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HPA-10w(b) (La(a)): genetic determination of a new platelet-specific
alloantigen on glycoprotein IIIa and its expression in COS-7 cells
O Peyruchaud, F Bourre, MC Morel-Kopp, D Reviron, P Mercier, A Nurden and C Kaplan
Unite Mixte de Recherche (UMR) 5533 Centre National de la Recherche
Scientifique (CNRS), Hopital Cardiologique, Pessac, France.
The heterodimeric complex glycoprotein (GP)IIb-IIIa, the fibrinogen
receptor of platelets, carries numerous alloantigen systems. These
polymorphisms are responsible for the immune response after transfusion or
during pregnancy. In the latter case, the mother develops an antibody
against an epitope present on fetal platelets, and this results in platelet
destruction in the fetus. In this report, we describe the molecular
characterization of a new alloantigen (La(a)) on GPIIIa responsible for
neonatal alloimmune thrombocytopenia (NAIT). Using polymerase chain
reaction (PCR)-single-strand conformation polymorphism (SSCP) and DNA
sequencing, we found a point mutation (G to A) in a heterozygous state on
the GPIIIa gene leading to amino acid substitution Arg to Gln at position
62 of the mature protein. Transient expression of GPIIb-IIIa complexes in
Cos-7 cells using wild-type or mutated GPIIIa cDNA allowed us to
demonstrate that this mutation was responsible for expression of the La(a)
epitope.
Volume 89,
Issue 7,
pp. 2422-2428,
04/01/1997
Copyright © 1997 by The American Society of Hematology
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