|
|
 Previous Article | Table of Contents | Next Article 
Interactions between c-kit and stem cell factor are not required for B-
cell development in vivo
S Takeda, T Shimizu and HR Rodewald
Basel Institute for Immunology, Switzerland.
The receptor-type tyrosine kinase, c-kit is expressed in hematopoietic stem
cells (HSC), myeloid, and lymphoid precursors. In c-kit ligand- deficient
mice, absolute numbers of HSC are mildly reduced suggesting that c-kit is
not essential for HSC development. However, c-kit- HSC cannot form spleen
colonies or reconstitute hematopoietic functions in lethally irradiated
recipient mice. Based on in in vitro experiments, a critical role of c-kit
in B-cell development was suggested. Here we have investigated the B-cell
development of c-kit-null mutant (W/W) mice in vivo. Furthermore, day 13
fetal liver cells from wild type or W/W mice were transferred into
immunodeficient RAG-2-/- mice. Surprisingly, transferred c-kit- cells gave
rise to all stages of immature B cells in the bone marrow and subsequently
to mature conventional B2, as well as B1, type B cells in the recipients to
the same extent as transferred wild type cells. Hence, in contrast to
important roles of c-kit in the expansion of HSC and the generation of
erythroid and myeloid lineages and T-cell precursors, c-kit- HSC can
colonize the recipient bone marrow and differentiate into B cells in the
absence of c-kit.
Volume 89,
Issue 2,
pp. 518-525,
01/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
V. Agosti, S. Corbacioglu, I. Ehlers, C. Waskow, G. Sommer, G. Berrozpe, H. Kissel, C. M. Tucker, K. Manova, M. A.S. Moore, et al.
Critical Role for Kit-mediated Src Kinase But Not PI 3-Kinase Signaling in Pro T and Pro B Cell Development
J. Exp. Med.,
March 15, 2004;
199(6):
867 - 878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takaki, Y. Tezuka, K. Sauer, C. Kubo, S.-M. Kwon, E. Armstead, K. Nakao, M. Katsuki, R. M. Perlmutter, and K. Takatsu
Impaired Lymphopoiesis and Altered B Cell Subpopulations in Mice Overexpressing Lnk Adaptor Protein
J. Immunol.,
January 15, 2003;
170(2):
703 - 710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kouro, K. L. Medina, K. Oritani, and P. W. Kincade
Characteristics of early murine B-lymphocyte precursors and their direct sensitivity to negative regulators
Blood,
May 1, 2001;
97(9):
2708 - 2715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Colucci, D. Guy-Grand, A. Wilson, M. Turner, E. Schweighoffer, V. L. J. Tybulewicz, and J. P. Di Santo
A New Look at Syk in {alpha}{beta} and {gamma}{delta} T Cell Development Using Chimeric Mice with a Low Competitive Hematopoietic Environment
J. Immunol.,
May 15, 2000;
164(10):
5140 - 5145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ogawa, E. t. Boekel, and F. Melchers
Identification of CD19-B220+c-Kit+Flt3/Flk-2+cells as early B lymphoid precursors before pre-B-I cells in juvenile mouse bone marrow
Int. Immunol.,
March 1, 2000;
12(3):
313 - 324.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Colucci and J. P. Di Santo
The receptor tyrosine kinase c-kit provides a critical signal for survival, expansion, and maturation of mouse natural killer cells
Blood,
February 1, 2000;
95(3):
984 - 991.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. J. Payne, K. L. Medina, and P. W. Kincade
Loss of c-kit Accompanies B-Lineage Commitment and Acquisition of CD45R by Most Murine B-Lymphocyte Precursors
Blood,
July 15, 1999;
94(2):
713 - 723.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kawabata, M. Ujikawa, T. Egawa, H. Kawamoto, K. Tachibana, H. Iizasa, Y. Katsura, T. Kishimoto, and T. Nagasawa
A cell-autonomous requirement for CXCR4 in long-term lymphoid and myeloid reconstitution
PNAS,
May 11, 1999;
96(10):
5663 - 5667.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Colucci, C. Soudais, E. Rosmaraki, L. Vanes, V. L. J. Tybulewicz, and J. P. Di Santo
Dissecting NK Cell Development Using a Novel Alymphoid Mouse Model: Investigating the Role of the c-abl Proto-Oncogene in Murine NK Cell Differentiation
J. Immunol.,
March 1, 1999;
162(5):
2761 - 2765.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lampisuo, J. Liippo, O. Vainio, K. M. McNagny, J. Kulmala, and O. Lassila
Characterization of prethymic progenitors within the chicken embryo
Int. Immunol.,
January 1, 1999;
11(1):
63 - 69.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. D. Lyman and S. E. W. Jacobsen
c-kit Ligand and Flt3 Ligand: Stem/Progenitor Cell Factors With Overlapping Yet Distinct Activities
Blood,
February 15, 1998;
91(4):
1101 - 1134.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy
Stem Cell Factor and Hematopoiesis
Blood,
August 15, 1997;
90(4):
1345 - 1364.
[Full Text]
[PDF]
|
|
|
|
|
