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Intracellular Pool of Vascular Endothelial Growth Factor in Human Neutrophils
Murielle Gaudry,
Olivier Brégerie,
Valérie Andrieu,
Jamel El Benna,
Marie-Anne Pocidalo, and
Jacques Hakim
From the Laboratoire d'Hématologie et d'Immunologie, INSERM U294, Faculté Xavier Bichat, Paris, France.
Vascular endothelial growth factor (VEGF ), an endothelial cell mitogen, is a potent angiogenic factor produced by several cell types. Whether human neutrophils are potential producers of VEGF has not yet been described. The present work shows that phorbol-12-myristate 13-acetate (PMA), fMet-Leu-Phe, and tumor necrosis factor- (TNF-) triggered a time-dependent secretion of VEGF by human neutrophils. Cells incubated with 50 ng/mL of PMA released significant amounts of VEGF after 15 minutes. Because the extracellular content of VEGF in human neutrophils supernatants remained constant over a period of 2 to 24 hours and because PMA is a potent inducer of human neutrophil degranulation, the PMA-induced secretion of VEGF may be due to a pre-existing intracellular pool of this molecule. This hypothesis was reinforced by the absence of cycloheximide effect on the PMA-induced secretion of VEGF. The existence of an intracellular pool of VEGF was confirmed by measuring the intracellular content of VEGF in resting neutrophils. A dosedependent inhibition of PMA-induced VEGF secretion was observed when the cells were incubated in the presence of pentoxifylline, a methylxanthine known to inhibit neutrophil degranulation. To confirm the implication of neutrophil degranulation in VEGF release, the effects of two inducers of physiologic degranulation, fMet-Leu-Phe and TNF-, were determined. Both agonists induced a release of VEGF in the absence of cytochalasin B, confirming the involvement of neutrophil degranulation and suggesting the intracellular localization of VEGF in the specific granule fraction. In addition, the kinetics of fMet-Leu-Phe- and TNF--induced secretion of lactoferrin were similar to those of VEGF release induced by these two both agonists. The subcellular fractionation of human neutrophils showed a granule-specific distribution of the intracellular pool of VEGF in resting neutrophils. The finding that human neutrophils contain an intracellular pool of VEGF, secreted in the extracellular space under PMA-, fMet-Leu-Phe-, and TNF--induced degranulation, suggests a role for human neutrophils as cellular effectors of physiologic as well as pathologic angiogenesis.
Blood, Vol. 90 No. 10 (November 15), 1997:
pp. 4153-4161
© 1997 by The American Society of Hematology.
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180(6):
4283 - 4291.
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May 1, 2007;
292(5):
L1147 - L1154.
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88(suppl_3):
140 - 147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-J. Na, S.-H. Yang, D.-W. Baek, D.-H. Lee, K.-H. Kim, Y.-M. Choi, S.-T. Oh, Y.-S. Hong, J.-Y. Kwak, and K.-S. Lee
Effects of peritoneal fluid from endometriosis patients on the release of vascular endothelial growth factor by neutrophils and monocytes
Hum. Reprod.,
July 1, 2006;
21(7):
1846 - 1855.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
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Y.-J. Lin, M.-D. Lai, H.-Y. Lei, and L.-Y. C. Wing
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Endocrinology,
March 1, 2006;
147(3):
1278 - 1286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Cassatella, V. Huber, F. Calzetti, D. Margotto, N. Tamassia, G. Peri, A. Mantovani, L. Rivoltini, and C. Tecchio
Interferon-activated neutrophils store a TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) intracellular pool that is readily mobilizable following exposure to proinflammatory mediators
J. Leukoc. Biol.,
January 1, 2006;
79(1):
123 - 132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Queen, R. E. Ryan, R. G. Holzer, C. R. Keller-Peck, and C. L. Jorcyk
Breast Cancer Cells Stimulate Neutrophils to Produce Oncostatin M: Potential Implications for Tumor Progression
Cancer Res.,
October 1, 2005;
65(19):
8896 - 8904.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. S. Barcelos, A. Talvani, A. S. Teixeira, L. Q. Vieira, G. D. Cassali, S. P. Andrade, and M. M. Teixeira
