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Enhancement of macrophage colony-stimulating factor-induced growth and
differentiation of human monocytes by interleukin-10
S Hashimoto, M Yamada, K Motoyoshi and KS Akagawa
Biochemical Research Laboratory, Morinaga Milk Industry Co, LTD,
Kanagawa-Pref., Japan.
Interleukin-10 (IL-10) has been reported to be a negative cytokine for
monocytes/macrophages. In the present study, we showed that IL-10 is rather
a positive cytokine and augments the growth and differentiation of human
monocytes stimulated with macrophage colony-stimulating factor (M-CSF).
Highly purified adherent human monocytes were cultured for 7 days with
M-CSF in the presence or absence of IL-10. The number of recovered cells
increased in the culture of monocytes with M-CSF + IL- 10 compared to the
culture with M-CSF alone. IL-10 alone was not enough to maintain the
survival and differentiation of monocytes into macrophages. Morphological
change cultured in M-CSF was also accelerated by addition of IL-10, and
macrophages cultured in M-CSF + IL-10 were more elongated compared to
macrophages cultured with M-CSF alone. Binding of 125I-M-CSF to monocytes
incubated with M-CSF + IL-10 was about 1.7-fold higher than that to
monocytes incubated with M-CSF alone. In accordance with the binding study,
Northern blot analysis showed that the levels of the expression of c-fms,
M-CSF receptor, mRNA in macrophages cultured in M-CSF + IL-10 were higher
than that in macrophages cultured in M-CSF alone. Macrophages cultured in
M-CSF + IL- 10 expressed higher level of Fc gamma RI, II, III, and showed
augmented Fc gamma receptor mediated phagocytosis. The former also produced
higher level of H2O2 and O2-, when stimulated with zymosan, and of IL-6
when stimulated with lipopolysaccharide compared to the latter. These
results taken together suggest that IL-10 augments the growth and
differentiation of human monocytes cultured in M-CSF.
Volume 89,
Issue 1,
pp. 315-321,
01/01/1997
Copyright © 1997 by The American Society of Hematology
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