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Distinction between gamma c detection and function in YT lymphoid cells and
in the granulocyte-macrophage colony-stimulating factor-responsive human
myeloid cell line, Tf-1
NL Farner, SD Voss, TP Leary, J Gan, J Hakimi, G Evans, G Ju and PM Sondel
Department of Human Oncology, University of Wisconsin, Madison 53792, USA.
Peripheral blood monocytes respond to interleukin-2 (lL-2) and express the
gamma common (gamma c) subunit of the lL-2 receptor (lL-2R) complex.
However, the role of lL-2 in myeloid development has recently become of
interest for several reasons, including the effect gamma c mutations may or
may not have on myeloid development in patients with XSCID. Many studies of
lL-2 function in the myeloid cell lineage have been performed on a murine
background. To study gamma c expression and function in human myeloid
precursors, we introduced the human myelomonocytic cell line, Tf-1, with a
retroviral vector containing the human lL-2R beta subunit to create
functional human intermediate lL-2R consisting of beta gamma c dimers. We
have characterized this transfected variant of Tf-1 (Tf-1 beta) with regard
to its response to lL-2. Unlike the parental Tf-1 cell line that is
deficient in both lL- 2R alpha and lL-2R beta expression, the Tf-1 beta
transfectant binds and responds to lL-2 through intermediate-affinity
lL-2Rs. Scatchard analyses indicate the number of intermediate-affinity
receptors on Tf-1 beta is similar to the number found on the
well-characterized YT cell line. However, detection of gamma c on Tf-1 beta
cells is dramatically less than on YT cells by Western blot analysis and is
undetectable by flow cytometric studies and surface iodinations. The gamma
c component on YT cells is readily detected by all three methods. We
conclude from these studies that the intermediate-affinity lL-2Rs on the
Tf-1 cell line behave differently than those on YT cells with respect to
gamma c detection. Either the gamma c molecule itself is different, or the
cellular environment in which it functions is altered. Elucidation of gamma
c function on this cell line will allow for its use as a model in which
other cytokines using gamma c (including lL-2, lL-4, and lL-15) can be
studied on the same cellular background.
Volume 86,
Issue 12,
pp. 4568-4578,
12/15/1995
Copyright © 1995 by The American Society of Hematology
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