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 Previous Article | Table of Contents | Next Article 
1,25-dihydroxyvitamin D3 induces NAD(+)-dependent 15- hydroxyprostaglandin
dehydrogenase in human neonatal monocytes
F Pichaud, S Roux, JL Frendo, R Delage-Mourroux, J Maclouf, MC de Vernejoul, MS Moukhtar and A Jullienne
U. 349 and U. 348 INSERM, Hopital Lariboisiere, centre Viggo Petersen,
Paris, France.
1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3] induces the differentiation of
monocytes into macrophage-like cells in vitro. To identify the genes
expressed during this process, we performed differential display polymerase
chain reaction on RNA extracted from cord blood monocytes (CBMs) treated
with 1,25-(OH)2D3. Treated CBMs expressed type-I 15- hydroxyprostaglandin
dehydrogenase (type-I 15-PGDH), the key enzyme of prostaglandin E2 (PGE2)
catabolism and a 15-PGDH-related mRNA (15- PGDHr). This newly described
15-PGDH-related mRNA was constitutively expressed in adult monocytes.
15-PGDH gene(s) transcription was accompanied by the appearance of the
15-PGDH activity in treated CBMs. In addition, the cyclooxygenase 2 mRNA
level was decreased and PGE2 levels in the culture mediums were lowered
(50%). Our results stress that 1,25-(OH)2D3, at least in neonatal
monocytes, can exert, directly or indirectly, a dual control on key enzymes
of PGE2 metabolism. In conclusion, we suggest that modifications in
prostaglandin metabolism, induced by the expression of type-I 15-PGDH and
the downregulation of cyclooxygenase 2, could be involved in monocytic
differentiation.
Volume 89,
Issue 6,
pp. 2105-2112,
03/15/1997
Copyright © 1997 by The American Society of Hematology
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