|
|
 Previous Article | Table of Contents | Next Article 
Human Monocytes and Neutrophils Store Transforming Growth Factor- in a Subpopulation of Cytoplasmic Granules
Jero Calafat,
Hans Janssen,
Mona Stå hle-Bäckdahl,
Astrid E.M. Zuurbier,
Edward F. Knol, and
Arne Egesten
From the Division of Cell Biology, Netherlands Cancer Institute, Amsterdam; Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands; Department of Dermatology, Karolinska Hospital, Stockholm; and Department of Medicine, University Hospital MAS, Malmö, Sweden.
Transforming growth factor- (TGF-) exerts several effects on target cells, such as neovascularization promotion and mitogenic signalling. Using immunoelectron microscopy, we show that monocytes and neutrophils, store TGF- in cytoplasmic granules. In monocytes, TGF- did not colocalize with components of peroxidase-positive granules or with albumin of secretory vesicles. Furthermore, no colocalization of TGF- with components of azurophilic or specific granules or secretory vesicles was observed in neutrophils. Activated monocytes and tissue-macrophages contained much less TGF--positive granules, suggesting TGF- release. Western blot analysis showed a protein of 10 kD in lysates of monocytes. TGF- mRNA was detected in monocytoid cells from the bone marrow by in situ hybridization. This study shows for the first time that monocytes and neutrophils contain TGF- in all stages of maturation and that TGF- in monocytes is stored in a large population of peroxidase-negative granules suggesting a function for these granules. Monocytes and neutrophils are important effector cells in inflammatory reactions. The present finding that these cells contain TGF- might explain complications such as fibrosis and neoplastic transformation, caused by chronic inflammation.
Blood, Vol. 90 No. 3 (August 1), 1997:
pp. 1255-1266
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
N. Rocha, C. Kuijl, R. van der Kant, L. Janssen, D. Houben, H. Janssen, W. Zwart, and J. Neefjes
Cholesterol sensor ORP1L contacts the ER protein VAP to control Rab7-RILP-p150Glued and late endosome positioning
J. Cell Biol.,
June 29, 2009;
185(7):
1209 - 1225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. D. Bannerman
Pathogen-dependent induction of cytokines and other soluble inflammatory mediators during intramammary infection of dairy cows
J Anim Sci,
April 1, 2009;
87(13_suppl):
10 - 25.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bunbury, I. Potolicchio, R. Maitra, and L. Santambrogio
Functional analysis of monocyte MHC class II compartments
FASEB J,
January 1, 2009;
23(1):
164 - 171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Kallquist, M. Hansson, A.-M. Persson, H. Janssen, J. Calafat, H. Tapper, and I. Olsson
The tetraspanin CD63 is involved in granule targeting of neutrophil elastase
Blood,
October 15, 2008;
112(8):
3444 - 3454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P-R. Burgel and J. A. Nadel
Epidermal growth factor receptor-mediated innate immune responses and their roles in airway diseases
Eur. Respir. J.,
October 1, 2008;
32(4):
1068 - 1081.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Herrero-Turrion, J. Calafat, H. Janssen, M. Fukuda, and F. Mollinedo
Rab27a Regulates Exocytosis of Tertiary and Specific Granules in Human Neutrophils
J. Immunol.,
September 15, 2008;
181(6):
3793 - 3803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ketema, K. Wilhelmsen, I. Kuikman, H. Janssen, D. Hodzic, and A. Sonnenberg
Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin
J. Cell Sci.,
October 1, 2007;
120(19):
3384 - 3394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gomez-Gaviro, M. Dominguez-Luis, J. Canchado, J. Calafat, H. Janssen, E. Lara-Pezzi, A. Fourie, A. Tugores, A. Valenzuela-Fernandez, F. Mollinedo, et al.
