|
|
 Previous Article | Table of Contents | Next Article 
Mouse strain-dependent changes in frequency and proliferation of
hematopoietic stem cells during aging: correlation between lifespan and
cycling activity
G de Haan, W Nijhof and G Van Zant
University of Kentucky Medical Center, Division of Hematology/Oncology,
Markey Cancer Center, Lexington 40536-0093, USA.
We have quantified the frequency and proliferation of five subsets of
primitive hematopoietic cells, using the cobblestone area forming cell
(CAFC) assay, in marrow of five strains of mice with lifespans ranging from
about 500 to 800 days. Stem cell characteristics were determined in young
(6 weeks) and old (12 months) mice. We report striking effects of both
intrinsic strain lifespan and individual mouse age on stem cell
populations. First, the relative and absolute numbers of the most primitive
stem cell subsets was threefold to fourfold higher in old than in young
mice. Second, a considerable strain-to-strain variation in the number of
primitive cells was observed: when absolute frequencies were calculated,
there was a trend for longer lifespan to be correlated with a larger stem
cell pool. Third, stem cells from old mice had a far lower cycling activity
than cells from young mice. However, this was highly strain dependent:
short-lived C3H/He and CBA/J mice showed a stronger reduction in cycling
activity during aging than long-lived C57BL/6 mice. Finally, a significant
negative correlation was demonstrated in young mice between maximal
lifespan and proliferative activity. These data show that aging has a major
impact on the frequency and cell-cycle kinetics of primitive hematopoietic
cell compartments. In addition, the observation that cycling activity of
stem cells is related to the maximal lifespan of the mouse strain may open
ways to identify the genetic mechanisms of both strain- and age-dependent
variation in the structure of primitive hematopoietic cell compartments.
Volume 89,
Issue 5,
pp. 1543-1550,
03/01/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. H. Cho, H. B. Sieburg, and C. E. Muller-Sieburg
A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells
Blood,
June 15, 2008;
111(12):
5553 - 5561.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Jawad, G Giotopoulos, C Cole, and M Plumb
Target cell frequency is a genetically determined risk factor in radiation leukaemogenesis
Br. J. Radiol.,
September 1, 2007;
80(Special_Issue_1):
S56 - S62.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Pearce, F. Anjos-Afonso, C. M. Ridler, A. Eddaoudi, and D. Bonnet
Age-Dependent Increase in Side Population Distribution Within Hematopoiesis: Implications for Our Understanding of the Mechanism of Aging
Stem Cells,
April 1, 2007;
25(4):
828 - 835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. van Os, L. M. Kamminga, A. Ausema, L. V. Bystrykh, D. P. Draijer, K. van Pelt, B. Dontje, and G. de Haan
A Limited Role for p21Cip1/Waf1 in Maintaining Normal Hematopoietic Stem Cell Functioning
Stem Cells,
April 1, 2007;
25(4):
836 - 843.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Dumble, L. Moore, S. M. Chambers, H. Geiger, G. Van Zant, M. A. Goodell, and L. A. Donehower
The impact of altered p53 dosage on hematopoietic stem cell dynamics during aging
Blood,
February 15, 2007;
109(4):
1736 - 1742.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Kamminga and G. de Haan
Cellular Memory and Hematopoietic Stem Cell Aging
Stem Cells,
May 1, 2006;
24(5):
1143 - 1149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. C. Schatteman and N. Ma
Old Bone Marrow Cells Inhibit Skin Wound Vascularization
Stem Cells,
March 1, 2006;
24(3):
717 - 721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Kamminga, L. V. Bystrykh, A. de Boer, S. Houwer, J. Douma, E. Weersing, B. Dontje, and G. de Haan
The Polycomb group gene Ezh2 prevents hematopoietic stem cell exhaustion
Blood,
March 1, 2006;
107(5):
