Host F1 mice pretreated with granulocyte colony-stimulating factor accept
parental bone marrow grafts in hybrid resistance system
K Iizuka, H Kaneko, T Yamada, H Kimura, Y Kokai and J Fujimoto
Department of Pathology, National Children's Medical Research Center,
Tokyo, Japan.
In the setting of hybrid resistance, parental C57BL/6 bone marrow (BM)
grafts are vigorously rejected by lethally irradiated (C57BL/6xDBA/2) F1
mice. However, F1 mice pretreated by continuous administration of
granulocyte colony-stimulating factor (G-CSF) with a miniosmotic pump
before BM grafting developed day-8 splenic colonies of donor origin. This
inhibitory effect on rejection was reversible because F1 mice regained the
capacity to reject parental BM when the pump ceased functioning. The
appearance of only a small number of colonies with the administration of
G-CSF soon after BM grafting suggested the importance in producing this
inhibitory effect of pre-exposure of host mice to G- CSF. Because G-CSF
administration with a syngeneic combination did not influence the number of
colonies, an altered distribution of grafted precursors was unlikely. The
absence of a reduction in the number of NK1.1-positive cells in
G-CSF-treated mice suggested functional impairment of natural killer cells,
major effectors in hybrid resistance, but further study is necessary to
elucidate the mechanism underlying this phenomenon. However, our results
indicate the importance of G-CSF as a regulator in a certain type of immune
response and raise the possibility of clinical application in
transplantation medicine.
Volume 89,
Issue 4,
pp. 1446-1451,
02/15/1997
Copyright © 1997 by The American Society of Hematology
