|
|
 Previous Article | Table of Contents | Next Article 
Characterization of RAFTK, a novel focal adhesion kinase, and its
integrin-dependent phosphorylation and activation in megakaryocytes
J Li, H Avraham, RA Rogers, S Raja and S Avraham
Division of Hematology/Oncology, Deaconess Hospital, Department of
Medicine, Harvard Medical School, Boston, MA 02215, USA.
We have recently isolated a cDNA encoding a novel human intracellular
tyrosine kinase, termed RAFTK (for a related adhesion focal tyrosine
kinase). The RAFTK cDNA, which encodes a polypeptide of 1,009 amino acids,
shares 65% homology to the focal adhesion kinase (FAK), including several
consensus motifs. In this report, we describe the biochemical
characterization and functional analysis of the RAFTK protein. Coexpression
of RAFTK and FAK proteins in megakaryocytic cells and blood platelets was
observed. Using a specific antibody to RAFTK and the monoclonal antibody
2A7 to FAK, FAK and RAFTK could be distinguished antigenically. RAFTK had
intrinsic tyrosine kinase and autokinase activities. It was phosphorylated
on tyrosine in growing cultures of COS cells transfected with the
pCDNAIII/flag-RAFTK expression vector containing the RAFTK cDNA ligated
with the 8 amino acid flag peptide sequence. Similar to FAK,
dephosphorylation of RAFTK was observed when adherent transfected COS cells
were detached. Phosphorylation was regained upon replating of these cells
on the fibronectincoated dishes. Analysis of tyrosine-phosphorylated RAFTK
from adherent transfected COS cells showed that the Src homology 2 (SH2)
domains of the Src and Fyn protein kinases as well as the Grb2 adaptor
protein were able to specifically associate with RAFTK. Tyrosine
phosphorylation of endogenous RAFTK was observed upon fibronectin-induced
activation of human megakaryocytic cells. Furthermore, colocalization of
RAFTK protein with vinculin, a focal adhesion protein, was observed by
confocal microscopy in focal adhesion- like structures in adherent CMK
cells and in transfected pCDNAIII/flag- RAFTK COS cells upon fibronectin
activation. These data suggest that RAFTK is a novel member of the FAK
family, that it localizes to focal adhesion-like structures in CMK
megakaryocytic cells, that it participates in integrinmediated signaling
pathways in megakaryocytes, and that it is able to associate with the
tyrosine kinases Src and Fyn as well as the adaptor protein Grb2 via
SH2-phosphotyrosine interactions.
Volume 88,
Issue 2,
pp. 417-428,
07/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Bunda, Y. Wang, T. F. Mitts, P. Liu, S. Arab, M. Arabkhari, and A. Hinek
Aldosterone Stimulates Elastogenesis in Cardiac Fibroblasts via Mineralocorticoid Receptor-independent Action Involving the Consecutive Activation of G{alpha}13, c-Src, the Insulin-like Growth Factor-I Receptor, and Phosphatidylinositol 3-Kinase/Akt
J. Biol. Chem.,
June 12, 2009;
284(24):
16633 - 16647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. S. Hitchcock, N. E. Fox, N. Prevost, K. Sear, S. J. Shattil, and K. Kaushansky
Roles of focal adhesion kinase (FAK) in megakaryopoiesis and platelet function: studies using a megakaryocyte lineage specific FAK knockout
Blood,
January 15, 2008;
111(2):
596 - 604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Moro, S. Reineri, D. Piranda, D. Pietrapiana, P. Lova, A. Bertoni, A. Graziani, P. Defilippi, I. Canobbio, M. Torti, et al.
