|
|
 Previous Article | Table of Contents | Next Article 
The conversion of fibrinogen to fibrin: recombinant fibrinogen typifies
plasma fibrinogen
OV Gorkun, YI Veklich, JW Weisel and ST Lord
Department of Pathology and Laboratory Medicine, University of North
Carolina, Chapel Hill 27599-7525, USA.
Plasma fibrinogen is a mixture of multiple molecular forms arising mainly
through alternative mRNA processing and subsequent posttranslational
modification. Recombinant fibrinogen is synthesized without alternative
mRNA processing in a cultured cell system that may generate novel
posttranslational modifications. Thus, to show that recombinant fibrinogen
can serve as a functional model for plasma fibrinogen, we have examined the
conversion of fibrinogen to fibrin, comparing the recombinant with the
plasma protein. We examined the kinetics of (1) thrombin-catalyzed
fibrinopeptide release, (2) thrombin- catalyzed polymerization of
fibrinogen, (3) the polymerization of fibrin monomers, and (4)
FXIIIa-catalyzed cross-link formation. We saw small differences in
polymerization, suggesting that the ordered assembly of protofibrils and
fibers was not identical. In all other analyses, we found that plasma
fibrinogen and recombinant fibrinogen were remarkably similar. Using
electron microscopy, we examined the structures of individual fibrinogen
molecules and fibrin clots. Individual fibrinogen molecules were
predominantly three nodule structures for both recombinant and plasma
proteins. Both samples also displayed four nodule structures, but fewer
four nodule structures were found with recombinant fibrinogen. Fibrin clot
structures were essentially indistinguishable. We concluded that
recombinant fibrinogen can serve as a accurate model for plasma fibrinogen.
Volume 89,
Issue 12,
pp. 4407-4414,
06/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Vu, C. Di Sanza, and M. Neerman-Arbez
Manipulating the quality control pathway in transfected cells: low temperature allows rescue of secretion-defective fibrinogen mutants
Haematologica,
February 1, 2008;
93(2):
224 - 231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Ajjan, B. C. B. Lim, K. F. Standeven, R. Harrand, S. Dolling, F. Phoenix, R. Greaves, R. H. Abou-Saleh, S. Connell, D. A. M. Smith, et al.
Common variation in the C-terminal region of the fibrinogen -chain: effects on fibrin structure, fibrinolysis and clot rigidity
Blood,
January 15, 2008;
111(2):
643 - 650.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Deng, J. P. Luyendyk, W. Zou, J. Lu, E. Malle, P. E. Ganey, and R. A. Roth
Neutrophil Interaction with the Hemostatic System Contributes to Liver Injury in Rats Cotreated with Lipopolysaccharide and Ranitidine
J. Pharmacol. Exp. Ther.,
August 1, 2007;
322(2):
852 - 861.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. I. Litvinov, O. V. Gorkun, D. K. Galanakis, S. Yakovlev, L. Medved, H. Shuman, and J. W. Weisel
Polymerization of fibrin: direct observation and quantification of individual B:b knob-hole interactions
Blood,
January 1, 2007;
109(1):
130 - 138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. I. Litvinov, O. V. Gorkun, S. F. Owen, H. Shuman, and J. W. Weisel
Polymerization of fibrin: specificity, strength, and stability of knob-hole interactions studied at the single-molecule level
Blood,
November 1, 2005;
106(9):
2944 - 2951.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Okumura, O. V. Gorkun, F. Terasawa, and S. T. Lord
Substitution of the {gamma}-chain Asn308 disturbs the D:D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linking
Blood,
June 1, 2004;
103(11):
4157 - 4163.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Moen, O. V. Gorkun, J. W. Weisel, and S. T. Lord
Recombinant B{beta}Arg14His fibrinogen implies participation of N-terminus of B{beta} chain in desA fibrin polymerization
Blood,
October 1, 2003;
102(7):
2466 - 2471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. B. Kuhns, E. L. Nelson, W. G. Alvord, and J. I. Gallin
Fibrinogen Induces IL-8 Synthesis in Human Neutrophils Stimulated with Formyl-Methionyl-Leucyl-Phenylalanine or Leukotriene B4
J. Immunol.,
September 1, 2001;
167(5):
2869 - 2878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ikari, K. Fujikawa, K. O. Yee, and S. M. Schwartz
alpha 1-Proteinase Inhibitor, alpha 1-Antichymotrypsin, or alpha 2-Macroglobulin Is Required for Vascular Smooth Muscle Cell Spreading in Three-dimensional Fibrin Gel
J. Biol. Chem.,
April 21, 2000;
275(17):
12799 - 12805.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Rooney, D. H. Farrell, B. M. van Hemel, P. G. de Groot, and S. T. Lord
The Contribution of the Three Hypothesized Integrin-Binding Sites in Fibrinogen to Platelet-Mediated Clot Retraction
Blood,
October 1, 1998;
92(7):
2374 - 2381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. O Yee, M. M. Rooney, C. M. Giachelli, S. T. Lord, and S. M. Schwartz
Role of ß1 and ß3 Integrins in Human Smooth Muscle Cell Adhesion to and Contraction of Fibrin Clots In Vitro
Circ. Res.,
August 10, 1998;
83(3):
241 - 251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Veklich, E. K. Ang, L. Lorand, and J. W. Weisel
The complementary aggregation sites of fibrin investigated through examination of polymers of fibrinogen with fragment E
PNAS,
February 17, 1998;
95(4):
1438 - 1442.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Okumura, O. V. Gorkun, and S. T. Lord
Severely Impaired Polymerization of Recombinant Fibrinogen gamma -364 Asp right-arrow His, the Substitution Discovered in a Heterozygous Individual
J. Biol. Chem.,
November 21, 1997;
272(47):
29596 - 29601.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Hogan, O. V. Gorkun, K. C. Lounes, A. I. Coates, J. W. Weisel, R. R. Hantgan, and S. T. Lord
Recombinant Fibrinogen Vlissingen/Frankfurt IV. THE DELETION OF RESIDUES 319 AND 320 FROM THE gamma CHAIN OF FIBRINOGEN ALTERS CALCIUM BINDING, FIBRIN POLYMERIZATION, CROSS-LINKING, AND PLATELET AGGREGATION
J. Biol. Chem.,
June 2, 2000;
275(23):
17778 - 17785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Hiller, A. Lichte, A. Oberpichler, A. Kocourek, and H. Tschesche
Matrix Metalloproteinases Collagenase-2, Macrophage Elastase, Collagenase-3, and Membrane Type 1-Matrix Metalloproteinase Impair Clotting by Degradation of Fibrinogen and Factor XII
J. Biol. Chem.,
October 13, 2000;
275(42):
33008 - 33013.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Mullin, O. V. Gorkun, C. G. Binnie, and S. T. Lord
Recombinant Fibrinogen Studies Reveal That Thrombin Specificity Dictates Order of Fibrinopeptide Release
J. Biol. Chem.,
August 11, 2000;
275(33):
25239 - 25246.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