2170 - 2179.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. H. Yilmaz, M. J. Kiel, and S. J. Morrison
SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity
Blood,
February 1, 2006;
107(3):
924 - 930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Rossi, D. Bryder, J. M. Zahn, H. Ahlenius, R. Sonu, A. J. Wagers, and I. L. Weissman
Cell intrinsic alterations underlie hematopoietic stem cell aging
PNAS,
June 28, 2005;
102(26):
9194 - 9199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Geiger, G. Rennebeck, and G. Van Zant
Regulation of hematopoietic stem cell aging in vivo by a distinct genetic element
PNAS,
April 5, 2005;
102(14):
5102 - 5107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Smith, F. M. Ellison, J. P. McCoy Jr., and J. Chen
c-Kit Expression and Stem Cell Factor-Induced Hematopoietic Cell Proliferation Are Up-Regulated in Aged B6D2F1 Mice
J. Gerontol. A Biol. Sci. Med. Sci.,
April 1, 2005;
60(4):
448 - 456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. M. Chilton, F. Rezzoug, M. Z. Ratajczak, I. Fugier-Vivier, J. Ratajczak, M. Kucia, Y. Huang, M. K. Tanner, and S. T. Ildstad
Hematopoietic stem cells from NOD mice exhibit autonomous behavior and a competitive advantage in allogeneic recipients
Blood,
March 1, 2005;
105(5):
2189 - 2197.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Roeder, L. M. Kamminga, K. Braesel, B. Dontje, G. de Haan, and M. Loeffler
Competitive clonal hematopoiesis in mouse chimeras explained by a stochastic model of stem cell organization
Blood,
January 15, 2005;
105(2):
609 - 616.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Kamminga, R. van Os, A. Ausema, E. J. K. Noach, E. Weersing, B. Dontje, E. Vellenga, and G. de Haan
Impaired Hematopoietic Stem Cell Functioning After Serial Transplantation and During Normal Aging
Stem Cells,
January 1, 2005;
23(1):
82 - 92.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Henckaerts, J. C. Langer, J. Orenstein, and H.-W. Snoeck
The Positive Regulatory Effect of TGF-{beta}2 on Primitive Murine Hemopoietic Stem and Progenitor Cells Is Dependent on Age, Genetic Background, and Serum Factors
J. Immunol.,
August 15, 2004;
173(4):
2486 - 2493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Henckaerts, J. C. Langer, and H.-W. Snoeck
Quantitative genetic variation in the hematopoietic stem cell and progenitor cell compartment and in lifespan are closely linked at multiple loci in BXD recombinant inbred mice
Blood,
July 15, 2004;
104(2):
374 - 379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Boulton, C. Cole, A. Knight, H. Cleary, R. Snowden, and M. Plumb
Low-penetrance genetic susceptibility and resistance loci implicated in the relative risk for radiation-induced acute myeloid leukemia in mice
Blood,
March 15, 2003;
101(6):
2349 - 2354.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. de Haan, L. V. Bystrykh, E. Weersing, B. Dontje, H. Geiger, N. Ivanova, I. R. Lemischka, E. Vellenga, and G. Van Zant
A genetic and genomic analysis identifies a cluster of genes associated with hematopoietic cell turnover
Blood,
August 28, 2002;
100(6):
2056 - 2062.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Geiger, J. M. True, B. Grimes, E. J. Carroll, R. A. Fleischman, and G. Van Zant
Analysis of the hematopoietic potential of muscle-derived cells in mice
Blood,
June 28, 2002;
100(2):
721 - 723.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Morrison, D. Qian, L. Jerabek, B. A. Thiel, I.-K. Park, P. S. Ford, M. J. Kiel, N. J. Schork, I. L. Weissman, and M. F. Clarke
A Genetic Determinant That Specifically Regulates the Frequency of Hematopoietic Stem Cells
J. Immunol.,
January 15, 2002;
168(2):
635 - 642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Chen and D. E. Harrison
Quantitative trait loci regulating relative lymphocyte proportions in mouse peripheral blood
Blood,
January 15, 2002;
99(2):
561 - 566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Geiger, J. M. True, G. de Haan, and G. Van Zant