Nongenomic effects of 17{beta}-estradiol in human platelets: potentiation of thrombin-induced aggregation through estrogen receptor {beta} and Src kinase
Blood,
January 1, 2005;
105(1):
115 - 121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Y. Park, H. K. Avraham, and S. Avraham
RAFTK/Pyk2 Activation Is Mediated by Trans-acting Autophosphorylation in a Src-independent Manner
J. Biol. Chem.,
August 6, 2004;
279(32):
33315 - 33322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Boutahar, A. Guignandon, L. Vico, and M.-H. Lafage-Proust
Mechanical Strain on Osteoblasts Activates Autophosphorylation of Focal Adhesion Kinase and Proline-rich Tyrosine Kinase 2 Tyrosine Sites Involved in ERK Activation
J. Biol. Chem.,
July 16, 2004;
279(29):
30588 - 30599.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. N. Meyer, R. F. Gastwirt, D. D. Schlaepfer, and D. J. Donoghue
The Cytoplasmic Tyrosine Kinase Pyk2 as a Novel Effector of Fibroblast Growth Factor Receptor 3 Activation
J. Biol. Chem.,
July 2, 2004;
279(27):
28450 - 28457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Taniyama, D. S. Weber, P. Rocic, L. Hilenski, M. L. Akers, J. Park, B. A. Hemmings, R. W. Alexander, and K. K. Griendling
Pyk2- and Src-Dependent Tyrosine Phosphorylation of PDK1 Regulates Focal Adhesions
Mol. Cell. Biol.,
November 15, 2003;
23(22):
8019 - 8029.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Takahashi, H. Yamashita, Y. Nagano, T. Nakamura, H. Ohmori, H. Avraham, S. Avraham, M. Yasuda, and M. Matsumoto
Identification and Characterization of a Novel Pyk2/Related Adhesion Focal Tyrosine Kinase-associated Protein That Inhibits {alpha}-Synuclein Phosphorylation
J. Biol. Chem.,
October 24, 2003;
278(43):
42225 - 42233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. K. Avraham, T.-H. Lee, Y. Koh, T.-A. Kim, S. Jiang, M. Sussman, A. M. Samarel, and S. Avraham
Vascular Endothelial Growth Factor Regulates Focal Adhesion Assembly in Human Brain Microvascular Endothelial Cells through Activation of the Focal Adhesion Kinase and Related Adhesion Focal Tyrosine Kinase
J. Biol. Chem.,
September 19, 2003;
278(38):
36661 - 36668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-J. Cheng, Y.-J. Chao, and D. L. Wang
Cyclic Strain Activates Redox-sensitive Proline-rich Tyrosine Kinase 2 (PYK2) in Endothelial Cells
J. Biol. Chem.,
December 6, 2002;
277(50):
48152 - 48157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Dunty and M. D. Schaller
The N Termini of Focal Adhesion Kinase Family Members Regulate Substrate Phosphorylation, Localization, and Cell Morphology
J. Biol. Chem.,
November 15, 2002;
277(47):
45644 - 45654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Seta, Z. Spicer, Y. Yuan, G. Lu, and D. E. Millhorn
Responding to Hypoxia: Lessons From a Model Cell Line
Sci. Signal.,
August 20, 2002;
2002(146):
re11 - re11.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang, Y. Yang, X. Guo, E. R. Sampson, C.-L. Hsu, M.-Y. Tsai, S. Yeh, G. Wu, Y. Guo, and C. Chang
Suppression of Androgen Receptor Transactivation by Pyk2 via Interaction and Phosphorylation of the ARA55 Coregulator
J. Biol. Chem.,
May 3, 2002;
277(18):
15426 - 15431.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q.-S. Du, X.-R. Ren, Y. Xie, Q. Wang, L. Mei, and W.-C. Xiong
Inhibition of PYK2-induced actin cytoskeleton reorganization, PYK2 autophosphorylation and focal adhesion targeting by FAK
J. Cell Sci.,
March 10, 2002;
114(16):
2977 - 2987.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Rodriguez-Fernandez, L. Sanchez-Martin, C. A. de Frutos, D. Sancho, M. Robinson, F. Sanchez-Madrid, and C. Cabanas
LFA-1 integrin and the microtubular cytoskeleton are involved in the Ca2+-mediated regulation of the activity of the tyrosine kinase PYK2 in T cells
J. Leukoc. Biol.,
March 1, 2002;
71(3):