Expression and Regulation of the Metalloproteinase ADAM-8 during Human Neutrophil Pathophysiological Activation and Its Catalytic Activity on L-Selectin Shedding
J. Immunol.,
June 15, 2007;
178(12):
8053 - 8063.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Keller, T. A. Groothuis, E. A. M. Veraar, M. Marsman, L. M. de Buy Wenniger, H. Janssen, J. Neefjes, and J. Borst
Costimulatory ligand CD70 is delivered to the immunological synapse by shared intracellular trafficking with MHC class II molecules
PNAS,
April 3, 2007;
104(14):
5989 - 5994.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Masztalerz, I. S. Zeelenberg, Y. M. Wijnands, R. de Bruijn, A. M. Drager, H. Janssen, and E. Roos
Synaptotagmin 3 deficiency in T cells impairs recycling of the chemokine receptor CXCR4 and thereby inhibits CXCL12 chemokine-induced migration
J. Cell Sci.,
January 15, 2007;
120(2):
219 - 228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Vidarsson, A. M. Stemerding, N. M. Stapleton, S. E. Spliethoff, H. Janssen, F. E. Rebers, M. de Haas, and J. G. van de Winkel
FcRn: an IgG receptor on phagocytes with a novel role in phagocytosis
Blood,
November 15, 2006;
108(10):
3573 - 3579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mollinedo, J. Calafat, H. Janssen, B. Martin-Martin, J. Canchado, S. M. Nabokina, and C. Gajate
Combinatorial SNARE Complexes Modulate the Secretion of Cytoplasmic Granules in Human Neutrophils.
J. Immunol.,
September 1, 2006;
177(5):
2831 - 2841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Theilgaard-Monch, L. C. Jacobsen, M. J. Nielsen, T. Rasmussen, L. Udby, M. Gharib, P. D. Arkwright, A. F. Gombart, J. Calafat, S. K. Moestrup, et al.
Haptoglobin is synthesized during granulocyte differentiation, stored in specific granules, and released by neutrophils in response to activation
Blood,
July 1, 2006;
108(1):
353 - 361.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Potolicchio, S. Chitta, X. Xu, D. Fonseca, G. Crisi, V. Horejsi, J. L. Strominger, L. J. Stern, G. Raposo, and L. Santambrogio
Conformational Variation of Surface Class II MHC Proteins during Myeloid Dendritic Cell Differentiation Accompanies Structural Changes in Lysosomal MIIC
J. Immunol.,
October 15, 2005;
175(8):
4935 - 4947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Martin, R. Wallich, J. Pardo, A. Mullbacher, M. Munder, M. Modolell, and M. M. Simon
Quiescent and activated mouse granulocytes do not express granzyme A and B or perforin: similarities or differences with human polymorphonuclear leukocytes?
Blood,
October 15, 2005;
106(8):
2871 - 2878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Faurschou, S. Kamp, J. B. Cowland, L. Udby, A. H. Johnsen, J. Calafat, H. Winther, and N. Borregaard
Prodefensins are matrix proteins of specific granules in human neutrophils
J. Leukoc. Biol.,
September 1, 2005;
78(3):
785 - 793.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. C. W. Poland, J.-J. G. Vallejo, H. W. M. Niessen, R. Nijmeyer, J. Calafat, C. E. Hack, B. Van het Hof, and W. Van Dijk
Activated human PMN synthesize and release a strongly fucosylated glycoform of {alpha}1-acid glycoprotein, which is transiently deposited in human myocardial infarction
J. Leukoc. Biol.,
August 1, 2005;
78(2):
453 - 461.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Theilgaard-Monch, L. C. Jacobsen, T. Rasmussen, C. U. Niemann, L. Udby, R. Borup, M. Gharib, P. D. Arkwright, A. F. Gombart, J. Calafat, et al.
