|
|
 Previous Article | Table of Contents | Next Article 
The Role of Interleukin-10 (IL-10) in IL-15-Mediated T-Cell Responses
Dieter Körholz,
Ursula Banning,
Halvard Bönig,
Markus Grewe,
Marion Schneider,
Christine Mauz-Körholz,
Anne Klein-Vehne,
Jean Krutmann, and
Stephan Burdach
From the Departments of Pediatric Hematology and Oncology and Dermatology, Laboratory of Immunology, the Department of Transfusion Medicine and Hemostaseology, Heinrich-Heine University Medical Center, Biomedical Research Center Heinrich-Heine University, Düsseldorf, Germany.
Interleukin-15 (IL-15) is a potent T-cell stimulating factor, which has recently been used for pre-clinical in vivo immunotherapy. Here, the IL-15 effect on CD3-stimulated peripheral human T cells was investigated. IL-15 induced a significant T-cell proliferation and upregulated CD25 expression. IL-15 significantly enhanced T-cell production of interferon- (IFN-), tumor necrosis factor- (TNF-), and IL-10. Between 10- and 100-fold greater concentrations of IL-15 were necessary to reach a biological effect equivalent to that of IL-2. Blockade of IL-2 binding to the high-affinity IL-2 receptor did not affect the IL-15 effects, suggesting that IL-15 did not act by inducing endogenous IL-2. Exogenously administered IL-10 significantly reduced the IL-15 and IL-2-mediated IFN- and TNF- production, whereas T-cell proliferation and CD25 expression were not affected. The inhibitory effects of exogenously administered IL-10 on T-cell cytokine production appeared indirect, and are likely secondary to decreased IL-12 production by accessory cells. Inhibition of endogenous IL-10 binding to the IL-10 receptor significantly increased IFN- and TNF- release from T cells. These data suggest that endogenous IL-10 can regulate activated T-cell production of IFN- and TNF- via a paracrine negative feedback loop. The observations of this study could be of relevance for the therapeutic use of IL-15 in vivo.
Blood, Vol. 90 No. 11 (December 1), 1997:
pp. 4513-4521
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. I. Kokaji, D. L. Hockley, and K. P. Kane
IL-15 Transpresentation Augments CD8+ T Cell Activation and Is Required for Optimal Recall Responses by Central Memory CD8+ T Cells
J. Immunol.,
April 1, 2008;
180(7):
4391 - 4401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pauschinger, S. Rutschow, K. Chandrasekharan, D. Westermann, A. Weitz, L. P. Schwimmbeck, H. Zeichhardt, W. Poller, M. Noutsias, J. Li, et al.
Carvedilol improves left ventricular function in murine coxsackievirus-induced acute myocarditis Association with reduced myocardial interleukin-1{beta} and MMP-8 expression and a modulated immune response
Eur J Heart Fail,
June 1, 2005;
7(4):
444 - 452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Gorgun, K. B. Miller, and F. M. Foss
Immunologic mechanisms of extracorporeal photochemotherapy in chronic graft-versus-host disease
Blood,
July 18, 2002;
100(3):
941 - 947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Dunne, S. Lynch, C. O'Farrelly, S. Todryk, J. E. Hegarty, C. Feighery, and D. G. Doherty
Selective Expansion and Partial Activation of Human NK Cells and NK Receptor-Positive T Cells by IL-2 and IL-15
J. Immunol.,
September 15, 2001;
167(6):
3129 - 3138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. I. Chapoval, J. A. Fuller, S. G. Kremlev, S. J. Kamdar, and R. Evans
Combination Chemotherapy and IL-15 Administration Induce Permanent Tumor Regression in a Mouse Lung Tumor Model: NK and T Cell-Mediated Effects Antagonized by B Cells
J. Immunol.,
December 15, 1998;
161(12):
6977 - 6984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-N. Avice, C. E. Demeure, G. Delespesse, M. Rubio, M. Armant, and M. Sarfati
IL-15 Promotes IL-12 Production by Human Monocytes Via T Cell-Dependent Contact and May Contribute to IL-12-Mediated IFN-{gamma} Secretion by CD4+ T Cells in the Absence of TCR Ligation
J. Immunol.,
October 1, 1998;
161(7):
3408 - 3415.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
