|
|
 Previous Article | Table of Contents | Next Article 
Fluid Shear Stress Modulates von Willebrand Factor Release From Human Vascular Endothelium
Miriam Galbusera,
Carla Zoja,
Roberta Donadelli,
Simona Paris,
Marina Morigi,
Ariela Benigni,
Marina Figliuzzi,
Giuseppe Remuzzi, and
Andrea Remuzzi
From the Mario Negri Institute for Pharmacological Research and Division of Nephrology and Dialysis, Azienda Ospedaliera, Ospedali Riuniti di Bergamo, Bergamo, Italy.
Fluid shear stress generated by blood flow on arterial wall may play a role in the process of atherosclerosis, not only affecting the mass transport phenomena that take place in blood, but also by modulation of synthesis and secretion of humoral factors released by vascular endothelium that mediate platelet-vessel wall interactions. The present study was designed to investigate whether shear stress, induced by laminar flow, modulates von Willebrand factor (vWF ) release from cultured human umbilical vein endothelial cells (HUVEC) and whether this physical stimulation can affect vWF synthesis. Monolayers of HUVEC were exposed to laminar flow of varying magnitude (from 2 to 12 dynes/cm2) using a cone-and-plate device. The release of vWF in cell supernatant and in extracellular matrix by cells exposed to flow or maintained in static conditions was evaluated by enzyme-linked immunosorbent assay. HUVEC exposed to laminar flow released higher amounts of vWF into the cell supernatant within few hours of exposure and vWF secretion was dependent on shear stress magnitude. vWF released in extracellular matrix was also higher in cell monolayers exposed to shear than in static controls. vWF mRNA expression in HUVEC was not affected by exposure of cells to laminar flow, indicating that shear-induced vWF release reflected enhanced secretion without de novo protein synthesis. Immunofluorescence studies showed that the release of vWF is due to exocytosis from Weibel-Palade bodies, the storage organelles of vWF. These data indicate a novel mechanism by which local hemodynamic shear forces modulate endothelial cell function and may play a role in development of arterial thrombotic events.
Blood, Vol. 90 No. 4 (August 15), 1997:
pp. 1558-1564
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. K. Chauhan, J. Kisucka, A. Brill, M. T. Walsh, F. Scheiflinger, and D. D. Wagner
ADAMTS13: a new link between thrombosis and inflammation
J. Exp. Med.,
September 1, 2008;
205(9):
2065 - 2074.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Masoud, G. Sarig, B. Brenner, and G. Jacob
Orthostatic Hypercoagulability: A Novel Physiological Mechanism to Activate the Coagulation System
Hypertension,
June 1, 2008;
51(6):
1545 - 1551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Dangaria and P. J. Butler
Macrorheology and adaptive microrheology of endothelial cells subjected to fluid shear stress
Am J Physiol Cell Physiol,
November 1, 2007;
293(5):
C1568 - C1575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Brakemeier, A. Kersten, I. Eichler, I. Grgic, A. Zakrzewicz, H. Hopp, R. Kohler, and J. Hoyer
Shear stress-induced up-regulation of the intermediate-conductance Ca2+-activated K+ channel in human endothelium
Cardiovasc Res,
December 1, 2003;
60(3):
488 - 496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamamoto, T. Sokabe, N. Ohura, H. Nakatsuka, A. Kamiya, and J. Ando
Endogenously released ATP mediates shear stress-induced Ca2+ influx into pulmonary artery endothelial cells
Am J Physiol Heart Circ Physiol,
July 11, 2003;
285(2):
H793 - H803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Methia, P. Andre, C. V. Denis, M. Economopoulos, and D. D. Wagner
Localized reduction of atherosclerosis in von Willebrand factor-deficient mice
Blood,
September 1, 2001;
98(5):
1424 - 1428.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. W. Kamphuisen, J. C. J. Eikenboom, and R. M. Bertina
Elevated Factor VIII Levels and the Risk of Thrombosis
Arterioscler Thromb Vasc Biol,
May 1, 2001;
21(5):
731 - 738.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Altman, J. Rouvier, and A. Scazziota
State-of-the-Art Review : Secondary Prevention of Myocardial Infarction: Beneficial Effect of Combining Oral Anticoagulant Plus Aspirin: Therapy Based on Evidence
Clinical and Applied Thrombosis/Hemostasis,
July 1, 2000;
6(3):
126 - 134.
[PDF]
|
|
|
|
|
|
|
G. R. Y. De Meyer, M. F. Hoylaerts, M. M. Kockx, H. Yamamoto, A. G. Herman, and H. Bult
Intimal Deposition of Functional von Willebrand Factor in Atherogenesis
Arterioscler Thromb Vasc Biol,
October 1, 1999;
19(10):
2524 - 2534.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. D. Stamer, B. C. Roberts, and D. L. Epstein
Hydraulic Pressure Stimulates Adenosine 3',5'-Cyclic Monophosphate Accumulation in Endothelial Cells from Schlemm’s Canal
Invest. Ophthalmol. Vis. Sci.,
August 1, 1999;
40(9):
1983 - 1988.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mazzucato, P. Spessotto, A. Masotti, L. De Appollonia, M. R. Cozzi, A. Yoshioka, R. Perris, A. Colombatti, and L. De Marco
Identification of Domains Responsible for von Willebrand Factor Type VI Collagen Interaction Mediating Platelet Adhesion under High Flow
J. Biol. Chem.,
January 29, 1999;
274(5):
3033 - 3041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
