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Lack of Fc-epsilon receptors on murine eosinophils: implications for the
functional significance of elevated IgE and eosinophils in parasitic
infections [see comments]
B de Andres, E Rakasz, M Hagen, ML McCormik, AL Mueller, D Elliot, A Metwali, M Sandor, BE Britigan, JV Weinstock and RG Lynch
Department of Pathology, The University of Iowa College of Medicine, Iowa
City 52242, USA.
Chronic infection with Schistosoma mansoni induces in humans and mice a
Th2-dominant immune response in which eosinophils and IgE are conspicuously
elevated. Human eosinophils express IgE receptors that participate in an
IgE-dependent eosinophil-mediated ADCC reaction against Schistosomula
larvae in vitro. To investigate the expression of IgE receptors on murine
eosinophils, they were purified (>95% pure by Giemsa-stained cytospin
preparations) from liver granulomas of Schistosoma-infected mice. Flow
cytometric analysis showed the absence of the low-affinity IgE receptor
Fc-epsilon RII (CD23) and Mac-2 and the absence of binding of murine IgE.
Reverse transcription-polymerase chain reaction (RT-PCR) analysis of
granuloma eosinophil mRNA did not detect transcripts for Fc-epsilon RII or
the alpha-chain of the high- affinity IgE receptor Fc-epsilon RI, but did
detect transcripts that encode Mac-2 and the low-affinity IgG receptors
Fc-gamma RIIb2, Fc- gamma RIII, and the FcR-associated gamma-chain. In
vitro stimulation of granuloma eosinophils with interleukin-4 (IL-4) did
not induce IgE binding, surface expression of Mac-2, or the transcription
of Fc- epsilon receptors (Fc-epsilon RI, Fc-epsilon RII/CD23). To
investigate normal murine eosinophils, we cultured normal mouse bone marrow
cells with recombinant IL-3, recombinant IL-5, and recombinant granulocyte-
macrophage colony-stimulating factor, conditions that promote eosinophil
differentiation. Flow cytometric analysis of bone marrow- derived
eosinophils failed to detect IgE binding or cell surface expression of
Fc-epsilon RII and Mac-2, and RT-PCR analysis of fluorescence-activated
cell sorted bone marrow-derived eosinophils failed to detect transcripts
that encode Fc-epsilon RI or Fc-epsilon RII. These findings show that, in
contrast to human eosinophils, murine eosinophils do not express cell
surface receptors that bind IgE. However, because IgG receptors (Fc-gamma
RIIb2, Fc-gamma RII) were present on eosinophils purified from granulomas,
we investigated whether they might be involved in eosinophil activation. We
found that an oxidative burst in eosinophils could be triggered through
their IgG receptors.
Volume 89,
Issue 10,
pp. 3826-3836,
05/15/1997
Copyright © 1997 by The American Society of Hematology
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