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P-selectin glycoprotein ligand-1 is essential for adhesion to P- selectin
but not E-selectin in stably transfected hematopoietic cell lines
KR Snapp, AJ Wagers, R Craig, LM Stoolman and GS Kansas
Department of Microbiology-Immunology, Northwestern Medical School,
Chicago, IL 60611, USA.
P-selectin (CD62P) is a member of the selectin family of adhesion molecules
involved in the regulation of leukocyte traffic. P-selectin glycoprotein
ligand-1 (PSGL-1) is a mucin-like molecule that is thought to be a primary
ligand for P-selectin. The interaction of P-selectin with PSGL-1 results in
leukocyte rolling and recruitment of leukocytes to sites of inflammation
and tissue injury. However, expression of PSGL- 1 protein alone is
insufficient for binding to P-selectin. Several posttranslational
modifications of PSGL-1, including sialylation, sulfation, and fucosylation
by alpha 1,3-fucosyltransferase(s) (FucT), are required for functional
interaction with P-selectin. Recently, several groups have reported that
PSGL-1 might also serve as a ligand for E-selectin. Differential
posttranslational modifications of PSGL-1 may determine whether it can
interact with either P- or E-selectin or both. To determine whether PSGL-1
is essential for adhesion to P- or E- selectin, we have constructed and
analyzed a panel of stably transfected K562 cells. K562 cells express
FucT-IV but not FucT-VII or PSGL-1, and do not bind to either E- or
P-selectin. K562 cells transfected with PSGL-1 cDNA also did not bind to
either P- or E- selectin. Binding to P-selectin occurred only when K562
cells were cotransfected with both FucT-VII and PSGL-1. In contrast,
expression of FucT-VII alone was sufficient for E-selectin binding. These
data demonstrate that expression of PSGL-1 is not required for adhesion of
a stably transfected hematopoietic cell line to E-selectin, and suggest
that FucT-IV alone cannot properly modify PSGL-1, expressed in transfected
K562 cells, to bind P-selectin.
Volume 89,
Issue 3,
pp. 896-901,
02/01/1997
Copyright © 1997 by The American Society of Hematology
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