|
|
 Previous Article | Table of Contents | Next Article 
Telomerase activity in reactive and neoplastic lymphoid tissues: infrequent
detection of activity in Hodgkin's disease
P Brousset, T al Saati, N Chaouche, RC Zenou, D Schlaifer, S Chittal and G Delsol
Laboratoire du Groupe d'Etude des Lymphomes Malins (CNRS, CIGH), Toulouse,
France.
We used the recently described sensitive and rapid detection assay called
telomeric repeat amplification protocol (TRAP) to detect telomerase
activity in lymphoblastoid (n = 5) and lymphoma cell lines (n = 7),
hyperplastic lymph nodes (n = 6) and tonsils (n = 5), and tissues involved
by non-Hodgkin's lymphoma (NHL) (n = 43) and Hodgkin's disease (HD) (n =
14). Clearly evident telomerase activity was found in all lymphoblastoid
and lymphoma cell lines, and in 34 of 43 cases (80%) of NHL. These results
were expected because of the proliferative and immortal nature of the cell
lines and most malignant cells. However, positive results were obtained
with the TRAP assay in all hyperplastic lymph nodes and tonsils, which
raises the issue of derepression of telomerase activity during an immune
response. Telomerase activity alone therefore does not distinguish
malignant lymphoid proliferations from reactive states. Telomerase activity
was detected in only 1 of 14 cases (7%) of lymphoid tissues involved by HD.
Eight of the 13 negative cases were considered to be interpretable because
of the lack (3 of 13 cases) or low level (5 of 13 cases) of telomerase
inhibition. The five remaining cases could not be evaluated because of
their telomerase inhibitor content. The findings imply either transient or
very low levels of telomerase activity in HD or that HD for the greater
part is a telomerase-independent neoplasm. Microdissection studies are
needed to identify subsets of cells carrying telomerase activity in both
reactive and neoplastic lymphoid tissues.
Volume 89,
Issue 1,
pp. 26-31,
01/01/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. Yan, J.-M. Coindre, J. Benhattar, F. T. Bosman, and L. Guillou
Telomerase Activity and Human Telomerase Reverse Transcriptase mRNA Expression in Soft Tissue Tumors: Correlation with Grade, Histology, and Proliferative Activity
Cancer Res.,
July 1, 1999;
59(13):
3166 - 3170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-F. Norrback, G. Enblad, M. Erlanson, C. Sundstrom, and G. Roos
Telomerase Activity in Hodgkin's Disease
Blood,
July 15, 1998;
92(2):
567 - 573.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. W. Frenck Jr., E. H. Blackburn, and K. M. Shannon
The rate of telomere sequence loss in human leukocytes varies with age
PNAS,
May 12, 1998;
95(10):
5607 - 5610.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Brousset, N. Chaouche, F. Leprat, F. Branet-Brousset, H. Trouette, R. C. Zenou, J.-P. Merlio, and G. Delsol
Telomerase Activity in Human Thyroid Carcinomas Originating from the Follicular Cells
J. Clin. Endocrinol. Metab.,
December 1, 1997;
82(12):
4214 - 4216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Engelhardt, R. Kumar, J. Albanell, R. Pettengell, W. Han, and M. A.S. Moore
Telomerase Regulation, Cell Cycle, and Telomere Stability in Primitive Hematopoietic Cells
Blood,
July 1, 1997;
90(1):
182 - 193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