520 - 530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Podar, Y.-T. Tai, B. K. Lin, R. P. Narsimhan, M. Sattler, T. Kijima, R. Salgia, D. Gupta, D. Chauhan, and K. C. Anderson
Vascular Endothelial Growth Factor-induced Migration of Multiple Myeloma Cells Is Associated with beta 1 Integrin- and Phosphatidylinositol 3-Kinase-dependent PKCalpha Activation
J. Biol. Chem.,
March 1, 2002;
277(10):
7875 - 7881.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Bruce-Staskal, C. L. Weidow, J. J. Gibson, and A. H. Bouton
Cas, Fak and Pyk2 function in diverse signaling cascades to promote Yersinia uptake
J. Cell Sci.,
January 7, 2002;
115(13):
2689 - 2700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-F. Zhang, J.-F. Wang, E. Matczak, J. Proper, and J. E. Groopman
Janus kinase 2 is involved in stromal cell-derived factor-1{alpha}-induced tyrosine phosphorylation of focal adhesion proteins and migration of hematopoietic progenitor cells
Blood,
June 1, 2001;
97(11):
3342 - 3348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Cooley, J. M. Broome, C. Ohngemach, L. H. Romer, and M. D. Schaller
Paxillin Binding Is Not the Sole Determinant of Focal Adhesion Localization or Dominant-Negative Activity of Focal Adhesion Kinase/Focal Adhesion Kinase-related Nonkinase
Mol. Biol. Cell,
September 1, 2000;
11(9):
3247 - 3263.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Miura, Y. Tohyama, T. Hishita, A. Lala, E. De Nardin, Y. Yoshida, H. Yamamura, T. Uchiyama, and K. Tohyama
Pyk2 and Syk participate in functional activation of granulocytic HL-60 cells in a different manner
Blood,
September 1, 2000;
96(5):
1733 - 1739.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Sancho, M. Nieto, M. Llano, J. L. Rodriguez-Fernandez, R. Tejedor, S. Avraham, C. Cabanas, M. Lopez-Botet, and F. Sanchez-Madrid
The Tyrosine Kinase Pyk-2/Raftk Regulates Natural Killer (Nk) Cell Cytotoxic Response, and Is Translocated and Activated upon Specific Target Cell Recognition and Killing
J. Cell Biol.,
June 12, 2000;
149(6):
1249 - 1262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Wang, I.-W. Park, and J. E. Groopman
Stromal cell-derived factor-1alpha stimulates tyrosine phosphorylation of multiple focal adhesion proteins and induces migration of hematopoietic progenitor cells: roles of phosphoinositide-3 kinase and protein kinase C
Blood,
April 15, 2000;
95(8):
2505 - 2513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Benbernou, K. Muegge, and S. K. Durum
Interleukin (IL)-7 Induces Rapid Activation of Pyk2, Which Is Bound to Janus Kinase 1 and IL-7Ralpha
J. Biol. Chem.,
March 15, 2000;
275(10):
7060 - 7065.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Williams and A. J. Ridley
Lipopolysaccharide Induces Actin Reorganization and Tyrosine Phosphorylation of Pyk2 and Paxillin in Monocytes and Macrophages
J. Immunol.,
February 15, 2000;
164(4):
2028 - 2036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Tsuchida, E. R. Manthei, T. Alam, S. J. Knechtle, and M. M. Hamawy
Regulation of T Cell Receptor- and CD28-induced Tyrosine Phosphorylation of the Focal Adhesion Tyrosine Kinases Pyk2 and Fak by Protein Kinase C. A ROLE FOR PROTEIN TYROSINE PHOSPHATASES
J. Biol. Chem.,
January 14, 2000;
275(2):
1344 - 1350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Tsuchida, E. R. Manthei, T. Alam, S. J. Knechtle, and M. M. Hamawy
T Cell Activation Up-Regulates the Expression of the Focal Adhesion Kinase Pyk2: Opposing Roles for the Activation of Protein Kinase C and the Increase in Intracellular Ca2+
J. Immunol.,
December 15, 1999;
163(12):
6640 - 6650.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. H. Palmer, L. I. Fessler, P. T. Edeen, S. J. Madigan, M. McKeown, and T. Hunter
DFak56 Is a Novel Drosophila melanogaster Focal Adhesion Kinase
J. Biol. Chem.,
December 10, 1999;
