|
|
 Previous Article | Table of Contents | Next Article 
Cytokine Expression and Tumorigenicity of Large Granular Lymphocytic Leukemia Cells From Mice Transgenic for the tax Gene of Human T-Cell Leukemia Virus Type I
William J. Grossman and
Lee Ratner
From the Departments of Medicine, Molecular Microbiology, and Pathology, Washington University School of Medicine, St Louis, MO.
The human T-cell leukemia virus type I (HTLV-I) regulatory protein, Tax, has been speculated to play a major role in HTLV-I leukemogenesis. Indeed, several studies have suggested that upregulation of various cellular oncogenes and cytokines by Tax may explain the pathogenesis observed in HTLV-I-infected individuals, as well as several Tax-transgenic animal models. We report here the analysis of cytokine expression in a Tax-transgenic animal model with large granular lymphocytic (LGL) leukemia. Two different transgenic mice showed identical expression of interleukin-1 (IL-1), IL-1 , interferon  (IFN), and granulocyte-macrophage colony-stimulating factor (GM-CSF ) in peripheral tail tumors. Interestingly, LGL cell lines derived from these same tumors expressed high levels of both IFN and GM-CSF, which correlated with the level of Tax expression. These same LGL cell lines also expressed high levels of lymphocyte function-associated antigen-1 (LFA-1) and intracellular adhesion molecule-1 (ICAM-1). Engraftment of these LGL cell lines into severe combined immunodeficient (SCID) mice led to the development of leukemia and lymphomas. Examination of these SCID mice showed that their pathology was nearly identical to that observed in the original Tax-transgenic mouse model. Both the Tax-transgenic and engrafted SCID mouse models allow for the analysis of cellular events that are required for tumor development associated with HTLV infection and suggest that Tax expression may be responsible for the upregulation of certain cytokines and adhesion molecules that affect the infiltrating capabilities of HTLV-I-infected cells.
Blood, Vol. 90 No. 2 (July 15), 1997:
pp. 783-794
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Rauch, S. Gross, J. Harding, S. Niewiesk, M. Lairmore, D. Piwnica-Worms, and L. Ratner
Imaging spontaneous tumorigenesis: inflammation precedes development of peripheral NK tumors
Blood,
February 12, 2009;
113(7):
1493 - 1500.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Bernal-Mizrachi, C. M. Lovly, and L. Ratner
The role of NF-{kappa}B-1 and NF-{kappa}B-2-mediated resistance to apoptosis in lymphomas
PNAS,
June 13, 2006;
103(24):
9220 - 9225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mitra-Kaushik, J. Harding, J. Hess, R. Schreiber, and L. Ratner
Enhanced tumorigenesis in HTLV-1 Tax-transgenic mice deficient in interferon-gamma
Blood,
November 15, 2004;
104(10):
3305 - 3311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lima, J. Almeida, A. G. Montero, M. d. A. Teixeira, M. L. Queiros, A. H. Santos, A. Balanzategui, A. Estevinho, M. d. C. Alguero, P. Barcena, et al.
Clinicobiological, Immunophenotypic, and Molecular Characteristics of Monoclonal CD56-/+dim Chronic Natural Killer Cell Large Granular Lymphocytosis
Am. J. Pathol.,
October 1, 2004;
165(4):
1117 - 1127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mitra-Kaushik, J. C. Harding, J. L. Hess, and L. Ratner
Effects of the proteasome inhibitor PS-341 on tumor growth in HTLV-1 Tax transgenic mice and Tax tumor transplants
Blood,
August 1, 2004;
104(3):
802 - 809.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Portis, J. C. Harding, and L. Ratner
The contribution of NF-{kappa}B activity to spontaneous proliferation and resistance to apoptosis in human T-cell leukemia virus type 1 Tax-induced tumors
Blood,
August 15, 2001;
98(4):
1200 - 1208.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Portis, W. J. Grossman, J. C. Harding, J. L. Hess, and L. Ratner
Analysis of p53 Inactivation in a Human T-Cell Leukemia Virus Type 1 Tax Transgenic Mouse Model
J. Virol.,
March 1, 2001;
75(5):
2185 - 2193.
[Abstract]
[Full Text]
|
|
|
|
|
