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Characterization of adult alpha- and beta-globin genes in the zebrafish
FY Chan, J Robinson, A Brownlie, RA Shivdasani, A Donovan, C Brugnara, J Kim, BC Lau, HE Witkowska and LI Zon
Department of Biochemistry, Children's Hospital, Boston, MA 02115, USA.
Developmental switching of hemoglobins (Hbs) occurs in most vertebrates,
yet the cellular and molecular basis for this process remains elusive. The
zebrafish is a new genetic and developmental system that can be used to
study embryogenesis, and mutants with a variety of defects in hematopoiesis
have recently been derived. To initiate our studies on Hb switching in this
organism, we have characterized the globins expressed in the adult.
Reversed-phase high performance liquid chromatography and mass
spectrometric analyses of adult peripheral blood hemolysates showed that
there are three major alpha globins and two beta globins in circulating
erythroid cells. In addition, we have isolated and characterized zebrafish
adult alpha- and beta-globin cDNA clones that encode some of these globins.
High levels of alpha- and beta-globin gene expression were detected in
adult erythroid cells, whereas embryonic erythroid cells expressed little,
if any, of these RNAs. We have also shown that the alpha- and beta-globin
genes are tightly linked on the same chromosome and are arrayed in a 3'- 5'
to 5'-3' configuration, respectively. The characterization of these genes
and regulatory elements in this globin locus will provide insight into the
process of globin gene transcription. With these reagents, future studies
of Hb switching in zebrafish mutants with defective hematopoiesis will be
possible.
Volume 89,
Issue 2,
pp. 688-700,
01/15/1997
Copyright © 1997 by The American Society of Hematology
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