|
|
 Previous Article | Table of Contents | Next Article 
Localization of protein disulfide isomerase to the external surface of the
platelet plasma membrane
DW Essex, K Chen and M Swiatkowska
Department of Medicine, State University of New York, Health Science Center
at Brooklyn 11203, USA.
Protein disulfide isomerase (PDI) is an enzyme that catalyzes the formation
as well as the isomerization of disulfide bonds. In this study, antibodies
against PDI were used to show PDI antigen on the platelet surface by
indirect immunofluorescence microscopy and by flow cytometry. The platelets
were not activated, as evidenced by the absence of staining by an antibody
against P-selectin. Permeabilized platelets showed little cytosolic PDI by
indirect immunofluorescence microscopy, suggesting that the majority of
platelet PDI is localized to the platelet surface. PDI activity against
"scrambled" RNase was shown with intact platelets. The activity was
inhibited by inhibitors of PDI and by an antibody against PDI. Other blood
cells showed little PDI. Platelet surface PDI may play a role in the
various physiological and pathophysiologic processes in which platelets are
involved.
Volume 86,
Issue 6,
pp. 2168-2173,
09/15/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
H. E. van Nispen tot Pannerden, S. M. van Dijk, V. Du, and H. F. G. Heijnen
Platelet protein disulfide isomerase is localized in the dense tubular system and does not become surface expressed after activation
Blood,
November 19, 2009;
114(21):
4738 - 4740.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-C. Chen, C.-F. Lin, H.-Y. Lei, S.-C. Lin, H.-S. Liu, T.-M. Yeh, R. Anderson, and Y.-S. Lin
Deletion of the C-Terminal Region of Dengue Virus Nonstructural Protein 1 (NS1) Abolishes Anti-NS1-Mediated Platelet Dysfunction and Bleeding Tendency
J. Immunol.,
August 1, 2009;
183(3):
1797 - 1803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Walsh, D. Whelan, A. Bielanowicz, B. Skinner, R. J. Aitken, M. K. O'Bryan, and B. Nixon
Identification of the Molecular Chaperone, Heat Shock Protein 1 (Chaperonin 10), in the Reproductive Tract and in Capacitating Spermatozoa in the Male Mouse
Biol Reprod,
June 1, 2008;
78(6):
983 - 993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. H. Versteeg and W. Ruf
Tissue Factor Coagulant Function Is Enhanced by Protein-disulfide Isomerase Independent of Oxidoreductase Activity
J. Biol. Chem.,
August 31, 2007;
282(35):
25416 - 25424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Krey, H.-J. Thiel, and T. Rumenapf
Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry
J. Virol.,
April 1, 2005;
79(7):
4191 - 4200.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Jordan, J. M. Stevens, G. P. Hubbard, N. E. Barrett, T. Sage, K. S. Authi, and J. M. Gibbins
A role for the thiol isomerase protein ERP5 in platelet function
Blood,
February 15, 2005;
105(4):
1500 - 1507.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Asquith, A. J. Harman, E. A. McLaughlin, B. Nixon, and R. J. Aitken
Localization and Significance of Molecular Chaperones, Heat Shock Protein 1, and Tumor Rejection Antigen gp96 in the Male Reproductive Tract and During Capacitation and Acrosome Reaction
Biol Reprod,
February 1, 2005;
72(2):
328 - 337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Shattil and P. J. Newman
Integrins: dynamic scaffolds for adhesion and signaling in platelets
Blood,
September 15, 2004;
104(6):
1606 - 1615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Asquith, R. M. Baleato, E. A. McLaughlin, B. Nixon, and R. J. Aitken
Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition
J. Cell Sci.,
July 15, 2004;
117(16):
3645 - 3657.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lahav, K. Jurk, O. Hess, M. J. Barnes, R. W. Farndale, J. Luboshitz, and B. E. Kehrel
Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange
Blood,
September 18, 2002;
100(7):
2472 - 2478.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Chen, C. Melchior, N. H. C. Brons, N. Schlegel, J. Caen, and N. Kieffer
Probing Conformational Changes in the I-like Domain and the Cysteine-rich Repeat of Human beta 3 Integrins following Disulfide Bond Disruption by Cysteine Mutations. IDENTIFICATION OF CYSTEINE 598 INVOLVED IN alpha IIbbeta 3 ACTIVATION
J. Biol. Chem.,
October 12, 2001;
276(42):
38628 - 38635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. J. Barkalow, K. L. Barkalow, and T. N. Mayadas
Dimerization of P-selectin in platelets and endothelial cells
Blood,
November 1, 2000;
96(9):
3070 - 3077.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Bennett, B. S. Edwards, L. A. Sklar, and S. Rogelj
Sulfhydryl Regulation of L-Selectin Shedding: Phenylarsine Oxide Promotes Activation-Independent L-Selectin Shedding from Leukocytes
J. Immunol.,
April 15, 2000;
164(8):
4120 - 4129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mezghrani, J. Courageot, J. C. Mani, M. Pugniere, P. Bastiani, and R. Miquelis
Protein-disulfide Isomerase (PDI) in FRTL5 Cells. pH-DEPENDENT THYROGLOBULIN/PDI INTERACTIONS DETERMINE A NOVEL PDI FUNCTION IN THE POST-ENDOPLASMIC RETICULUM OF THYROCYTES
J. Biol. Chem.,
January 21, 2000;
275(3):
1920 - 1929.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. A. Lucero and B. Kaminer
The Role of Calcium on the Activity of ERcalcistorin/Protein-disulfide Isomerase and the Significance of the C-terminal and Its Calcium Binding. A COMPARISON WITH MAMMALIAN PROTEIN-DISULFIDE ISOMERASE
J. Biol. Chem.,
January 29, 1999;
274(5):
3243 - 3251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-M. Jiang, M. Fitzgerald, C. M. Grant, and P. J. Hogg
Redox Control of Exofacial Protein Thiols/Disulfides by Protein Disulfide Isomerase
J. Biol. Chem.,
January 22, 1999;
274(4):
2416 - 2423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Huang, T. C. Detwiler, Y. Milev, and D. W. Essex
Thiol-Disulfide Isomerization in Thrombospondin: Effects of Conformation and Protein Disulfide Isomerase
Blood,
May 1, 1997;
89(9):
3205 - 3212.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. O'Neill, A. Robinson, A. Deering, M. Ryan, D. J. Fitzgerald, and N. Moran
The Platelet Integrin alpha IIbbeta 3 Has an Endogenous Thiol Isomerase Activity
J. Biol. Chem.,
November 17, 2000;
275(47):
36984 - 36990.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Yan and J. W. Smith
A Redox Site Involved in Integrin Activation
J. Biol. Chem.,
December 15, 2000;
275(51):
39964 - 39972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Okazaki, H. Ohno, K. Takase, T. Ochiai, and T. Saito
Cell Surface Expression of Calnexin, a Molecular Chaperone in the Endoplasmic Reticulum
J. Biol. Chem.,
November 10, 2000;
275(46):
35751 - 35758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Graven, C. Molvar, J. S. Roncarati, B. D. Klahn, S. Lowrey, and H. W. Farber
Identification of protein disulfide isomerase as an endothelial hypoxic stress protein
Am J Physiol Lung Cell Mol Physiol,
May 1, 2002;
282(5):
L996 - L1003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