Highly glycosylated {alpha}1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils
J. Leukoc. Biol.,
August 1, 2005;
78(2):
462 - 470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Chockalingam, M. J. Paape, and D. D. Bannerman
Increased Milk Levels of Transforming Growth Factor-{alpha}, {beta}1, and {beta}2 During Escherichia coli-Induced Mastitis
J Dairy Sci,
June 1, 2005;
88(6):
1986 - 1993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. E. Sorensen, D. R. Thapa, A. Rosenthal, L. Liu, A. A. Roberts, and T. Ganz
Differential Regulation of {beta}-Defensin Expression in Human Skin by Microbial Stimuli
J. Immunol.,
April 15, 2005;
174(8):
4870 - 4879.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Munder, F. Mollinedo, J. Calafat, J. Canchado, C. Gil-Lamaignere, J. M. Fuentes, C. Luckner, G. Doschko, G. Soler, K. Eichmann, et al.
Arginase I is constitutively expressed in human granulocytes and participates in fungicidal activity
Blood,
March 15, 2005;
105(6):
2549 - 2556.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Gao, M. Hansson, J. Calafat, H. Tapper, and I. Olsson
Sorting soluble tumor necrosis factor (TNF) receptor for storage and regulated secretion in hematopoietic cells
J. Leukoc. Biol.,
October 1, 2004;
76(4):
876 - 885.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-H. Kim, K.-H. Jung, J.-H. Han, J.-J. Shim, K.-H. In, K.-H. Kang, and S.-H. Yoo
Relation of Epidermal Growth Factor Receptor Expression to Mucus Hypersecretion in Diffuse Panbronchiolitis
Chest,
September 1, 2004;
126(3):
888 - 895.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. U. Niemann, J. B. Cowland, P. Klausen, J. Askaa, J. Calafat, and N. Borregaard
Localization of serglycin in human neutrophil granulocytes and their precursors
J. Leukoc. Biol.,
August 1, 2004;
76(2):
406 - 415.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Gao, H. Rosen, E. Johnsson, J. Calafat, H. Tapper, and I. Olsson
Sorting of soluble TNF-receptor for granule storage in hematopoietic cells as a principle for targeting of selected proteins to inflamed sites
Blood,
July 15, 2003;
102(2):
682 - 688.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. E. Sorensen, J. B. Cowland, K. Theilgaard-Monch, L. Liu, T. Ganz, and N. Borregaard
Wound Healing and Expression of Antimicrobial Peptides/Polypeptides in Human Keratinocytes, a Consequence of Common Growth Factors
J. Immunol.,
June 1, 2003;
170(11):
5583 - 5589.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Persson-Dajotoy, P. Andersson, A. Bjartell, J. Calafat, and A. Egesten
Expression and Production of the CXC Chemokine Growth-Related Oncogene-{alpha} by Human Eosinophils
J. Immunol.,
May 15, 2003;
170(10):
5309 - 5316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mollinedo, B. Martin-Martin, J. Calafat, S. M. Nabokina, and P. A. Lazo
Role of Vesicle-Associated Membrane Protein-2, Through Q-Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor/R-Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor Interaction, in the Exocytosis of Specific and Tertiary Granules of Human Neutrophils
J. Immunol.,
January 15, 2003;
170(2):
1034 - 1042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Udby, J. Calafat, O. E. Sorensen, N. Borregaard, and L. Kjeldsen
Identification of human cysteine-rich secretory protein 3 (CRISP-3) as a matrix protein in a subset of peroxidase-negative granules of neutrophils and in the granules of eosinophils
J. Leukoc. Biol.,
September 1, 2002;
72(3):
462 - 469.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Bulow, N. Bengtsson, J. Calafat, U. Gullberg, and I. Olsson
Sorting of neutrophil-specific granule protein human cathelicidin, hCAP-18, when constitutively expressed in myeloid cells
J. Leukoc. Biol.,
July 1, 2002;
72(1):
147 - 153.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Canonne-Hergaux, J. Calafat, E. Richer, M. Cellier, S. Grinstein, N. Borregaard, and P. Gros
Expression and subcellular localization of NRAMP1 in human neutrophil granules
Blood,
June 17, 2002;
100(1):
268 - 275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Bulow, W. M. Nauseef, M. Goedken, S. McCormick, J. Calafat, U. Gullberg, and I. Olsson
Sorting for storage in myeloid cells of nonmyeloid proteins and chimeras with the propeptide of myeloperoxidase precursor
J. Leukoc. Biol.,
February 1, 2002;
71(2):
279 - 288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. G. Neely, S. M. Robbins, E. K. Amankwah, S. Epelman, H. Wong, J. C. L. Spurrell, K. K. Jandu, W. Zhu, D. K. Fogg, C. B. Brown, et al.
