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Modulation of erythrocyte potassium chloride cotransport, potassium
content, and density by dietary magnesium intake in transgenic SAD mouse
L De Franceschi, Y Beuzard, H Jouault and C Brugnara
Department of Internal Medicine, University of Verona, Italy.
Prevention of erythrocyte dehydration is a potential therapeutic strategy
for sickle cell disease. Increasing erythrocyte magnesium (Mg) could
inhibit sickle cell dehydration by increasing chloride (CI) and water
content and by inhibiting potassium chloride (K-CI) cotransport. In
transgenic SAD 1 and (control) C57BL/6 normal mice, we investigated the
effect of 2 weeks of diet with either low Mg (6 +/- 2 mg/kg body weight/d)
or high Mg (1,000 +/- 20 mg/kg body weight/ d), in comparison with a diet
of standard Mg (400 +/- 20 mg/ kg body weight/d). The high- Mg diet
increased SAD 1 erythrocyte Mg and K contents and reduced K-CI cotransport
activity, mean corpuscular hemoglobin concentration (MCHC), cell density,
and reticulocyte count. SAD 1 mice treated with low-Mg diet showed a
significant reduction in erythrocyte Mg and K contents and increases in
K-CI cotransport, MCHC, cell density, and reticulocyte counts. In SAD 1
mice, hematocrit (Hct) and hemoglobin (Hb) decreased significantly with low
Mg diet and increased significantly with high-Mg diet. The C57BL/6 controls
showed significant changes only in erythrocyte Mg and K content, and K-CI
cotransport activities, similar to those observed in SAD 1 mice. Thus, in
the SAD 1 mouse, changes in dietary Mg modulate K-CI cotransport, modify
erythrocyte dehydration, and ultimately affect Hb levels.
Volume 88,
Issue 7,
pp. 2738-2744,
10/01/1996
Copyright © 1996 by The American Society of Hematology
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