Impaired inflammatory angiogenesis, but not leukocyte influx, in mice lacking TNFR1
J. Leukoc. Biol.,
August 1, 2005;
78(2):
352 - 358.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J Teng, K. Lachapelle, and R. C. Chiu
Reappraisal of Recent Clinical Trials of Angiogenic Therapy in Myocardial Ischemia
Asian Cardiovasc Thorac Ann,
March 1, 2005;
13(1):
90 - 97.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Schruefer, N. Lutze, J. Schymeinsky, and B. Walzog
Human neutrophils promote angiogenesis by a paracrine feedforward mechanism involving endothelial interleukin-8
Am J Physiol Heart Circ Physiol,
March 1, 2005;
288(3):
H1186 - H1192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lemieux, R. Maliba, J. Favier, J.-F. Theoret, Y. Merhi, and M. G. Sirois
Angiopoietins can directly activate endothelial cells and neutrophils to promote proinflammatory responses
Blood,
February 15, 2005;
105(4):
1523 - 1530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mura, C. C. dos Santos, D. Stewart, and M. Liu
Vascular endothelial growth factor and related molecules in acute lung injury
J Appl Physiol,
November 1, 2004;
97(5):
1605 - 1617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sandhu, K. Teichert-Kuliszewska, S. Nag, G. Proteau, M. J. Robb, A. I.M. Campbell, M. A. Kuliszewski, M. J.B. Kutryk, and D. J. Stewart
Reciprocal regulation of angiopoietin-1 and angiopoietin-2 following myocardial infarction in the rat
Cardiovasc Res,
October 1, 2004;
64(1):
115 - 124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sakarya, S. Rifat, J. Zhou, D. D. Bannerman, N. M. Stamatos, A. S. Cross, and S. E. Goldblum
Mobilization of neutrophil sialidase activity desialylates the pulmonary vascular endothelial surface and increases resting neutrophil adhesion to and migration across the endothelium
Glycobiology,
June 1, 2004;
14(6):
481 - 494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B Heryanto, J E Girling, and P A W Rogers
Intravascular neutrophils partially mediate the endometrial endothelial cell proliferative response to oestrogen in ovariectomised mice
Reproduction,
May 1, 2004;
127(5):
613 - 620.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Scapini, M. Morini, C. Tecchio, S. Minghelli, E. Di Carlo, E. Tanghetti, A. Albini, C. Lowell, G. Berton, D. M. Noonan, et al.
CXCL1/Macrophage Inflammatory Protein-2-Induced Angiogenesis In Vivo Is Mediated by Neutrophil-Derived Vascular Endothelial Growth Factor-A
J. Immunol.,
April 15, 2004;
172(8):
5034 - 5040.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. De Bandt, M. H. Ben Mahdi, V. Ollivier, M. Grossin, M. Dupuis, M. Gaudry, P. Bohlen, K. E. Lipson, A. Rice, Y. Wu, et al.
Blockade of Vascular Endothelial Growth Factor Receptor I (VEGF-RI), but not VEGF-RII, Suppresses Joint Destruction in the K/BxN Model of Rheumatoid Arthritis
J. Immunol.,
November 1, 2003;
171(9):
4853 - 4859.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Burns, C. W. Smith, and D. C. Walker
Unique Structural Features That Influence Neutrophil Emigration Into the Lung
Physiol Rev,
April 1, 2003;
83(2):
309 - 336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Iba, H. Matsubara, Y. Nozawa, S. Fujiyama, K. Amano, Y. Mori, H. Kojima, and T. Iwasaka
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October 8, 2002;
106(15):
2019 - 2025.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. I. Resto-Ruiz, M. Schmiederer, D. Sweger, C. Newton, T. W. Klein, H. Friedman, and B. E. Anderson
Induction of a Potential Paracrine Angiogenic Loop between Human THP-1 Macrophages and Human Microvascular Endothelial Cells during Bartonella henselae Infection
Infect. Immun.,
August 1, 2002;
70(8):
4564 - 4570.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. S. Gambino, N. G. Wreford, J. F. Bertram, P. Dockery, F. Lederman, and P. A.W. Rogers
Angiogenesis occurs by vessel elongation in proliferative phase human endometrium
Hum. Reprod.,
May 1, 2002;
17(5):
1199 - 1206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Grenier, S. Chollet-Martin, B. Crestani, C. Delarche, J. El Benna, A. Boutten, V. Andrieu, G. Durand, M.-A. Gougerot-Pocidalo, M. Aubier, et al.