274(50):
35621 - 35629.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Munshi, R. K. Ganju, S. Avraham, E. A. Mesri, and J. E. Groopman
Kaposi's Sarcoma-associated Herpesvirus-encoded G Protein-coupled Receptor Activation of c-Jun Amino-terminal Kinase/Stress-activated Protein Kinase and Lyn Kinase Is Mediated by Related Adhesion Focal Tyrosine Kinase/Proline-rich Tyrosine Kinase 2
J. Biol. Chem.,
November 5, 1999;
274(45):
31863 - 31867.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Tapia, H. A. Ferris, R. T. Jensen, and L. J. Garcia
Cholecystokinin Activates PYK2/CAKbeta by a Phospholipase C-dependent Mechanism and Its Association with the Mitogen-activated Protein Kinase Signaling Pathway in Pancreatic Acinar Cells
J. Biol. Chem.,
October 29, 1999;
274(44):
31261 - 31271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kumar, S. Avraham, A. Bharti, J. Goyal, P. Pandey, and S. Kharbanda
Negative Regulation of PYK2/Related Adhesion Focal Tyrosine Kinase Signal Transduction by Hematopoietic Tyrosine Phosphatase SHPTP1
J. Biol. Chem.,
October 22, 1999;
274(43):
30657 - 30663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Shen and M. D. Schaller
Focal Adhesion Targeting: The Critical Determinant of FAK Regulation and Substrate Phosphorylation
Mol. Biol. Cell,
August 1, 1999;
10(8):
2507 - 2518.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Taniguchi, R. London, K. Schinkmann, S. Jiang, and H. Avraham
The Receptor Protein Tyrosine Phosphatase, PTP-RO, Is Upregulated During Megakaryocyte Differentiation and Is Associated With the c-Kit Receptor
Blood,
July 15, 1999;
94(2):
539 - 549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Owen, P. J. Ruest, D. W. Fry, and S. K. Hanks
Induced Focal Adhesion Kinase (FAK) Expression in FAK-Null Cells Enhances Cell Spreading and Migration Requiring Both Auto- and Activation Loop Phosphorylation Sites and Inhibits Adhesion-Dependent Tyrosine Phosphorylation of Pyk2
Mol. Cell. Biol.,
July 1, 1999;
19(7):
4806 - 4818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Soltysik-Espanola, R. A. Rogers, S. Jiang, T.-A. Kim, R. Gaedigk, R. A. White, H. Avraham, and S. Avraham
Characterization of Mayven, a Novel Actin-binding Protein Predominantly Expressed in Brain
Mol. Biol. Cell,
July 1, 1999;
10(7):
2361 - 2375.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. L. Rodríguez-Fernández, M. Gómez, A. Luque, N. Hogg, F. Sánchez-Madrid, and C. Cabañas
The Interaction of Activated Integrin Lymphocyte Function-associated Antigen 1 with Ligand Intercellular Adhesion Molecule 1 Induces Activation and Redistribution of Focal Adhesion Kinase and Proline-rich Tyrosine Kinase 2 in T Lymphocytes
Mol. Biol. Cell,
June 1, 1999;
10(6):
1891 - 1907.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
X. Li, R. C. Dy, W. G. Cance, L. M. Graves, and H. S. Earp
Interactions between Two Cytoskeleton-associated Tyrosine Kinases: Calcium-dependent Tyrosine Kinase and Focal Adhesion Tyrosine Kinase
J. Biol. Chem.,
March 26, 1999;
274(13):
8917 - 8924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Tsuchida, S. J. Knechtle, and M. M. Hamawy
CD28 Ligation Induces Tyrosine Phosphorylation of Pyk2 but Not Fak in Jurkat T Cells
J. Biol. Chem.,
March 5, 1999;
274(10):
6735 - 6740.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Andreev, J.-P. Simon, D. D. Sabatini, J. Kam, G. Plowman, P. A. Randazzo, and J. Schlessinger
Identification of a New Pyk2 Target Protein with Arf-GAP Activity
Mol. Cell. Biol.,
March 1, 1999;
19(3):
2338 - 2350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Suen, D Ilic, E Caveggion, G Berton, C. Damsky, and C. Lowell
Impaired integrin-mediated signal transduction, altered cytoskeletal structure and reduced motility in Hck/Fgr deficient macrophages
J. Cell Sci.,
January 11, 1999;
112(22):
4067 - 4078.
