|
|
 Previous Article | Table of Contents | Next Article 
Strict lymphotropism of Epstein-Barr virus during acute infectious
mononucleosis in nonimmunocompromised individuals
MA Karajannis, M Hummel, I Anagnostopoulos and H Stein
Institute of Pathology, Klinikum Benjamin Franklin, Free University Berlin,
Germany.
Previous investigations of exfoliated oropharyngeal cells from individuals
suffering from infectious mononucleosis (IM) suggested that the
oropharyngeal epithelia are the primary target and also the site of
life-long persistence of the Epstein-Barr virus (EBV). This concept was
widely accepted. However, the investigation of histological sections with
more sensitive EBV detection techniques has drawn this concept into doubt
since EBV proved to be constantly absent in normal epithelial cells. To
elucidate the discrepancy, throat washings and peripheral mononuclear blood
cells from 16 patients suffering from IM were investigated for EBV-DNA and
EBV gene products employing highly sensitive in situ hybridization,
immunocytochemistry, and polymerase chain reaction. Although all patients
exhibited latently infected B lymphocytes in peripheral blood, samples of
exfoliated oropharyngeal cells were constantly EBV-negative with the
exception of three cases. In these cases, the patients additionally
suffered from purulent ulcerating tonsillitis, EBV-infected B cells, but no
EBV-infected epithelial cells were detectable. These findings support the
view that recirculating lymphocytes of B-cell origin, but not epithelial
cells are the initial target of EBV during primary infection and that B
cells also represent the site of life-long viral persistence.
Volume 89,
Issue 8,
pp. 2856-2862,
04/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Shannon-Lowe, E. Adland, A. I. Bell, H.-J. Delecluse, A. B. Rickinson, and M. Rowe
Features Distinguishing Epstein-Barr Virus Infections of Epithelial Cells and B Cells: Viral Genome Expression, Genome Maintenance, and Genome Amplification
J. Virol.,
August 1, 2009;
83(15):
7749 - 7760.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. W. Bornkamm, U. Behrends, and J. Mautner
The infectious kiss: Newly infected B cells deliver Epstein-Barr virus to epithelial cells
PNAS,
May 9, 2006;
103(19):
7201 - 7202.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Pegtel, J. Middeldorp, and D. A. Thorley-Lawson
Epstein-Barr Virus Infection in Ex Vivo Tonsil Epithelial Cell Cultures of Asymptomatic Carriers
J. Virol.,
November 15, 2004;
78(22):
12613 - 12624.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Kutok, S. Klumpp, M. Simon, J. J. MacKey, V. Nguyen, J. M. Middeldorp, J. C. Aster, and F. Wang
Molecular Evidence for Rhesus Lymphocryptovirus Infection of Epithelial Cells in Immunosuppressed Rhesus Macaques
J. Virol.,
April 1, 2004;
78(7):
3455 - 3461.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Herrmann, P. Frangou, J. Middeldorp, and G. Niedobitek
Epstein-Barr virus replication in tongue epithelial cells
J. Gen. Virol.,
December 1, 2002;
83(12):
2995 - 2998.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P G Murray and L S Young
Themed issue: the biology and pathology of the Epstein-Barr virus
Mol. Pathol.,
October 1, 2000;
53(5):
219 - 221.
[Full Text]
|
|
|
|
|
|
|
G Niedobitek
Epstein-Barr virus infection in the pathogenesis of nasopharyngeal carcinoma
Mol. Pathol.,
October 1, 2000;
53(5):
248 - 254.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Spieker and H. Herbst
Distribution and Phenotype of Epstein-Barr Virus-Infected Cells in Inflammatory Bowel Disease
Am. J. Pathol.,
July 1, 2000;
157(1):
51 - 57.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G Niedobitek, A Agathanggelou, N Steven, and L S Young
Epstein-Barr virus (EBV) in infectious mononucleosis: detection of the virus in tonsillar B lymphocytes but not in desquamated oropharyngeal epithelial cells
Mol. Pathol.,
February 1, 2000;
53(1):
37 - 42.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Yoda, T. Sata, T. Kurata, and H. Aramaki
Oropharyngotonsillitis Associated With Nonprimary Epstein-Barr Virus Infection
Arch Otolaryngol Head Neck Surg,
February 1, 2000;
126(2):
185 - 193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Tanner and C. Alfieri
Epstein-Barr Virus Induces Fas (CD95) in T Cells and Fas Ligand in B Cells Leading to T-Cell Apoptosis
Blood,
November 15, 1999;
94(10):
3439 - 3447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. S. Misko, S. M. Cross, R. Khanna, S. L. Elliott, C. Schmidt, S. J. Pye, and S. L. Silins
Crossreactive recognition of viral, self, and bacterial peptide ligands by human class I-restricted cytotoxic T lymphocyte clonotypes: Implications for molecular mimicry in autoimmune disease
PNAS,
March 2, 1999;
96(5):
2279 - 2284.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Fingeroth, M. E. Diamond, D. R. Sage, J. Hayman, and J. L. Yates
CD21-Dependent Infection of an Epithelial Cell Line, 293, by Epstein-Barr Virus
J. Virol.,
March 1, 1999;
73(3):
2115 - 2125.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. C. Faulkner, S. R. Burrows, R. Khanna, D. J. Moss, A. G. Bird, and D. H. Crawford
X-Linked Agammaglobulinemia Patients Are Not Infected with Epstein-Barr Virus: Implications for the Biology of the Virus
J. Virol.,
February 1, 1999;
73(2):
1555 - 1564.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. D. Hislop, N. E. Annels, N. H. Gudgeon, A. M. Leese, and A. B. Rickinson
Epitope-specific Evolution of Human CD8+ T Cell Responses from Primary to Persistent Phases of Epstein-Barr Virus Infection
J. Exp. Med.,
April 1, 2002;
195(7):
893 - 905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
