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Thrombopoietin enhances proliferation and differentiation of murine yolk
sac erythroid progenitors
T Era, T Takahashi, K Sakai, K Kawamura and T Nakano
Department of Molecular Cell Biology, Research Institute for Microbial
Diseases, Osaka University, Suita, Japan.
Thrombopoietin (TPO), the ligand for the receptor proto-oncogene c-Mpl, has
been cloned and shown to be the critical regulator of proliferation and
differentiation of megakaryocytic lineage. Initially, TPO was not
considered to have the activity on hematopoietic lineages other than
megakaryocytes. Recently, however, TPO was reported to enhance the in vitro
erythroid colony formation from human bone marrow (BM) CD34+ progenitors or
from mouse BM cells in combination with other cytokines. We examined the
effects of TPO on the colony formation of hematopoietic progenitors in
mouse yolk sac. TPO remarkably enhanced proliferation and differentiation
of erythroid-lineage cells in the presence of erythropoietin (Epo). This
effect was observed even in the absence of Epo. Compared with adult BM,
yolk sac turned out to have relatively abundant erythroid and
erythro-megakaryocytic progenitors, which responded to TPO and Epo
stimulation. TPO similarly stimulated erythroid colony formation from in
vitro differentiation-induced mouse embryonic stem (ES) cells whose
hematopoietic differentiation status was similar to that of yolk sac. These
findings help to understand the biology of hematopoietic progenitors of the
early phase of hematopoiesis. Yolk sac cells or in vitro
differentiation-induced ES cells would be good sources to analyze the TPO
function on erythropoiesis.
Volume 89,
Issue 4,
pp. 1207-1213,
02/15/1997
Copyright © 1997 by The American Society of Hematology
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