[Abstract]
[PDF]
|
|
|
|
|
|
|
F. Mainiero, A. Gismondi, A. Soriani, M. Cippitelli, G. Palmieri, J. Jacobelli, M. Piccoli, L. Frati, and A. Santoni
Integrin-mediated Ras-Extracellular Regulated Kinase (ERK) Signaling Regulates Interferon {gamma} Production in Human Natural Killer Cells
J. Exp. Med.,
October 5, 1998;
188(7):
1267 - 1275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Dutt, J.-F. Wang, and J. E. Groopman
Stromal Cell-Derived Factor-1{alpha} and Stem Cell Factor/kit Ligand Share Signaling Pathways in Hemopoietic Progenitors: A Potential Mechanism for Cooperative Induction of Chemotaxis
J. Immunol.,
October 1, 1998;
161(7):
3652 - 3658.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Soltoff
Related Adhesion Focal Tyrosine Kinase and the Epidermal Growth Factor Receptor Mediate the Stimulation of Mitogen-activated Protein Kinase by the G-protein-coupled P2Y2 Receptor. PHORBOL ESTER OR [Ca2+]i ELEVATION CAN SUBSTITUTE FOR RECEPTOR ACTIVATION
J. Biol. Chem.,
September 4, 1998;
273(36):
23110 - 23117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Ganju, S. A. Brubaker, J. Meyer, P. Dutt, Y. Yang, S. Qin, W. Newman, and J. E. Groopman
The alpha -Chemokine, Stromal Cell-derived Factor-1alpha , Binds to the Transmembrane G-protein-coupled CXCR-4 Receptor and Activates Multiple Signal Transduction Pathways
J. Biol. Chem.,
September 4, 1998;
273(36):
23169 - 23175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Zhang, H. Avraham, and D. M. Cohen
Urea and NaCl differentially regulate FAK and RAFTK/PYK2 in mIMCD3 renal medullary cells
Am J Physiol Renal Physiol,
September 1, 1998;
275(3):
F447 - F451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Ganju, N. Munshi, B. C. Nair, Z.-Y. Liu, P. Gill, and J. E. Groopman
Human Immunodeficiency Virus Tat Modulates the Flk-1/KDR Receptor, Mitogen-Activated Protein Kinases, and Components of Focal Adhesion in Kaposi's Sarcoma Cells
J. Virol.,
July 1, 1998;
72(7):
6131 - 6137.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Dikic, I. Dikic, and J. Schlessinger
Identification of a New Pyk2 Isoform Implicated in Chemokine and Antigen Receptor Signaling
J. Biol. Chem.,
June 5, 1998;
273(23):
14301 - 14308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Aplin, A. Howe, S. K. Alahari, and R. L. Juliano
Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins
Pharmacol. Rev.,
June 1, 1998;
50(2):
197 - 264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. C. Hatch, R. K. Ganju, D. Hiregowdara, S. Avraham, and J. E. Groopman
The Related Adhesion Focal Tyrosine Kinase (RAFTK) Is Tyrosine Phosphorylated and Participates in Colony-Stimulating Factor-1/Macrophage Colony-Stimulating Factor Signaling in Monocyte-Macrophages
Blood,
May 15, 1998;
91(10):
3967 - 3973.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T.-A. Kim, J. Lim, S. Ota, S. Raja, R. Rogers, B. Rivnay, H. Avraham, and S. Avraham
NRP/B, a Novel Nuclear Matrix Protein, Associates With p110RB and Is Involved in Neuronal Differentiation
J. Cell Biol.,
May 4, 1998;
141(3):
553 - 566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Jeschke, G. J. R. Standke, and M. Susa
Fluoroaluminate Induces Activation and Association of Src and Pyk2 Tyrosine Kinases in Osteoblastic MC3T3-E1 Cells
J. Biol. Chem.,
May 1, 1998;
273(18):
