|
|
 Previous Article | Table of Contents | Next Article 
Constitutive activation of U937 promonocytic cell clones selected for their
resistance to parvovirus H-1 infection
JA Lopez-Guerrero, B Rayet, M Tuynder, J Rommelaere and C Dinsart
Deutsches Krebsforschungszentrum, Applied Tumor Virology, Heidelberg,
Germany.
The human promonocytic cell line U937 is highly sensitive to the lytic
effect of the autonomous parvovirus H-1. Rare cell variants that resisted
H-1 virus infection could be isolated, of which four (RU1, RU2, RU3, and
RU4) were further characterized. In contrast to parental cells, the RU
clones sustained an abortive H-1 virus infection. Three of the clones
showed a significant decrease in the accumulation levels of the c-Myc
oncoprotein and in their capacity for forming tumors in immunodeficient
mice. Surprisingly, all RU clones resisted the suppressing effect of
12-O-tetradecanoylphorbol-13-acetate (TPA) on c- myc oncogene expression
and cell proliferation. In contrast, RU clones exhibited the TPA-induced
changes in membrane surface antigens and nonspecific esterase activities
that are characteristic of monocytic differentiation. Studies of the
activation steady-state of RU cells demonstrated the constitutive
production of significant amounts of nitric oxide (NO) and superoxide anion
(O-.2). Inhibitors of NO and O- .2, production sensitized all RU cells to
the killing effect of parvovirus H-1 and increased the production of
infectious viral particles. These data argue for the participation of
active oxygen species in macrophage defence mechanisms against parvovirus
infection. Moreover, the use of parvovirus H-1 as a selective agent in a
cell- colony formation assay allowed us to show that expression of defined
markers of monocytic differentiation can be uncoupled from suppression of
proliferation.
Volume 89,
Issue 5,
pp. 1642-1653,
03/01/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
H. Iseki, R. Shimizukawa, F. Sugiyama, S. Kunita, A. Iwama, M. Onodera, H. Nakauchi, and K.-i. Yagami
Parvovirus Nonstructural Proteins Induce an Epigenetic Modification through Histone Acetylation in Host Genes and Revert Tumor Malignancy to Benignancy
J. Virol.,
July 15, 2005;
79(14):
8886 - 8893.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Li, E. Werner, M. Hergenhahn, R. Poirey, Z. Luo, J. Rommelaere, and J.-C. Jauniaux
Expression Profiling of Human Hepatoma Cells Reveals Global Repression of Genes Involved in Cell Proliferation, Growth, and Apoptosis upon Infection with Parvovirus H-1
J. Virol.,
February 15, 2005;
79(4):
2274 - 2286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ueno, T. Harada, H. Iseki, T. Ohshima, F. Sugiyama, and K.-i. Yagami
Propagation of Rat Parvovirus in Thymic Lymphoma Cell Line C58(NT)D and Subsequent Appearance of a Resistant Cell Clone after Lytic Infection
J. Virol.,
April 15, 2001;
75(8):
3965 - 3970.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Herbein and W. A. O'brien
Tumor Necrosis Factor (TNF)-{alpha} and TNF Receptors in Viral Pathogenesis
Experimental Biology and Medicine,
March 1, 2000;
223(3):
241 - 257.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. Rayet, J.-A. Lopez-Guerrero, J. Rommelaere, and C. Dinsart
Induction of Programmed Cell Death by Parvovirus H-1 in U937 Cells: Connection with the Tumor Necrosis Factor Alpha Signalling Pathway
J. Virol.,
November 1, 1998;
72(11):
8893 - 8903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Lopez-Guerrero and L. Carrasco
Effect of Nitric Oxide on Poliovirus Infection of Two Human Cell Lines
J. Virol.,
March 1, 1998;
72(3):
2538 - 2540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
