|
|
 Previous Article | Table of Contents | Next Article 
The expression of Bcl-x is downregulated during differentiation of human
hematopoietic progenitor cells along the granulocyte but not the
monocyte/macrophage lineage
C Sanz, A Benito, M Silva, B Albella, C Richard, JC Segovia, A Insunza, JA Bueren and JL Fernandez-Luna
Servicio de Immunologia, Hospital Universitario Marques de Valdecilla,
Insalud, Santander, Spain.
Expression of the apoptosis inhibitory protein Bcl-x was studied in CD34+
hematopoietic precursor cells and in the promyelocytic leukemia cell line
HL-60. The enriched population of CD34+ cells (more than 95%) was cultured
in the presence of stem cell factor, interleukin-3 (IL-3), IL-6, and either
granulocyte colony-stimulating factor or macrophage colony-stimulating
factor to achieve granulocyte or monocyte/macrophage differentiation,
respectively. The expression of Bcl-x increased in the early stages of both
differentiation pathways. However, by day 21 of culture mature granulocytes
were Bcl-x-negative, whereas monocytes/macrophages either maintained or
increased the expression of Bcl-x. The pattern of Bcl-x expression in the
differentiated CD34+ cells was similar to that observed in HL-60 cells
differentiated along the granulocyte lineage (induced by incubation with
retinoic acid), or along the monocyte/macrophage lineage (induced by
incubation with phorbol diester). The bcl-x transcript predominant in HL-60
and CD34+ cells differentiated into monocytes/macrophages was bcl-xL.
Although little is yet known regarding the functional significance of Bcl-x
within the granulomonocytic compartment, marked changes in the pattern of
its expression, as observed during granulomonocytic differentiation of
HL-60 and CD34+ cells, are likely to alter the life span of mature
granulocytes and monocytes/macrophages.
Volume 89,
Issue 9,
pp. 3199-3204,
05/01/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Fontalba, O. Gutierrez, and J. L. Fernandez-Luna
NLRP2, an Inhibitor of the NF-{kappa}B Pathway, Is Transcriptionally Activated by NF-{kappa}B and Exhibits a Nonfunctional Allelic Variant
J. Immunol.,
December 15, 2007;
179(12):
8519 - 8524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Real, Y. Cao, R. Wang, Z. Nikolovska-Coleska, J. Sanz-Ortiz, S. Wang, and J. L. Fernandez-Luna
Breast Cancer Cells Can Evade Apoptosis-Mediated Selective Killing by a Novel Small Molecule Inhibitor of Bcl-2
Cancer Res.,
November 1, 2004;
64(21):
7947 - 7953.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Kimura, S. Suzu, Y. Nakamura, Y. Nakata, M. Yamada, N. Kuwada, T. Matsumura, T. Yamashita, T. Ikeda, K. Sato, et al.
Cloning and Characterization of a Novel RING-B-box-Coiled-coil Protein with Apoptotic Function
J. Biol. Chem.,
June 27, 2003;
278(27):
25046 - 25054.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. S. POLLOCK, J. TURCK, and D. H. LOVETT
The prodomain of interleukin 1{alpha} interacts with elements of the RNA processing apparatus and induces apoptosis in malignant cells
FASEB J,
February 1, 2003;
17(2):
203 - 213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Buitenhuis, B. Baltus, J.-W. J. Lammers, P. J. Coffer, and L. Koenderman
Signal transducer and activator of transcription 5a (STAT5a) is required for eosinophil differentiation of human cord blood-derived CD34+ cells
Blood,
January 1, 2003;
101(1):
134 - 142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Gutierrez, C. Pipaon, N. Inohara, A. Fontalba, Y. Ogura, F. Prosper, G. Nunez, and J. L. Fernandez-Luna
Induction of Nod2 in Myelomonocytic and Intestinal Epithelial Cells via Nuclear Factor-kappa B Activation
J. Biol. Chem.,
October 25, 2002;
277(44):
41701 - 41705.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kirito, T. Watanabe, K.-i. Sawada, H. Endo, K. Ozawa, and N. Komatsu
