. Verhovez, A. Milan, F. Veglio, and P. Mulatero
Protective Effect of Spironolactone on Endothelial Cell Apoptosis
Endocrinology,
May 1, 2006;
147(5):
2496 - 2505.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-X. Schmid, N. Vudattu, B. Floerchinger, M. Hilker, G. Eissner, M. Hoenicka, E. Holler, and D. E. Birnbaum
Endothelial apoptosis and circulating endothelial cells after bypass grafting with and without cardiopulmonary bypass.
Eur. J. Cardiothorac. Surg.,
April 1, 2006;
29(4):
496 - 500.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. P. O'Dea, A. J. Young, H. Yamamoto, J. L. Robotham, F. M. Brennan, and M. Takata
Lung-marginated Monocytes Modulate Pulmonary Microvascular Injury during Early Endotoxemia
Am. J. Respir. Crit. Care Med.,
November 1, 2005;
172(9):
1119 - 1127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Mezyk-Kopec, M. Bzowska, J. Potempa, M. Bzowska, N. Jura, A. Sroka, R. A. Black, and J. Bereta
Inactivation of Membrane Tumor Necrosis Factor Alpha by Gingipains from Porphyromonas gingivalis
Infect. Immun.,
March 1, 2005;
73(3):
1506 - 1514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. C. Hildebrandt, K. M. Olkiewicz, L. A. Corrion, Y. Chang, S. G. Clouthier, C. Liu, and K. R. Cooke
Donor-derived TNF-{alpha} regulates pulmonary chemokine expression and the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation
Blood,
July 15, 2004;
104(2):
586 - 593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Wong, C. Hull, R. Zhande, J. Law, and A. Karsan
Lipopolysaccharide initiates a TRAF6-mediated endothelial survival signal
Blood,
June 15, 2004;
103(12):
4520 - 4526.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ertault-Daneshpouy, C. Leboeuf, M. Lemann, F. Bouhidel, L. Ades, E. Gluckman, G. Socie, and A. Janin
Pericapillary hemorrhage as criterion of severe human digestive graft-versus-host disease
Blood,
June 15, 2004;
103(12):
4681 - 4684.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kirchner, S. Boldt, W. Kolch, S. Haffner, S. Kazak, P. Janosch, E. Holler, R. Andreesen, and G. Eissner
LPS resistance in monocytic cells caused by reverse signaling through transmembrane TNF (mTNF) is mediated by the MAPK/ERK pathway
J. Leukoc. Biol.,
February 1, 2004;
75(2):
324 - 331.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Marra, Z. X. Zhang, B. Joe, J. Campbell, G. A. Levy, J. Penninger, and L. Zhang
IL-10 Induces Regulatory T Cell Apoptosis by Up-Regulation of the Membrane Form of TNF-{alpha}
J. Immunol.,
January 15, 2004;
172(2):
1028 - 1035.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Boyle, P. L. Weissberg, and M. R. Bennett
Tumor Necrosis Factor-{alpha} Promotes Macrophage-Induced Vascular Smooth Muscle Cell Apoptosis by Direct and Autocrine Mechanisms
Arterioscler Thromb Vasc Biol,
September 1, 2003;
23(9):
1553 - 1558.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. R. Cooke, K. Olkiewicz, N. Erickson, and J. L.M. Ferrara
The role of endotoxin and the innate immune response in the pathophysiology of acute graft versus host disease
Innate Immunity,
December 1, 2002;
8(6):
441 - 448.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. Hull, G. McLean, F. Wong, P. J. Duriez, and A. Karsan
Lipopolysaccharide Signals an Endothelial Apoptosis Pathway Through TNF Receptor-Associated Factor 6-Mediated Activation of c-Jun NH2-Terminal Kinase
J. Immunol.,
September 1, 2002;
169(5):
2611 - 2618.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. S. Lew, A. Kolozsvary, S. L. Brown, and J. H. Kim
Synergistic Interaction with Arsenic Trioxide and Fractionated Radiation in Locally Advanced Murine Tumor
Cancer Res.,
August 1, 2002;
62(15):
4202 - 4205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Eissner, G. Multhoff, A. Gerbitz, S. Kirchner, S. Bauer, S. Haffner, D. Sondermann, R. Andreesen, and E. Holler
Fludarabine induces apoptosis, activation, and allogenicity in human endothelial and epithelial cells: protective effect of defibrotide
Blood,
June 17, 2002;
100(1):
334 - 340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Jarvis, U Schulz, A M Dickinson, L Sviland, G Jackson, A Konur, X N Wang, I Hromadnikova, H J Kolb, G Eissner, et al.
The detection of apoptosis in a human in vitro skin explant assay for graft versus host reactions
J. Clin. Pathol.,
February 1, 2002;
55(2):
127 - 132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Willuweit, G. Sass, A. Schoneberg, U. Eisel, G. Tiegs, and M. Clauss
Chronic Inflammation and Protection from Acute Hepatitis in Transgenic Mice Expressing TNF in Endothelial Cells
J. Immunol.,
October 1, 2001;
167(7):
3944 - 3952.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Clauss, C. Sunderkotter, B. Sveinbjornsson, S. Hippenstiel, A. Willuweit, M. Marino, E. Haas, R. Seljelid, P. Scheurich, N. Suttorp, et al.
A permissive role for tumor necrosis factor in vascular endothelial growth factor-induced vascular permeability
Blood,
March 1, 2001;
97(5):
1321 - 1329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Fine and M. G. Maguire
It Is Not Time to Abandon Radiotherapy for Neovascular Age-Related Macular Degeneration
Arch Ophthalmol,
February 1, 2001;
119(2):
275 - 276.
