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RAPID COMMUNICATION
Upregulation of the Elongation Factor-1 Gene by p53 in Association With Death of an Erythroleukemic Cell Line
Mitsuo V. Kato,
Hiromi Sato,
Mariko Nagayoshi, and
Yoji Ikawa
From the Molecular Oncology Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Koyadai, Tsukuba, Ibaraki, Japan; the Department of Retroviral Regulation, Medical Research Division, Tokyo Medical and Dental University, Tokyo, Japan.
Genes upregulated by p53 were screened using an erythroleukemic cell line (1-2-3) that expresses only the temperature-sensitive p53 by the mRNA differential display method. One of the upregulated genes was identified as the elongation factor-1 (EF-1) gene, an essential component of the eukaryotic translation apparatus. Three p53-responsive elements were found in the mouse EF-1 gene and in the corresponding human, rat, and frog genes. These elements conferred the capacity for induction by p53. EF-1 is also a microtubule-severing protein. Upon the temperature-shift, the cells developed the morphology and the localization of -tubulin similar to those of the cells treated with vincristine, a drug that affects microtubules. The microtubule-severing associated with upregulation of EF-1 by p53 may be a cause of the cell death.
Blood, Vol. 90 No. 4 (August 15), 1997:
pp. 1373-1378
© 1997 by The American Society of Hematology.
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