Thrombopoietin Regulates Bcl-xL Gene Expression through Stat5 and Phosphatidylinositol 3-Kinase Activation Pathways
J. Biol. Chem.,
March 1, 2002;
277(10):
8329 - 8337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R.-S. Charrad, Z. Gadhoum, J. Qi, A. Glachant, M. Allouche, C. Jasmin, C. Chomienne, and F. Smadja-Joffe
Effects of anti-CD44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines
Blood,
January 1, 2002;
99(1):
290 - 299.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Moulding, C. Akgul, M. Derouet, M. R. H. White, and S. W. Edwards
BCL-2 family expression in human neutrophils during delayed and accelerated apoptosis
J. Leukoc. Biol.,
November 1, 2001;
70(5):
783 - 792.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Lakics, A. E. Medvedev, S. Okada, and S. N. Vogel
Inhibition of LPS-induced Cytokines by Bcl-xL in a Murine Macrophage Cell Line
J. Immunol.,
September 1, 2000;
165(5):
2729 - 2737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Villunger, L. A. O'Reilly, N. Holler, J. Adams, and A. Strasser
Fas Ligand, Bcl-2, Granulocyte Colony-Stimulating Factor, and p38 Mitogen-activated Protein Kinase: Regulators of Distinct Cell Death and Survival Pathways in Granulocytes
J. Exp. Med.,
August 28, 2000;
192(5):
647 - 658.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Josefsen, J. H. Myklebust, J. Lømo, M. Sioud, H. K. Blomhoff, and E. B. Smeland
Differential Expression of Bcl-2 Homologs in Human CD34+ Hematopoietic Progenitor Cells Induced to Differentiate into Erythroid or Granulocytic Cells
Stem Cells,
July 1, 2000;
18(4):
261 - 272.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Sanz, A. Benito, N. Inohara, D. Ekhterae, G. Nunez, and J. L. Fernandez-Luna
Specific and rapid induction of the proapoptotic protein Hrk after growth factor withdrawal in hematopoietic progenitor cells
Blood,
May 1, 2000;
95(9):
2742 - 2747.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kanatani, T. Kasukabe, J. Okabe-Kado, Y. Yamamoto-Yamaguchi, N. Nagata, K. Motoyoshi, and Y. Honma
Role of CD14 Expression in the Differentiation-Apoptosis Switch in Human Monocytic Leukemia Cells Treated with 1{{alpha}},25-Dihydroxyvitamin D3 or Dexamethasone in the Presence of Transforming Growth Factor {beta}
Cell Growth Differ.,
October 1, 1999;
10(10):
705 - 712.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Oritani, Y. Tomiyama, P. W. Kincade, K. Aoyama, T. Yokota, I. Matsumura, Y. Kanakura, K. Nakajima, T. Hirano, and Y. Matsuzawa
Both Stat3-Activation and Stat3-Independent BCL2 Downregulation Are Important for Interleukin-6-Induced Apoptosis of 1A9-M Cells
Blood,
February 15, 1999;
93(4):
1346 - 1354.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Silva, C. Richard, A. Benito, C. Sanz, I. Olalla, and J. L. Fernandez-Luna
Expression of Bcl-x in Erythroid Precursors from Patients with Polycythemia Vera
N. Engl. J. Med.,
February 26, 1998;
338(9):
564 - 571.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. J. Ketley, P. D. Allen, S. M. Kelsey, and A. C. Newland
Modulation of Idarubicin-Induced Apoptosis in Human Acute Myeloid Leukemia Blasts by All-Trans Retinoic Acid, 1,25(OH)2 Vitamin D3, and Granulocyte-Macrophage Colony-Stimulating Factor
Blood,
December 1, 1997;
90(11):
4578 - 4587.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Sevilla, A. Zaldumbide, F. Carlotti, M. A. Dayem, P. Pognonec, and K. E. Boulukos
Bcl-xL Expression Correlates with Primary Macrophage Differentiation, Activation of Functional Competence, and Survival and Results from Synergistic Transcriptional Activation by Ets2 and PU.1
J. Biol. Chem.,
May 18, 2001;
276(21):
17800 - 17807.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