Age- and stage-specific regulation patterns in the hematopoietic stem cell hierarchy
Blood,
November 15, 2001;
98(10):
2966 - 2972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Levin, L. Cocault, C. Demerens, C. Challier, M. Pauchard, J. Caen, and M. Souyri
Thrombocytopenic c-mpl{-}/{-} mice can produce a normal level of platelets after administration of 5-fluorouracil: the effect of age on the response
Blood,
August 15, 2001;
98(4):
1019 - 1027.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Zaucha, C. Yu, G. Mathioudakis, K. Seidel, G. Georges, G. Sale, M.-T. Little, B. Torok-Storb, and R. Storb
Hematopoietic responses to stress conditions in young dogs compared with elderly dogs
Blood,
July 15, 2001;
98(2):
322 - 327.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Chen, C. M. Astle, C. E. Muller-Sieburg, and D. E. Harrison
Primitive hematopoietic stem cell function in vivo is uniquely high in the CXB-12 mouse strain
Blood,
December 15, 2000;
96(13):
4124 - 4131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. de Haan, S. J. Szilvassy, T. E. Meyerrose, B. Dontje, B. Grimes, and G. Van Zant
Distinct functional properties of highly purified hematopoietic stem cells from mouse strains differing in stem cell numbers
Blood,
August 15, 2000;
96(4):
1374 - 1379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Okada, T. Yoshida, Z. Hong, G. Ishii, M. Hatano, M. Kuro-o, Y. Nabeshima, Y.-i. Nabeshima, and T. Tokuhisa
Impairment of B lymphopoiesis in precocious aging (klotho) mice
Int. Immunol.,
June 1, 2000;
12(6):
861 - 871.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Muller-Sieburg, R. H. Cho, H. B. Sieburg, S. Kupriyanov, and R. Riblet
Genetic control of hematopoietic stem cell frequency in mice is mostly cell autonomous
Blood,
April 1, 2000;
95(7):
2446 - 2448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. van Os, S. Robinson, T. Sheridan, and P. M. Mauch
Granulocyte-Colony Stimulating Factor Impedes Recovery from Damage Caused by Cytotoxic Agents through Increased Differentiation at the Expense of Self-Renewal
Stem Cells,
March 1, 2000;
18(2):
120 - 127.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Tremml, C. Dominguez, V. Rosti, Z. Zhang, P.P. Pandolfi, P. Keller, and M. Bessler
Increased Sensitivity to Complement and a Decreased Red Blood Cell Life Span in Mice Mosaic for a Nonfunctional Piga Gene
Blood,
November 1, 1999;
94(9):
2945 - 2954.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Keller, G. Tremml, V. Rosti, and M. Bessler
X inactivation and somatic cell selection rescue female mice carrying a Piga-null mutation
PNAS,
June 22, 1999;
96(13):
7479 - 7483.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. de Haan and G. Van Zant
Dynamic Changes in Mouse Hematopoietic Stem Cell Numbers During Aging
Blood,
May 15, 1999;
93(10):
3294 - 3301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. DE HAAN and G. VAN ZANT
Genetic analysis of hemopoietic cell cycling in mice suggests its involvement in organismal life span
FASEB J,
April 1, 1999;
13(6):
707 - 713.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. H. Cheshier, S. J. Morrison, X. Liao, and I. L. Weissman
In vivo proliferation and cell cycle kinetics of long-term self-renewing hematopoietic stem cells
PNAS,
March 16, 1999;
96(6):
3120 - 3125.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Uchida, A. M. Friera, D. He, M. J. Reitsma, A. S. Tsukamoto, and I. L. Weissman
Hydroxyurea Can Be Used to Increase Mouse c-kit+Thy-1.1loLin-/loSca-1+ Hematopoietic Cell Number and Frequency in Cell Cycle In Vivo
Blood,
December 1, 1997;
90(11):
4354 - 4362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. d. Haan and G. V. Zant
Intrinsic and Extrinsic Control of Hemopoietic Stem Cell Numbers: Mapping of a Stem Cell Gene
J. Exp. Med.,
August 18, 1997;
186(4):
529 - 536.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
