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Antibody ligation of CD9 modifies production of myeloid cells in long- term
cultures
K Oritani, X Wu, K Medina, J Hudson, K Miyake, JM Gimble, SA Burstein and PW Kincade
Immunobiology and Cancer Program, Oklahoma Medical Research Foundation,
Oklahoma, City, OK USA.
The KMC8.8 monoclonal antibody was made by immunizing rats with the BMS2
stromal cell clone, and was selected for further study because its ability
to inhibit production of myeloid cells in Dexter cultures but not that of
lymphoid cells in Whitlock-Witte cultures. The influence on myeloid
progenitors might have been indirect, since the antibody did not prevent
responsiveness to colony-stimulating factors in semisolid agar cultures.
Furthermore, there was no inhibition, and some augmentation, of cell
production when the antibody was added to established Dexter cultures. A
cDNA clone that encoded the KMC8.8- recognized molecule was isolated by
expression cloning and found to be identical in sequence to a previously
published murine CD9 homologue. The antibody and cDNA clone were used to
establish that CD9 is expressed by stromal cells, megakaryocytes,
platelets, myeloid cells, and subpopulations of mature lymphocytes in mice.
Treatment with the KMC8.8/CD9 antibody slightly augmented adhesion between
myeloid cells and stromal cells, consistent with previous reports that this
member of the tetraspan family of proteins can transmit proadhesive signals
to human platelets and lymphoid cells. CD9 might participate in cell-cell
interactions critical for correct orientation and movement of maturing
myeloid cells in bone marrow.
Volume 87,
Issue 6,
pp. 2252-2261,
03/15/1996
Copyright © 1996 by The American Society of Hematology
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