Lipopolysaccharide-Stimulated or Granulocyte-Macrophage Colony-Stimulating Factor-Stimulated Monocytes Rapidly Express Biologically Active IL-15 on Their Cell Surface Independent of New Protein Synthesis
J. Immunol.,
November 1, 2001;
167(9):
5011 - 5017.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. de Melker, G. van der Horst, J. Calafat, H. Jansen, and J. Borst
c-Cbl ubiquitinates the EGF receptor at the plasma membrane and remains receptor associated throughout the endocytic route
J. Cell Sci.,
January 6, 2001;
114(11):
2167 - 2178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M.Th. Sterk, C. A.W. Geuijen, L. C.J.M. Oomen, J. Calafat, H. Janssen, and A. Sonnenberg
The Tetraspan Molecule Cd151, a Novel Constituent of Hemidesmosomes, Associates with the Integrin {alpha}6{beta}4 and May Regulate the Spatial Organization of Hemidesmosomes
J. Cell Biol.,
May 15, 2000;
149(4):
969 - 982.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Takeyama, K. Dabbagh, J. Jeong Shim, T. Dao-Pick, I. F. Ueki, and J. A. Nadel
Oxidative Stress Causes Mucin Synthesis Via Transactivation of Epidermal Growth Factor Receptor: Role of Neutrophils
J. Immunol.,
February 1, 2000;
164(3):
1546 - 1552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Cox, D. J. Lee, B. M. Dale, J. Calafat, and S. Greenberg
A Rab11-containing rapidly recycling compartment in macrophages that promotes phagocytosis
PNAS,
January 18, 2000;
97(2):
680 - 685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-M. Lee, K. Takeyama, K. Dabbagh, J. A. Lausier, I. F. Ueki, and J. A. Nadel
Agarose plug instillation causes goblet cell metaplasia by activating EGF receptors in rat airways
Am J Physiol Lung Cell Mol Physiol,
January 1, 2000;
278(1):
L185 - L192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Zhang, J. Calafat, H. Janssen, and S. Greenberg
ARF6 Is Required for Growth Factor- and Rac-Mediated Membrane Ruffling in Macrophages at a Stage Distal to Rac Membrane Targeting
Mol. Cell. Biol.,
December 1, 1999;
19(12):
8158 - 8168.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Vinante, M. Marchi, A. Rigo, P. Scapini, G. Pizzolo, and M. A. Cassatella
Granulocyte-Macrophage Colony-Stimulating Factor Induces Expression of Heparin-Binding Epidermal Growth Factor-Like Growth Factor/Diphtheria Toxin Receptor and Sensitivity to Diphtheria Toxin in Human Neutrophils
Blood,
November 1, 1999;
94(9):
3169 - 3177.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Takeyama, K. Dabbagh, H.-M. Lee, C. Agusti, J. A. Lausier, I. F. Ueki, K. M. Grattan, and J. A. Nadel
Epidermal growth factor system regulates mucin production in airways
PNAS,
March 16, 1999;
96(6):
3081 - 3086.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Grenier, M. Dehoux, A. Boutten, M. Arce-Vicioso, G. Durand, M.-A. Gougerot-Pocidalo, and S. Chollet-Martin
Oncostatin M Production and Regulation by Human Polymorphonuclear Neutrophils
Blood,
February 15, 1999;
93(4):
1413 - 1421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
