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Numerical chromosome aberrations are present within the CD30+ Hodgkin and
Reed-Sternberg cells in 100% of analyzed cases of Hodgkin's disease [see
comments]
K Weber-Matthiesen, J Deerberg, M Poetsch, W Grote and B Schlegelberger
Department of Human Genetics, University of Kiel, Germany.
In Hodgkin's disease, cytogenetically aberrant clones have been
demonstrated in a minority of cases studied. In the remaining cases, only
normal metaphases have been found, but it is questionable whether normal
karyotypes actually correspond to the pathognomonic Hodgkin and
Reed-Sternberg (HRS) cells. Numerical aberrations could be studied by
fluorescence in situ hybridization (FISH). However, in Hodgkin's disease,
the percentage of tumor cells is mostly below the detection limit of FISH,
which is near 1%. With the technique of simultaneous fluorescence
immunophenotyping and interphase cytogenetic analysis (FICTION), this
problem can be overcome. By FICTION, hybridization signals can selectively
be evaluated within the CD30a+ cell population. We have studied 30
cytogenetically analyzed cases of Hodgkin's disease by means of FICTION. In
all cases, we found numerical chromosome aberrations within the majority of
CD30+ HRS cells. In cases with complex and hyperdiploid karyotypes, the
cytogenetic results agreed with the FICTION data. There was considerable
variability in the chromosome numbers, demonstrating that karyotype
instability is an in vivo phenomenon of HRS cells. Lymphocytes never
displayed numerical chromosome changes. Our results indicate that HRS cells
regularly exhibit numerical chromosome aberrations and that the chromosome
numbers are always in the hyperploid range.
Volume 86,
Issue 4,
pp. 1464-1468,
08/15/1995
Copyright © 1995 by The American Society of Hematology
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