|
|
 Previous Article | Table of Contents | Next Article 
Localization and regulation of thrombopoietin mRNa expression in human
kidney, liver, bone marrow, and spleen using in situ hybridization
R Sungaran, B Markovic and BH Chong
Department of Haematology, Prince of Wales Hospital/school of Pathology,
Sydney, Australia.
Thrombopoietin (TPO) is the primary hematopoietic growth factor involved in
the regulation of platelet production. Although the kidney, liver, bone
marrow (BM), and spleen have been identified as the major sources of TPO
production, the precise cellular location of TPO mRNA expression in these
tissues remains unknown. We have identified the cells expressing TPO mRNA
in the human kidney, liver, and BM using an in situ hybridization assay. In
the BM of individuals with normal platelet counts, the hybridization signal
was too weak to allow identification of the TPO mRNA expressing cells.
However, in thrombocytopenic subjects with aplastic anemia,
postchemotherapy marrow aplasia, and immune thrombocytopenia, the stromal
cells showed strong TPO mRNA expression. In the human subjects with normal
platelet counts, the cells of the proximal convoluted tubules of the kidney
showed consistent positive staining whereas the signal in the cells of the
distal convoluted tubules was less consistent. Strong hybridization signal
was also evident in the hepatocytes. The hybridization signal in the
spleen, even in thrombocytopenic subjects, was too weak to allow confident
identification of the cells expressing TPO mRNA. In all subjects, the
interstitial cells and endothelial cells of the liver and spleen, the renal
peritubular cells, and the hematopoietic precursor cells of the BM showed
no TPO mRNA expression. Our data suggest that TPO mRNA expression in the
human BM may be modulated by platelet mass.
Volume 89,
Issue 1,
pp. 101-107,
01/01/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Dordelmann, R. Telgmann, E. Brand, C. Hagedorn, B. Schroer, S. Hasenkamp, P. Baumgart, P. Kleine-Katthofer, M. Paul, and S.-M. Brand-Herrmann
Functional and Structural Profiling of the Human Thrombopoietin Gene Promoter
J. Biol. Chem.,
September 5, 2008;
283(36):
24382 - 24391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kaushansky
Historical review: megakaryopoiesis and thrombopoiesis
Blood,
February 1, 2008;
111(3):
981 - 986.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kaushansky
Lineage-specific hematopoietic growth factors.
N. Engl. J. Med.,
May 11, 2006;
354(19):
2034 - 2045.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Romanelli, I. Petrai, G. Robino, E. Efsen, E. Novo, A. Bonacchi, G. Pagliai, A. Grossi, M. Parola, N. Navari, et al.
Thrombopoietin stimulates migration and activates multiple signaling pathways in hepatoblastoma cells
Am J Physiol Gastrointest Liver Physiol,
January 1, 2006;
290(1):
G120 - G128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Kurzrock
Thrombopoietic Factors in Chronic Bone Marrow Failure States: The Platelet Problem Revisited
Clin. Cancer Res.,
February 15, 2005;
11(4):
1361 - 1367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Kuter and C. G. Begley
Recombinant human thrombopoietin: basic biology and evaluation of clinical studies
Blood,
November 15, 2002;
100(10):
3457 - 3469.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. C. Haznedaroglu, H. Goker, M. Turgut, Y. Buyukasik, and M. Benekli
Thrombopoietin as a Drug: Biologic Expectations, Clinical Realities, and Future Directions
Clinical and Applied Thrombosis/Hemostasis,
July 1, 2002;
8(3):
193 - 212.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E.-M. Wolber and W. Jelkmann
Thrombopoietin: The Novel Hepatic Hormone
Physiology,
February 1, 2002;
17(1):
6 - 10.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kaser, G. Brandacher, W. Steurer, S. Kaser, F. A. Offner, H. Zoller, I. Theurl, W. Widder, C. Molnar, O. Ludwiczek, et al.
Interleukin-6 stimulates thrombopoiesis through thrombopoietin: role in inflammatory thrombocytosis
Blood,
November 1, 2001;
98(9):
2720 - 2725.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ando, Y. Iwamoto, A. Suda, K. Tsuchiya, and H. Nihei
New insights into the thrombopoietic status of patients on dialysis through the evaluation of megakaryocytopoiesis in bone marrow and of endogenous thrombopoietin levels
Blood,
February 15, 2001;
97(4):
915 - 921.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Pertusini, J. Ratajczak, M. Majka, D. Vaughn, M. Z. Ratajczak, and A. M. Gewirtz
Investigating the platelet-sparing mechanism of paclitaxel/carboplatin combination chemotherapy
Blood,
February 1, 2001;
97(3):
638 - 644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Solanilla, J. Dechanet, A. El Andaloussi, M. Dupouy, F. Godard, J. Chabrol, P. Charbord, J. Reiffers, A. T. Nurden, B. Weksler, et al.