Presence of a mobilizable intracellular pool of hepatocyte growth factor in human polymorphonuclear neutrophils
Blood,
April 15, 2002;
99(8):
2997 - 3004.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Jaffre, M. Dehoux, C. Paugam, A. Grenier, S. Chollet-Martin, J.-B. Stern, J. Mantz, M. Aubier, and B. Crestani
Hepatocyte growth factor is produced by blood and alveolar neutrophils in acute respiratory failure
Am J Physiol Lung Cell Mol Physiol,
February 1, 2002;
282(2):
L310 - L315.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Maitre, S. Boussat, D. Jean, M. Gouge, L. Brochard, B. Housset, S. Adnot, and C. Delclaux
Vascular endothelial growth factor synthesis in the acute phase of experimental and clinical lung injury
Eur. Respir. J.,
July 1, 2001;
18(1):
100 - 106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Gargett, F. Lederman, B. Heryanto, L. S. Gambino, and P. A.W. Rogers
Focal vascular endothelial growth factor correlates with angiogenesis in human endometrium. Role of intravascular neutrophils
Hum. Reprod.,
June 1, 2001;
16(6):
1065 - 1075.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Elbim, H. Reglier, M. Fay, C. Delarche, V. Andrieu, J. El Benna, and M.-A. Gougerot-Pocidalo
Intracellular Pool of IL-10 Receptors in Specific Granules of Human Neutrophils: Differential Mobilization by Proinflammatory Mediators
J. Immunol.,
April 15, 2001;
166(8):
5201 - 5207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Seandel, K. Noack-Kunnmann, D. Zhu, R. T. Aimes, and J. P. Quigley
Growth factor-induced angiogenesis in vivo requires specific cleavage of fibrillar type I collagen
Blood,
April 15, 2001;
97(8):
2323 - 2332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. M. W. Verheul, A. S. Jorna, K. Hoekman, H. J. Broxterman, M. F. B. G. Gebbink, and H. M. Pinedo
Vascular endothelial growth factor-stimulated endothelial cells promote adhesion and activation of platelets
Blood,
December 15, 2000;
96(13):
4216 - 4221.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Lee, H. J. Yoon, Z. Zhu, H. Link, Z. Wang, J. M. Gwaltney Jr., M. Landry, and J. A. Elias
Respiratory Syncytial Virus Stimulation of Vascular Endothelial Cell Growth Factor/Vascular Permeability Factor
Am. J. Respir. Cell Mol. Biol.,
November 1, 2000;
23(5):
662 - 669.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Murakami, Y. Nakamura, K. Torikai, T. Tanaka, T. Koshiba, K. Koshimizu, S. Kuwahara, Y. Takahashi, K. Ogawa, M. Yano, et al.
Inhibitory Effect of Citrus Nobiletin on Phorbol Ester-induced Skin Inflammation, Oxidative Stress, and Tumor Promotion in Mice
Cancer Res.,
September 1, 2000;
60(18):
5059 - 5066.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. van der Flier, F. Coenjaerts, J. L. L. Kimpen, A. M. Hoepelman, and S. P. M. Geelen
Streptococcus pneumoniae Induces Secretion of Vascular Endothelial Growth Factor by Human Neutrophils
Infect. Immun.,
August 1, 2000;
68(8):
4792 - 4794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Koehne, C. Willam, E. Strauss, R. Schindler, K.-U. Eckardt, and C. Buhrer
Lack of hypoxic stimulation of VEGF secretion from neutrophils and platelets
Am J Physiol Heart Circ Physiol,
August 1, 2000;
279(2):
H817 - H824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Kaner, J. V. Ladetto, R. Singh, N. Fukuda, M. A. Matthay, and R. G. Crystal
Lung Overexpression of the Vascular Endothelial Growth Factor Gene Induces Pulmonary Edema
Am. J. Respir. Cell Mol. Biol.,
June 1, 2000;
22(6):
657 - 664.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Adams, P. J. Carder, S. Downey, M. A. Forbes, K. MacLennan, V. Allgar, S. Kaufman, S. Hallam, R. Bicknell, J. J. Walker, et al.
