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Isolation and characterization of transformed human T-cell lines infected
by Epstein-Barr virus
H Groux, F Cottrez, C Montpellier, B Quatannens, J Coll, D Stehelin and C Auriault
CNRS-URA 1854 Institut Pasteur de Lille, France.
Epstein-Barr virus (EBV) is a human lymphotropic virus whose main targets
have traditionally been described as B lymphocytes and epithelial cells.
Here we report the isolation and characterization of largely monoclonal
transformed human T-cell lines infected by EBV. The transformed T cells
expressed CD2, CD3, and either CD4 or CD8 surface molecules and more
generally displayed the phenotype of naive T cells with a complete and
clonal rearrangement of the T-cell receptor. None of the cell lines
expressed B cells, natural killer, or myeloid antigens or had
immunoglobulins genes rearrangement. They grew in the absence of growth
factor; however, they all secreted interleukin-2 after mitogenic
activation. Polymerase chain reaction (PCR) analysis showed the presence of
EBV DNA in all these cell lines. Moreover, Southern blot analysis of one of
these cell lines shows the presence of circular episomic EBV DNA, and by
Northern blot or reverse transcriptase-PCR analysis, only the expression of
Epstein-Barr nuclear antigen-1 (EBNA-1) and latent membrane protein-1
(LMP-1) genes was detected. Finally, the complete transformed phenotype of
this T-cell line was shown by its injection into nude or recombination
activating gene 2 (RAG2)-deficient mice that led to the formation of solid
tumors.
Volume 89,
Issue 12,
pp. 4521-4530,
06/15/1997
Copyright © 1997 by The American Society of Hematology
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