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Is Kaposi's sarcoma--associated herpesvirus ubiquitous in urogenital and
prostate tissues?
T Tasaka, JW Said, R Morosetti, D Park, W Verbeek, M Nagai, J Takahara and HP Koeffler
Department of Medicine and Pathology, Cedars-Sinai Medical Center, UCLA
School of Medicine, USA.
Controversy exists as to whether Kaposi's sarcoma-associated herpesvirus
(KSHV) is more widespread than originally reported. Recently, Monini et al
reported that KSHV is ubiquitous in urogenital and prostate tissues and
sperm of healthy Italian adults using nested polymerase chain reaction
(PCR). We have examined for the presence of KSHV in 10 normal prostates
from Italian men and 10 from men from the United States, as well as 32
prostatic, 30 vulvar, 24 ovarian, 20 cervical, and 30 testicular cancer
specimens from patients from the United States. None of the patients had a
history of human immunodeficiency virus infection. The samples were tested
by nested PCR. The sensitivity of this assay was determined by a dilution
study performed by diluting KSHV DNA from the KS-1 cells (a primary
effusion lymphoma cell line which is estimated to have 16 copies of KSHV
per cell) in DNA from a K562 myeloid cell line. The nested PCR that we used
can detect 2.4 copies of KSHV sequences on a background of K562 DNA. All
the samples were negative for KSHV sequences. Therefore, we cannot confirm
the finding that KSHV sequences are ubiquitous in urogenital and prostate
tissues. Furthermore, because our samples were from both the United States
and Italy, the discrepancy between results is unlikely to be explained by
either ethnic or environmental factors. False-positive results easily occur
using nested primer PCR because of contamination. Our data argue that KSHV
is not widely disseminated in urogenital tissues from nonimmunosuppressed
individuals.
Volume 89,
Issue 5,
pp. 1686-1689,
03/01/1997
Copyright © 1997 by The American Society of Hematology
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