Vascular Endothelial Growth Factor (VEGF) in Breast Cancer: Comparison of Plasma, Serum, and Tissue VEGF and Microvessel Density and Effects of Tamoxifen
Cancer Res.,
June 1, 2000;
60(11):
2898 - 2905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Sakaguchi, N. Ikeda, M. Nakayama, Y. Kato, I. Yano, and K. Kaneda
Trehalose 6,6'-Dimycolate (Cord Factor) Enhances Neovascularization through Vascular Endothelial Growth Factor Production by Neutrophils and Macrophages
Infect. Immun.,
April 1, 2000;
68(4):
2043 - 2052.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Stefanec
Endothelial Apoptosis: Could It Have a Role in the Pathogenesis and Treatment of Disease?
Chest,
March 1, 2000;
117(3):
841 - 854.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. McCourt, J. H. Wang, S. Sookhai, and H. P. Redmond
Proinflammatory Mediators Stimulate Neutrophil-Directed Angiogenesis
Arch Surg,
December 1, 1999;
134(12):
1325 - 1331.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Vinante, M. Marchi, A. Rigo, P. Scapini, G. Pizzolo, and M. A. Cassatella
Granulocyte-Macrophage Colony-Stimulating Factor Induces Expression of Heparin-Binding Epidermal Growth Factor-Like Growth Factor/Diphtheria Toxin Receptor and Sensitivity to Diphtheria Toxin in Human Neutrophils
Blood,
November 1, 1999;
94(9):
3169 - 3177.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D R Thickett, L Armstrong, and A B Millar
Vascular endothelial growth factor (VEGF) in inflammatory and malignant pleural effusions
Thorax,
August 1, 1999;
54(8):
707 - 710.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Cao, M. K. Mathews, D S. McLeod, C. Merges, L. M Hjelmeland, and G. A Lutty
Angiogenic factors in human proliferative sickle cell retinopathy
Br J Ophthalmol,
July 1, 1999;
83(7):
838 - 846.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Berkova, C. Gilbert, S. Goupil, J. Yan, V. Korobko, and P. H. Naccache
TNF-Induced Haptoglobin Release from Human Neutrophils: Pivotal Role of the TNF p55 Receptor
J. Immunol.,
May 15, 1999;
162(10):
6226 - 6232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Grenier, M. Dehoux, A. Boutten, M. Arce-Vicioso, G. Durand, M.-A. Gougerot-Pocidalo, and S. Chollet-Martin
Oncostatin M Production and Regulation by Human Polymorphonuclear Neutrophils
Blood,
February 15, 1999;
93(4):
1413 - 1421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Giraudo, L. Primo, E. Audero, H.-P. Gerber, P. Koolwijk, S. Soker, M. Klagsbrun, N. Ferrara, and F. Bussolino
Tumor Necrosis Factor-alpha Regulates Expression of Vascular Endothelial Growth Factor Receptor-2 and of Its Co-receptor Neuropilin-1 in Human Vascular Endothelial Cells
J. Biol. Chem.,
August 21, 1998;
273(34):
22128 - 22135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Kevil, D. K. Payne, E. Mire, and J. S. Alexander
Vascular Permeability Factor/Vascular Endothelial Cell Growth Factor-mediated Permeability Occurs through Disorganization of Endothelial Junctional Proteins
J. Biol. Chem.,
June 12, 1998;
273(24):
15099 - 15103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Ollivier, S. Bentolila, J. Chabbat, J. Hakim, and D. de Prost
Tissue Factor-Dependent Vascular Endothelial Growth Factor Production by Human Fibroblasts in Response to Activated Factor VII
Blood,
April 15, 1998;
91(8):
2698 - 2703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
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