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells
Blood,
June 15, 2000;
95(12):
3758 - 3764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sungaran, O. T. Chisholm, B. Markovic, L. M. Khachigian, Y. Tanaka, and B. H. Chong
The role of platelet alpha -granular proteins in the regulation of thrombopoietin messenger RNA expression in human bone marrow stromal cells
Blood,
May 15, 2000;
95(10):
3094 - 3101.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hodohara, N. Fujii, N. Yamamoto, and K. Kaushansky
Stromal cell-derived factor-1 (SDF-1) acts together with thrombopoietin to enhance the development of megakaryocytic progenitor cells (CFU-MK)
Blood,
February 1, 2000;
95(3):
769 - 775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Peck-Radosavljevic, M. Wichlas, J. Zacherl, G. Stiegler, P. Stohlawetz, M. Fuchsjager, A. Kreil, S. Metz-Schimmerl, S. Panzer, R. Steininger, et al.
Thrombopoietin induces rapid resolution of thrombocytopenia after orthotopic liver transplantation through increased platelet production
Blood,
February 1, 2000;
95(3):
795 - 801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Yagi, K. A. Ritchie, E. Sitnicka, C. Storey, G. J. Roth, and S. Bartelmez
Sustained ex vivo expansion of hematopoietic stem cells mediated by thrombopoietin
PNAS,
July 6, 1999;
96(14):
8126 - 8131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E.-M. Wolber, C. Dame, H. Fahnenstich, D. Hofmann, P. Bartmann, W. Jelkmann, and J. Fandrey
Expression of the Thrombopoietin Gene in Human Fetal and Neonatal Tissues
Blood,
July 1, 1999;
94(1):
97 - 105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Cohen-Solal, N. Vitrat, M. Titeux, W. Vainchenker, and F. Wendling
High-Level Expression of Mpl in Platelets and Megakaryocytes Is Independent of Thrombopoietin
Blood,
May 1, 1999;
93(9):
2859 - 2866.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Chang, J. X. Qian, S. m. Lee, J. Joubran, G. Fernandez, J. Nichols, A. Knoppel, and J. S. Buzby
Tissue Uptake of Circulating Thrombopoietin Is Increased in Immune-Mediated Compared With Irradiated Thrombocytopenic Mice
Blood,
April 15, 1999;
93(8):
2515 - 2524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Ghilardi, A. Wiestner, and R. C. Skoda
Thrombopoietin Production Is Inhibited by a Translational Mechanism
Blood,
December 1, 1998;
92(11):
4023 - 4030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hirayama, S. Sakamaki, T. Matsunaga, T. Kuga, H. Kuroda, T. Kusakabe, K. Sasaki, K. Fujikawa, J. Kato, K. Kogawa, et al.
Concentrations of Thrombopoietin in Bone Marrow in Normal Subjects and in Patients With Idiopathic Thrombocytopenic Purpura, Aplastic Anemia, and Essential Thrombocythemia Correlate With Its mRNA Expression of Bone Marrow Stromal Cells
Blood,
July 1, 1998;
92(1):
46 - 52.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Cardier and J. Dempsey
Thrombopoietin and Its Receptor, c-mpl, Are Constitutively Expressed by Mouse Liver Endothelial Cells: Evidence of Thrombopoietin as a Growth Factor for Liver Endothelial Cells
Blood,
February 1, 1998;
91(3):
923 - 929.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Guerriero, L. Worford, H. K. Holland, G.-R. Guo, K. Sheehan, and E. K. Waller
Thrombopoietin Is Synthesized by Bone Marrow Stromal Cells
Blood,
November 1, 1997;
90(9):
3444 - 3455.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Komura-Naito, T. Matsumura, T. Sawada, T. Kato, and T. Tahara
Thrombopoietin in Patients With Hepatoblastoma
Blood,
October 1, 1997;
90(7):
2849 - 2849.
[Full Text]
[PDF]
|
|
|
|
|
