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The characterization, molecular cloning, and expression of a novel
hematopoietic cell antigen from CD34+ human bone marrow cells
N Uchida, Z Yang, J Combs, O Pourquie, M Nguyen, R Ramanathan, J Fu, A Welply, S Chen, G Weddell, AK Sharma, KR Leiby, D Karagogeos, B Hill, L Humeau, WB Stallcup, R Hoffman, AS Tsukamoto, DP Gearing and B Peault
SyStemix Inc, Palo Alto, CA 94304, USA.
The adhesion molecule BEN/SC1/DM-GRASP (BEN) is a marker in the developing
chicken nervous system that is also expressed on the surface of embryonic
and adult hematopoietic cells such as immature thymocytes, myeloid
progenitors, and erythroid progenitors. F84.1 and KG-CAM, two monoclonal
antibodies to rat neuronal glycoproteins with similarity to BEN,
cross-react with an antigen on rat hematopoietic progenitors, but F84.1
only also recognizes human blood cell progenitors. We have defined the
antigen recognized by F84.1 as the hematopoietic cell antigen (HCA). HCA
expression was detected on 40% to 70% of CD34+ fetal and adult bone marrow
cells and mobilized peripheral blood cells. Precursor cell activity for
long-term in vitro bone marrow cell culture was confined to the subset of
CD34+ cells that coexpress HCA. HCA is expressed by the most primitive
subsets of CD34+ cells, including all rhodamine 123(lo), Thy-1+, and
CD38(-/lo) CD34+ adult bone marrow cells. HCA was also detected on myeloid
progenitors but not on early B- cell progenitors. We also describe here the
cloning and characterization of cDNAs encoding two variants of the human
HCA antigen (huHCA-1 and huHCA-2) and of a cDNA clone encoding rat HCA
(raHCA). The deduced amino acid sequences of huHCA and raHCA are homologous
to that of chicken BEN. Recombinant proteins produced from either human or
rat HCA cDNAs were recognized by F84.1, whereas rat HCA but not human HCA
was recognized by antirat KG-CAM. Expression of either form of huHCA in CHO
cells conferred homophilic adhesion that could be competed with soluble
recombinant huHCA-Fc. The molecular cloning of HCA and the availability of
recombinant HCA should permit further evaluation of its role in human and
rodent hematopoiesis.
Volume 89,
Issue 8,
pp. 2706-2716,
04/15/1997
Copyright © 1997 by The American Society of Hematology
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