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Aebert, S. Kirchner, A. Keyser, D. E. Birnbaum, E. Holler, R. Andreesen, and G. Eissner
Endothelial apoptosis is induced by serum of patients after cardiopulmonary bypass
Eur. J. Cardiothorac. Surg.,
November 1, 2000;
18(5):
589 - 593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Eissner, S. Kirchner, H. Lindner, W. Kolch, P. Janosch, M. Grell, P. Scheurich, R. Andreesen, and E. Holler
Reverse Signaling Through Transmembrane TNF Confers Resistance to Lipopolysaccharide in Human Monocytes and Macrophages
J. Immunol.,
June 15, 2000;
164(12):
6193 - 6198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Iwakura, M. Fujita, K. Hasegawa, T. Sawamura, R. Nohara, S. Sasayama, and M. Komeda
Pericardial fluid from patients with unstable angina induces vascular endothelial cell apoptosis
J. Am. Coll. Cardiol.,
June 1, 2000;
35(7):
1785 - 1790.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. S. Orozco, X. Zhou, and S. G. Filler
Mechanisms of the Proinflammatory Response of Endothelial Cells to Candida albicans Infection
Infect. Immun.,
March 1, 2000;
68(3):
1134 - 1141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Stefanec
Endothelial Apoptosis: Could It Have a Role in the Pathogenesis and Treatment of Disease?
Chest,
March 1, 2000;
117(3):
841 - 854.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cavet, P. G. Middleton, M. Segall, H. Noreen, S. M. Davies, and A. M. Dickinson
Recipient Tumor Necrosis Factor-alpha and Interleukin-10 Gene Polymorphisms Associate With Early Mortality and Acute Graft-Versus-Host Disease Severity in HLA-Matched Sibling Bone Marrow Transplants
Blood,
December 1, 1999;
94(11):
3941 - 3946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Beelen, A. Elmaagacli, K.-D. Muller, H. Hirche, and U. W. Schaefer
Influence of Intestinal Bacterial Decontamination Using Metronidazole and Ciprofloxacin or Ciprofloxacin Alone on the Development of Acute Graft-Versus-Host Disease After Marrow Transplantation in Patients With Hematologic Malignancies: Final Results and Long-Term Follow-Up of an Open-Label Prospective Randomized Trial
Blood,
May 15, 1999;
93(10):
3267 - 3275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kurita-Ochiai, K. Fukushima, and K. Ochiai
Lipopolysaccharide Stimulates Butyric Acid-Induced Apoptosis in Human Peripheral Blood Mononuclear Cells
Infect. Immun.,
January 1, 1999;
67(1):
22 - 29.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Hu, E. Yee, J. M. Harlan, F. Wong, and A. Karsan
Lipopolysaccharide Induces the Antiapoptotic Molecules, A1 and A20, in Microvascular Endothelial Cells
Blood,
October 15, 1998;
92(8):
2759 - 2765.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-B. Choi, F. Wong, J. M. Harlan, P. M. Chaudhary, L. Hood, and A. Karsan
Lipopolysaccharide Mediates Endothelial Apoptosis by a FADD-dependent Pathway
J. Biol. Chem.,
August 7, 1998;
273(32):
20185 - 20188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Ksontini, S. L. D. MacKay, and L. L. Moldawer
Revisiting the Role of Tumor Necrosis Factor {alpha} and the Response to Surgical Injury and Inflammation
Arch Surg,
May 1, 1998;
133(5):
558 - 567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yen, C. A. Harrison, J. M. Devery, S. Leong, S. E. Iismaa, T. Yoshimura, and C. L. Geczy
Induction of the S100 Chemotactic Protein, CP-10, in Murine Microvascular Endothelial Cells by Proinflammatory Stimuli
Blood,
December 15, 1997;
90(12):
4812 - 4821.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Haimovitz-Friedman, C. Cordon-Cardo, S. Bayoumy, M. Garzotto, M. McLoughlin, R. Gallily, C. K. Edwards III, E. H. Schuchman, Z. Fuks, and R. Kolesnick
Lipopolysaccharide Induces Disseminated Endothelial Apoptosis Requiring Ceramide Generation
J. Exp. Med.,
December 1, 1997;
186(11):
1831 - 1841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. R. Hill, J. M. Crawford, K. R. Cooke, Y. S. Brinson, L. Pan, and J. L.M. Ferrara
Total Body Irradiation and Acute Graft-Versus-Host Disease: The Role of Gastrointestinal Damage and Inflammatory Cytokines
Blood,
October 15, 1997;
90(8):
3204 - 3213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Bombeli, A. Karsan, J. F. Tait, and J. M. Harlan
Apoptotic Vascular Endothelial Cells Become Procoagulant
Blood,
April 1, 1997;
89(7):
2429 - 2442.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Lindner, E. Holler, B. Ertl, G. Multhoff, M. Schreglmann, I. Klauke, S. Schultz-Hector, and G. Eissner
Peripheral Blood Mononuclear Cells Induce Programmed Cell Death in Human Endothelial Cells and May Prevent Repair: Role of Cytokines
Blood,
March 15, 1997;
89(6):
1931 - 1938.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Lazdins, M. Grell, M. R. Walker, K. Woods-Cook, P. Scheurich, and K. Pfizenmaier
Membrane Tumor Necrosis Factor (TNF) Induced Cooperative Signaling of TNFR60 and TNFR80 Favors Induction of Cell Death Rather Than Virus Production in HIV-infected T Cells
J. Exp. Med.,
January 6, 1997;
185(1):
81 - 90.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