11354 - 11361.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Felsch, T. G. Cachero, and E. G. Peralta
Activation of protein tyrosine kinase PYK2 by the m1 muscarinic acetylcholine receptor
PNAS,
April 28, 1998;
95(9):
5051 - 5056.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zrihan-Licht, B. Deng, Y. Yarden, G. McShan, I. Keydar, and H. Avraham
Csk Homologous Kinase, a Novel Signaling Molecule, Directly Associates with the Activated ErbB-2 Receptor in Breast Cancer Cells and Inhibits Their Proliferation
J. Biol. Chem.,
February 13, 1998;
273(7):
4065 - 4072.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Ganju, P. Dutt, L. Wu, W. Newman, H. Avraham, S. Avraham, and J. E. Groopman
beta -Chemokine Receptor CCR5 Signals Via the Novel Tyrosine Kinase RAFTK
Blood,
February 1, 1998;
91(3):
791 - 797.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Soltoff, H. Avraham, S. Avraham, and L. C. Cantley
Activation of P2Y2 Receptors by UTP and ATP Stimulates Mitogen-activated Kinase Activity through a Pathway That Involves Related Adhesion Focal Tyrosine Kinase and Protein Kinase C
J. Biol. Chem.,
January 30, 1998;
273(5):
2653 - 2660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Zheng, Z. Xing, Z. C. Bian, C. Guo, A. Akbay, L. Warner, and J.-L. Guan
Differential Regulation of Pyk2 and Focal Adhesion Kinase (FAK). THE C-TERMINAL DOMAIN OF FAK CONFERS RESPONSE TO CELL ADHESION
J. Biol. Chem.,
January 23, 1998;
273(4):
2384 - 2389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Brinson, T. Harding, P. A. Diliberto, Y. He, X. Li, D. Hunter, B. Herman, H. S. Earp, and L. M. Graves
Regulation of a Calcium-dependent Tyrosine Kinase in Vascular Smooth Muscle Cells by Angiotensin II and Platelet-derived Growth Factor. DEPENDENCE ON CALCIUM AND THE ACTIN CYTOSKELETON
J. Biol. Chem.,
January 16, 1998;
273(3):
1711 - 1718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Matsuya, H. Sasaki, H. Aoto, T. Mitaka, K. Nagura, T. Ohba, M. Ishino, S. Takahashi, R. Suzuki, and T. Sasaki
Cell Adhesion Kinase beta Forms a Complex with a New Member, Hic-5, of Proteins Localized at Focal Adhesions
J. Biol. Chem.,
January 9, 1998;
273(2):
1003 - 1014.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Okazaki, J. Zhang, M. M. Hamawy, and R. P. Siraganian
Activation of Protein-tyrosine Kinase Pyk2 Is Downstream of Syk in Fcepsilon RI Signaling
J. Biol. Chem.,
December 19, 1997;
272(51):
32443 - 32447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Wang, R. K. Ganju, Z.-Y. Liu, H. Avraham, S. Avraham, and J. E. Groopman
Signal Transduction in Human Hematopoietic Cells by Vascular Endothelial Growth Factor Related Protein, a Novel Ligand for the FLT4 Receptor
Blood,
November 1, 1997;
90(9):
3507 - 3515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Schaller and T. Sasaki
Differential Signaling by the Focal Adhesion Kinase and Cell Adhesion Kinase beta
J. Biol. Chem.,
October 3, 1997;
272(40):
25319 - 25325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-Y. Liu, R. K. Ganju, J.-F. Wang, K. Schweitzer, B. Weksler, S. Avraham, and J. E. Groopman
Characterization of Signal Transduction Pathways in Human Bone Marrow Endothelial Cells
Blood,
September 15, 1997;
90(6):
2253 - 2259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Astier, S. N. Manie, H. Avraham, H. Hirai, S. F. Law, Y. Zhang, E. A. Golemis, Y. Fu, B. J. Druker, N. Haghayeghi, et al.
