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Focal adhesion kinase upregulated by granulocyte-macrophage colony-
stimulating factor but not by interleukin-3 in differentiating myeloid
cells
A Kume, H Nishiura, J Suda and T Suda
Department of Cell Differentiation, Kumamoto University School of Medicine,
Japan.
The involvement of focal adhesion kinase (FAK) in myeloid differentiation
was investigated in primary murine bone marrow (BM) cells. In unstimulated
BM, FAK mRNA was detected in myeloid and lymphoid cells, but not in
erythroid precursors. When the BM cells were incubated with
granulocyte-macrophage colony-stimulating factor (GM- CSF) or interleukin-3
(IL-3), FAK expression showed a remarkable difference depending on the
cytokine. Although FAK was upregulated in the cells stimulated by GM-CSF
(GM-treated cells), the kinase was barely detectable in the cells cultured
with IL-3 (IL-3-treated cells). Morphology and flow cytometry analysis
showed GM-CSF promoted the growth and differentiation of
monocyte/macrophage lineage stronger than IL-3. In addition, motility of
the cytokine-differentiated cells showed an overt distinction between the
cultures, which was closely correlated with FAK expression. After 7 days of
stimulation, GM-treated cells showed active migration and
chemoattractant-induced morphologic polarization. In contrast, IL-3-treated
cells showed minimal migration and polarization. These results suggest an
important role of GM-CSF in the terminal differentiation of
monocytes/macrophages, and possible involvement of FAK in functional
maturity of this lineage.
Volume 89,
Issue 9,
pp. 3434-3442,
05/01/1997
Copyright © 1997 by The American Society of Hematology
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