The Related Adhesion Focal Tyrosine Kinase Differentially Phosphorylates p130Cas and the Cas-like Protein, p105HEF1
J. Biol. Chem.,
August 8, 1997;
272(32):
19719 - 19724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sattler, R. Salgia, G. Shrikhande, S. Verma, N. Uemura, S. F. Law, E. A. Golemis, and J. D. Griffin
Differential Signaling after beta 1 Integrin Ligation Is Mediated Through Binding of CRKL to p120CBL and p110HEF1
J. Biol. Chem.,
May 30, 1997;
272(22):
14320 - 14326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Li and H. S. Earp
Paxillin Is Tyrosine-phosphorylated by and Preferentially Associates with the Calcium-dependent Tyrosine Kinase in Rat Liver Epithelial Cells
J. Biol. Chem.,
May 30, 1997;
272(22):
14341 - 14348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. D. Schlaepfer and T. Hunter
Focal Adhesion Kinase Overexpression Enhances Ras-dependent Integrin Signaling to ERK2/Mitogen-activated Protein Kinase through Interactions with and Activation of c-Src
J. Biol. Chem.,
May 16, 1997;
272(20):
13189 - 13195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Hiregowdara, H. Avraham, Y. Fu, R. London, and S. Avraham
Tyrosine Phosphorylation of the Related Adhesion Focal Tyrosine Kinase in Megakaryocytes upon Stem Cell Factor and Phorbol Myristate Acetate Stimulation and Its Association with Paxillin
J. Biol. Chem.,
April 18, 1997;
272(16):
10804 - 10810.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Raja, S. Avraham, and H. Avraham
Tyrosine Phosphorylation of the Novel Protein-tyrosine Kinase RAFTK during an Early Phase of Platelet Activation by an Integrin Glycoprotein IIb-IIIa-independent Mechanism
J. Biol. Chem.,
April 18, 1997;
272(16):
10941 - 10947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Ganju, W. C. Hatch, H. Avraham, M. A. Ona, B. Druker, S. Avraham, and J. E. Groopman
RAFTK, a Novel Member of the Focal Adhesion Kinase Family, Is Phosphorylated and Associates with Signaling Molecules upon Activation of Mature T Lymphocytes
J. Exp. Med.,
March 17, 1997;
185(6):
1055 - 1064.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Liu, P. Oh, T. Horner, R. A. Rogers, and J. E. Schnitzer
Organized Endothelial Cell Surface Signal Transduction in Caveolae Distinct from Glycosylphosphatidylinositol-anchored Protein Microdomains
J. Biol. Chem.,
March 14, 1997;
272(11):
7211 - 7222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Astier, H. Avraham, S. N. Manie, J. Groopman, T. Canty, S. Avraham, and A. S. Freedman
The Related Adhesion Focal Tyrosine Kinase Is Tyrosine-phosphorylated after beta 1-Integrin Stimulation in B Cells and Binds to p130cas
J. Biol. Chem.,
January 3, 1997;
272(1):
228 - 232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D Ilic, C. Damsky, and T Yamamoto
Focal adhesion kinase: at the crossroads of signal transduction
J. Cell Sci.,
January 2, 1997;
110(4):
401 - 407.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R. Salgia, S. Avraham, E. Pisick, J.-L. Li, S. Raja, E. A. Greenfield, M. Sattler, H. Avraham, and J. D. Griffin
The Related Adhesion Focal Tyrosine Kinase Forms a Complex with Paxillin in Hematopoietic Cells
J. Biol. Chem.,
December 6, 1996;
271(49):
31222 - 31226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Ganju, S. A. Brubaker, R. D. Chernock, S. Avraham, and J. E. Groopman
beta -Chemokine Receptor CCR5 Signals through SHP1, SHP2, and Syk
J. Biol. Chem.,
June 2, 2000;
275(23):
17263 - 17268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Watson, T. W. Harding, V. Golubovskaya, J. S. Morris, D. Hunter, X. Li, J. S. Haskill, and H. S. Earp
Inhibition of the Calcium-dependent Tyrosine Kinase (CADTK) Blocks Monocyte Spreading and Motility
J. Biol. Chem.,
January 26, 2001;
276(5):
3536 - 3542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Anfosso, N. Bardin, E. Vivier, F. Sabatier, J. Sampol, and F. Dignat-George
Outside-in Signaling Pathway Linked to CD146 Engagement in Human Endothelial Cells
J. Biol. Chem.,
January 5, 2001;
276(2):
1564 - 1569.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. T. Duong, I. Nakamura, P. T. Lakkakorpi, L. Lipfert, A. J. Bett, and G. A. Rodan
Inhibition of Osteoclast Function by Adenovirus Expressing Antisense Protein-tyrosine Kinase 2
J. Biol. Chem.,
March 2, 2001;
276(10):
7484 - 7492.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Lyons, J. M. Dunty, E. M. Schaefer, and M. D. Schaller
Inhibition of the Catalytic Activity of Cell Adhesion Kinase beta by Protein-tyrosine Phosphatase-PEST-mediated Dephosphorylation
J. Biol. Chem.,
June 22, 2001;
276(26):
24422 - 24431.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Y. Park, H. Avraham, and S. Avraham
Characterization of the Tyrosine Kinases RAFTK/Pyk2 and FAK in Nerve Growth Factor-induced Neuronal Differentiation
J. Biol. Chem.,
June 23, 2000;
275(26):
19768 - 19777.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
